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The map label for this gene is carA [H]

Identifier: 162149108

GI number: 162149108

Start: 3431239

End: 3432642

Strand: Direct

Name: carA [H]

Synonym: GDI_3338

Alternate gene names: 162149108

Gene position: 3431239-3432642 (Clockwise)

Preceding gene: 162149099

Following gene: 162149109

Centisome position: 87.0

GC content: 72.08

Gene sequence:

>1404_bases
ATGCCGATATCGATCGAGACCATCATCGAGCGTCTGGGCGGCGCGGATCATGCCGCCCGGCTGACCGGCGTCGGGACCGA
GGCCATCCGCAAATGGCGGCAGGCCCGCGCGATCCCGCCCAAGCATTGGACGGTCATCCTGCGCCACACCGGCCTCAGCC
TTTCCGACCTGCAACCCGACAGCGCGTCCGACCGAGCGGAGACCCAGATGCCGGAGACCCCGTTACCCCCCGCCACCCAG
CCCCCCGAAGGCGCCACCGCCGCGCTGGTCCTGGCCGACGGCACGGTCGCGTGGGGGCGCGGCTTCGGCGCGCATACGCC
GGCCGCCGGCAGCGCCATCGGCGAACTGTGCTTTTCCACCGGCATGACCGGCTATCAGGAAACCCTGACCGATCCCTCCT
TCGCCGGGCAGATCATCACCTTCACCTTTCCCCATATCGGCAATGTCGGCACCAACGCGGATGACGACGAAGCCCCCCGC
GTGGCCGCGCGCGGGCTGGCGGTCAAGCAGGACCTGACCGAGCCCGCCAACTGGCGCGCGACGCAGGGGCTGGACGCCTG
GCTGGCCGGCCAGGGCGTGCCGGGCATCTGCGGCGTCGATACCCGCGCCATCACGCTGCGGGTGCGCGACGGCGGCCCGC
AGACCGCCATCCTGGCCTACCCCGCCGACGGCGTGTTCGACCTGGACGCCCTGCGTGCCCAGGCCGCCGCATGGCCGGGG
CTGGAAGGCATGGACCTGGCCCGCGACGTGACCTGCGCCGCCCCCTATTCCTGGGACAAGGGCGTCTGGACCTGGCCCGC
GGGCACCTGCCCGCTGCCCGAGCGCCGCCGCCGCGTGGTCGCGGTCGATTACGGCGCCAAGCGCAACATCCTGCGCTGCC
TGGCCAGCGCGGGCTGCGACGTGACGGTCGTGCCGGCCACGGCCACGGCGGACCAGATCCTGGCCCACGCGCCGGACGGC
GTGTTCCTGTCCAACGGCCCGGGCGACCCGGCCGCGACCGCCGAATATGCCGTGCCGGCGATCCGCGGCGTGCTGGAGGC
CGGCAAGCCGGTCTTCGGCATCTGCCTGGGCCACCAGTTGCTGGCGCAGGCGCTGGGCGCGCGCACCTACAAGCTGGCGC
GCGGCCATCGCGGCGCCAACCAGCCGGTCAAGGACCTGGGAACCGGGCGGGTCGAGATCACGAGCCAGAATCACGGCTTC
GCGGTGGACGAATCCAGCCTGCCCGCCGACGTGCGCGTGACCCATACCAGCCTGTTCGACGGCTCGAACGAGGGCATCGC
CTCCGACCGCTATCCGGCCTTCTCGGTCCAGTACCATCCCGAGGCCAGCCCCGGCCCGTCGGACAGCCATTATCTGTTCG
ACCGCTTCGTCGCCCTGATCGACCGCGTCAACGCACCCGTCTGA

Upstream 100 bases:

>100_bases
GAACATTTTTCCCACCGATTTGCCGTCGATCCGGGTGGGAATTTTCTTCCCAGCCGATCGGGACGCTGGTAGGAGATAAT
CCCGGAAGGAAGAATTCGCC

Downstream 100 bases:

>100_bases
AAGCAGCACACCATGCCCAAACGGACAGATATCCGCTCCATCCTGATCATCGGCGCTGGTCCGATCGTCATCGGCCAGGC
GTGCGAATTCGACTATTCCG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPISIETIIERLGGADHAARLTGVGTEAIRKWRQARAIPPKHWTVILRHTGLSLSDLQPDSASDRAETQMPETPLPPATQ
PPEGATAALVLADGTVAWGRGFGAHTPAAGSAIGELCFSTGMTGYQETLTDPSFAGQIITFTFPHIGNVGTNADDDEAPR
VAARGLAVKQDLTEPANWRATQGLDAWLAGQGVPGICGVDTRAITLRVRDGGPQTAILAYPADGVFDLDALRAQAAAWPG
LEGMDLARDVTCAAPYSWDKGVWTWPAGTCPLPERRRRVVAVDYGAKRNILRCLASAGCDVTVVPATATADQILAHAPDG
VFLSNGPGDPAATAEYAVPAIRGVLEAGKPVFGICLGHQLLAQALGARTYKLARGHRGANQPVKDLGTGRVEITSQNHGF
AVDESSLPADVRVTHTSLFDGSNEGIASDRYPAFSVQYHPEASPGPSDSHYLFDRFVALIDRVNAPV

Sequences:

>Translated_467_residues
MPISIETIIERLGGADHAARLTGVGTEAIRKWRQARAIPPKHWTVILRHTGLSLSDLQPDSASDRAETQMPETPLPPATQ
PPEGATAALVLADGTVAWGRGFGAHTPAAGSAIGELCFSTGMTGYQETLTDPSFAGQIITFTFPHIGNVGTNADDDEAPR
VAARGLAVKQDLTEPANWRATQGLDAWLAGQGVPGICGVDTRAITLRVRDGGPQTAILAYPADGVFDLDALRAQAAAWPG
LEGMDLARDVTCAAPYSWDKGVWTWPAGTCPLPERRRRVVAVDYGAKRNILRCLASAGCDVTVVPATATADQILAHAPDG
VFLSNGPGDPAATAEYAVPAIRGVLEAGKPVFGICLGHQLLAQALGARTYKLARGHRGANQPVKDLGTGRVEITSQNHGF
AVDESSLPADVRVTHTSLFDGSNEGIASDRYPAFSVQYHPEASPGPSDSHYLFDRFVALIDRVNAPV
>Mature_466_residues
PISIETIIERLGGADHAARLTGVGTEAIRKWRQARAIPPKHWTVILRHTGLSLSDLQPDSASDRAETQMPETPLPPATQP
PEGATAALVLADGTVAWGRGFGAHTPAAGSAIGELCFSTGMTGYQETLTDPSFAGQIITFTFPHIGNVGTNADDDEAPRV
AARGLAVKQDLTEPANWRATQGLDAWLAGQGVPGICGVDTRAITLRVRDGGPQTAILAYPADGVFDLDALRAQAAAWPGL
EGMDLARDVTCAAPYSWDKGVWTWPAGTCPLPERRRRVVAVDYGAKRNILRCLASAGCDVTVVPATATADQILAHAPDGV
FLSNGPGDPAATAEYAVPAIRGVLEAGKPVFGICLGHQLLAQALGARTYKLARGHRGANQPVKDLGTGRVEITSQNHGFA
VDESSLPADVRVTHTSLFDGSNEGIASDRYPAFSVQYHPEASPGPSDSHYLFDRFVALIDRVNAPV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=389, Percent_Identity=41.9023136246787, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI169790915, Length=395, Percent_Identity=35.6962025316456, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI21361331, Length=395, Percent_Identity=35.6962025316456, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1786215, Length=385, Percent_Identity=51.6883116883117, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI193204318, Length=382, Percent_Identity=39.5287958115183, Blast_Score=257, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=424, Percent_Identity=36.5566037735849, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=393, Percent_Identity=37.4045801526718, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322638, Length=162, Percent_Identity=31.4814814814815, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=39.7435897435897, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=39.7435897435897, Blast_Score=246, Evalue=4e-65,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 49219; Mature: 49088

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISIETIIERLGGADHAARLTGVGTEAIRKWRQARAIPPKHWTVILRHTGLSLSDLQPD
CCCCHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHCCCCC
SASDRAETQMPETPLPPATQPPEGATAALVLADGTVAWGRGFGAHTPAAGSAIGELCFST
CCCCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHC
GMTGYQETLTDPSFAGQIITFTFPHIGNVGTNADDDEAPRVAARGLAVKQDLTEPANWRA
CCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCC
TQGLDAWLAGQGVPGICGVDTRAITLRVRDGGPQTAILAYPADGVFDLDALRAQAAAWPG
CCCCCHHHCCCCCCCEECCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCC
LEGMDLARDVTCAAPYSWDKGVWTWPAGTCPLPERRRRVVAVDYGAKRNILRCLASAGCD
CCCCCHHHCCEECCCCCCCCCCEECCCCCCCCHHHCCEEEEEECCCHHHHHHHHHHCCCC
VTVVPATATADQILAHAPDGVFLSNGPGDPAATAEYAVPAIRGVLEAGKPVFGICLGHQL
EEEEECCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LAQALGARTYKLARGHRGANQPVKDLGTGRVEITSQNHGFAVDESSLPADVRVTHTSLFD
HHHHHCCHHEEHHHCCCCCCCCHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEEEEEEC
GSNEGIASDRYPAFSVQYHPEASPGPSDSHYLFDRFVALIDRVNAPV
CCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PISIETIIERLGGADHAARLTGVGTEAIRKWRQARAIPPKHWTVILRHTGLSLSDLQPD
CCCHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHCCCCC
SASDRAETQMPETPLPPATQPPEGATAALVLADGTVAWGRGFGAHTPAAGSAIGELCFST
CCCCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHC
GMTGYQETLTDPSFAGQIITFTFPHIGNVGTNADDDEAPRVAARGLAVKQDLTEPANWRA
CCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCC
TQGLDAWLAGQGVPGICGVDTRAITLRVRDGGPQTAILAYPADGVFDLDALRAQAAAWPG
CCCCCHHHCCCCCCCEECCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCC
LEGMDLARDVTCAAPYSWDKGVWTWPAGTCPLPERRRRVVAVDYGAKRNILRCLASAGCD
CCCCCHHHCCEECCCCCCCCCCEECCCCCCCCHHHCCEEEEEECCCHHHHHHHHHHCCCC
VTVVPATATADQILAHAPDGVFLSNGPGDPAATAEYAVPAIRGVLEAGKPVFGICLGHQL
EEEEECCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LAQALGARTYKLARGHRGANQPVKDLGTGRVEITSQNHGFAVDESSLPADVRVTHTSLFD
HHHHHCCHHEEHHHCCCCCCCCHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEEEEEEC
GSNEGIASDRYPAFSVQYHPEASPGPSDSHYLFDRFVALIDRVNAPV
CCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA