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The map label for this gene is ksgA [H]

Identifier: 162149099

GI number: 162149099

Start: 3422188

End: 3423015

Strand: Direct

Name: ksgA [H]

Synonym: GDI_3329

Alternate gene names: 162149099

Gene position: 3422188-3423015 (Clockwise)

Preceding gene: 162149098

Following gene: 162149108

Centisome position: 86.77

GC content: 73.67

Gene sequence:

>828_bases
TTGCTGGAACCGCTGCGCGACGTGATCGCCCGCCACGGGCTGGATGCGCGCAAGGCCCTGGGCCAGCACTTCCTGCTGGA
CCCCGGCATCACCGCACGCATCGCCGCCCTGGCCGGGGACCTGACGGGCCGGCATGTCGTCGAGGTCGGCCCCGGCCCCG
GCGGCCTGACCCGTGCCCTGCTGGACAGCCCGGCCGAGACGGTCACGGCGGTGGAAGTCGATGCGCGCGCGGTCGCGATC
ATCGCCGAACTGGCCACCCTGTTCCCGGGCCGCCTGCACCTGGTCGAGGCCGACGCGACGCGCCAGGACCTGACGCGGCT
CTGCCCCGCCCCGCGCCAGGTCGTCGCCAACCTGCCCTATAATGTCGGCACGCCGCTGCTGGTCGGCTGGCTGCGCCAGG
CATCGGCCTGGGAACGGCTGACCCTGATGTTCCAGATGGAAGTCGCCGAGCGGATCTGCGCCGCCCCCGACAGCGCCCAG
TACGGCCGCCTGGCCGTGCTGGCGCAATGGACCTGCCGGTGCGCCCTGGTCATGCGCATCCCCCCCGGCGCGTTTTCGCC
GCCGCCCAAGGTCTATTCCGCCGTCGTCAGCCTGATCCCCCACGCGACCCAGCCCGACCCCGCCCTGTTCCGCGCCATGG
AACAGGTCACGGCGGCGGCGTTCGGCCAGCGGCGCAAGATGCTGCGCGGCTCCCTGCGCCCGATCGGCGGCGAAGCCCTG
CTGGCCGCCGCCGGCATCGACGGCGCCCGCCGGGCGGAAACGCTGGATATCGCGGAGTTCGACCTTCTGGCGCGGTGCCA
CGCGGAAAGGCAGGGGCTCCCCCCCTGA

Upstream 100 bases:

>100_bases
CAGCTGGATCGCGACCTGCATCGCCGCGCCATCATCGAAATGCGCTCGAAGGTATGAGGCAGCCGCTCTGACCACTCCAC
CCCTGCCCGGTAGCCAGCCG

Downstream 100 bases:

>100_bases
CCCGCCAAAGGGCTCGGCCCTTTGGAAACCCGGTCGTTTTTCTTCCTACCCTGCCCTGGCCCGGCTACGCACGCGCGGGC
GGCGCGTCGCCGCCGCGTCG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLEPLRDVIARHGLDARKALGQHFLLDPGITARIAALAGDLTGRHVVEVGPGPGGLTRALLDSPAETVTAVEVDARAVAI
IAELATLFPGRLHLVEADATRQDLTRLCPAPRQVVANLPYNVGTPLLVGWLRQASAWERLTLMFQMEVAERICAAPDSAQ
YGRLAVLAQWTCRCALVMRIPPGAFSPPPKVYSAVVSLIPHATQPDPALFRAMEQVTAAAFGQRRKMLRGSLRPIGGEAL
LAAAGIDGARRAETLDIAEFDLLARCHAERQGLPP

Sequences:

>Translated_275_residues
MLEPLRDVIARHGLDARKALGQHFLLDPGITARIAALAGDLTGRHVVEVGPGPGGLTRALLDSPAETVTAVEVDARAVAI
IAELATLFPGRLHLVEADATRQDLTRLCPAPRQVVANLPYNVGTPLLVGWLRQASAWERLTLMFQMEVAERICAAPDSAQ
YGRLAVLAQWTCRCALVMRIPPGAFSPPPKVYSAVVSLIPHATQPDPALFRAMEQVTAAAFGQRRKMLRGSLRPIGGEAL
LAAAGIDGARRAETLDIAEFDLLARCHAERQGLPP
>Mature_275_residues
MLEPLRDVIARHGLDARKALGQHFLLDPGITARIAALAGDLTGRHVVEVGPGPGGLTRALLDSPAETVTAVEVDARAVAI
IAELATLFPGRLHLVEADATRQDLTRLCPAPRQVVANLPYNVGTPLLVGWLRQASAWERLTLMFQMEVAERICAAPDSAQ
YGRLAVLAQWTCRCALVMRIPPGAFSPPPKVYSAVVSLIPHATQPDPALFRAMEQVTAAAFGQRRKMLRGSLRPIGGEAL
LAAAGIDGARRAETLDIAEFDLLARCHAERQGLPP

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=294, Percent_Identity=35.3741496598639, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=32.4675324675325, Blast_Score=104, Evalue=9e-23,
Organism=Escherichia coli, GI1786236, Length=259, Percent_Identity=37.8378378378378, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25141369, Length=299, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25146882, Length=215, Percent_Identity=33.0232558139535, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=181, Percent_Identity=33.1491712707182, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=32.6388888888889, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21358017, Length=227, Percent_Identity=31.2775330396476, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 29469; Mature: 29469

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPLRDVIARHGLDARKALGQHFLLDPGITARIAALAGDLTGRHVVEVGPGPGGLTRAL
CCCHHHHHHHHCCCHHHHHHCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
LDSPAETVTAVEVDARAVAIIAELATLFPGRLHLVEADATRQDLTRLCPAPRQVVANLPY
HCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCC
NVGTPLLVGWLRQASAWERLTLMFQMEVAERICAAPDSAQYGRLAVLAQWTCRCALVMRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEC
PPGAFSPPPKVYSAVVSLIPHATQPDPALFRAMEQVTAAAFGQRRKMLRGSLRPIGGEAL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAAAGIDGARRAETLDIAEFDLLARCHAERQGLPP
EEECCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLEPLRDVIARHGLDARKALGQHFLLDPGITARIAALAGDLTGRHVVEVGPGPGGLTRAL
CCCHHHHHHHHCCCHHHHHHCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
LDSPAETVTAVEVDARAVAIIAELATLFPGRLHLVEADATRQDLTRLCPAPRQVVANLPY
HCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCC
NVGTPLLVGWLRQASAWERLTLMFQMEVAERICAAPDSAQYGRLAVLAQWTCRCALVMRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEC
PPGAFSPPPKVYSAVVSLIPHATQPDPALFRAMEQVTAAAFGQRRKMLRGSLRPIGGEAL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAAAGIDGARRAETLDIAEFDLLARCHAERQGLPP
EEECCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA