Click here to switch to the map view.

The map label for this gene is pucA [H]

Identifier: 162146766

GI number: 162146766

Start: 958510

End: 959583

Strand: Reverse

Name: pucA [H]

Synonym: GDI_0947

Alternate gene names: 162146766

Gene position: 959583-958510 (Counterclockwise)

Preceding gene: 162146769

Following gene: 162146765

Centisome position: 24.33

GC content: 68.34

Gene sequence:

>1074_bases
ATGACACCGCACCCTCTTTCCCGCGACCCGCTGGAACTGGCCATTGCCTGGGCCGAGGCTGGGCAGCCCGCCGCCCTGGC
CACCGTCATCGCAACCTGGGGCAGTTCTCCGCGTCCGTCCGGCAGCATGATGGCGGTCAGCGACACGGGCCGGATCGAGG
GATCGGTCAGCGGAGGATGTGTCGAGGCGTCGGTCGTCGACAGCGCCGCGACGATCATGCGTGAAAACACCTCCGAAATC
CTGTCCTACGGCATCACGTCGGAACAGGCATGGTCGGTGGGCCTGGCCTGCGGGGGTGAACTGGACATCCTGGTGGAATC
GGTCGGACCGGCGGGAACGCTGACACTGGCCATGGCCCAACAGGCGATCGCCCTGCGCCGGGCCCGGCAGCCGGTCCTGC
TGGCGACGCGCCTGTCCGACCGGCATCATGTACTGGTCACGCGAACGGAGCAGGGCCTGGAAACCCCGATCAATGACCCG
CCGCCCGACGCCCTGCGCCCGGCGCTGGAGCAGGCGTTTCGCGACGGACGCAGCCAGCGCGTCGAGGATGCGGACGGCGC
GACCTGGTTCCTGCATTTGCTGACACCGCCCCATCGGCTGCTGATCGTCGGCGCCGTGCATATCGCGCAGGTCCTGGCGC
CGCTGGCGATGGCGACCGGTTTCGCAACGACGGTCATCGACCCGCGCACGCAACTGGCCACCCCCGAGCGCTTTCCCGGC
ATTGTCCTGATGACCGACTGGCCCGACGAGGCGCTGGAGAATGTGAACATCGACGATCATACCGCGATCGTCACCCTGAC
CCATGACCCCAAGCTGGACGATCCGACCCTGCACGCCGCGCTGAACAGCCCGGCCTTCTATATCGGCGCGTTGGGGTCAC
GAAAGACCCAGGCCTCGCGCATGGCGCGGCTGCGGGCCGAAGGATTCGACACCGACCGGACCGCCCGCATCCACGGACCG
ATCGGACTGGCCATCGGCGCGGTCGGCGCTCCCGAAATCGCATTGTCGATCCTGGCGGAAATCGTGGCTGTCCGGCGTGA
CGCGCCGCTGGCGCGCGATTCCGGCTGGGGATGA

Upstream 100 bases:

>100_bases
TTGGGGCATGGACCGCATGCCCATCCATCGCGATCTGTACGAGCCGACGGCGCGCCGGGCAAGCTGGCCTGACTATATCC
ACGCTGATAGAGTGATCGGC

Downstream 100 bases:

>100_bases
GCACCGGGGTGGCTACCGGAATCGTGGCGCTGGTCCTGGCTGCGGGCCGGTCATCGCGATCGGGGGACCGCCATAAATTG
CTGGCCCCCGACCAGATGGG

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTPHPLSRDPLELAIAWAEAGQPAALATVIATWGSSPRPSGSMMAVSDTGRIEGSVSGGCVEASVVDSAATIMRENTSEI
LSYGITSEQAWSVGLACGGELDILVESVGPAGTLTLAMAQQAIALRRARQPVLLATRLSDRHHVLVTRTEQGLETPINDP
PPDALRPALEQAFRDGRSQRVEDADGATWFLHLLTPPHRLLIVGAVHIAQVLAPLAMATGFATTVIDPRTQLATPERFPG
IVLMTDWPDEALENVNIDDHTAIVTLTHDPKLDDPTLHAALNSPAFYIGALGSRKTQASRMARLRAEGFDTDRTARIHGP
IGLAIGAVGAPEIALSILAEIVAVRRDAPLARDSGWG

Sequences:

>Translated_357_residues
MTPHPLSRDPLELAIAWAEAGQPAALATVIATWGSSPRPSGSMMAVSDTGRIEGSVSGGCVEASVVDSAATIMRENTSEI
LSYGITSEQAWSVGLACGGELDILVESVGPAGTLTLAMAQQAIALRRARQPVLLATRLSDRHHVLVTRTEQGLETPINDP
PPDALRPALEQAFRDGRSQRVEDADGATWFLHLLTPPHRLLIVGAVHIAQVLAPLAMATGFATTVIDPRTQLATPERFPG
IVLMTDWPDEALENVNIDDHTAIVTLTHDPKLDDPTLHAALNSPAFYIGALGSRKTQASRMARLRAEGFDTDRTARIHGP
IGLAIGAVGAPEIALSILAEIVAVRRDAPLARDSGWG
>Mature_356_residues
TPHPLSRDPLELAIAWAEAGQPAALATVIATWGSSPRPSGSMMAVSDTGRIEGSVSGGCVEASVVDSAATIMRENTSEIL
SYGITSEQAWSVGLACGGELDILVESVGPAGTLTLAMAQQAIALRRARQPVLLATRLSDRHHVLVTRTEQGLETPINDPP
PDALRPALEQAFRDGRSQRVEDADGATWFLHLLTPPHRLLIVGAVHIAQVLAPLAMATGFATTVIDPRTQLATPERFPGI
VLMTDWPDEALENVNIDDHTAIVTLTHDPKLDDPTLHAALNSPAFYIGALGSRKTQASRMARLRAEGFDTDRTARIHGPI
GLAIGAVGAPEIALSILAEIVAVRRDAPLARDSGWG

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786477, Length=339, Percent_Identity=31.858407079646, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789240, Length=179, Percent_Identity=30.7262569832402, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 37818; Mature: 37687

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPHPLSRDPLELAIAWAEAGQPAALATVIATWGSSPRPSGSMMAVSDTGRIEGSVSGGC
CCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCE
VEASVVDSAATIMRENTSEILSYGITSEQAWSVGLACGGELDILVESVGPAGTLTLAMAQ
EEHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHH
QAIALRRARQPVLLATRLSDRHHVLVTRTEQGLETPINDPPPDALRPALEQAFRDGRSQR
HHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
VEDADGATWFLHLLTPPHRLLIVGAVHIAQVLAPLAMATGFATTVIDPRTQLATPERFPG
CCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCC
IVLMTDWPDEALENVNIDDHTAIVTLTHDPKLDDPTLHAALNSPAFYIGALGSRKTQASR
EEEEECCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHECCCCEEEEECCCCCHHHHHH
MARLRAEGFDTDRTARIHGPIGLAIGAVGAPEIALSILAEIVAVRRDAPLARDSGWG
HHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TPHPLSRDPLELAIAWAEAGQPAALATVIATWGSSPRPSGSMMAVSDTGRIEGSVSGGC
CCCCCCCCCHHEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCE
VEASVVDSAATIMRENTSEILSYGITSEQAWSVGLACGGELDILVESVGPAGTLTLAMAQ
EEHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHH
QAIALRRARQPVLLATRLSDRHHVLVTRTEQGLETPINDPPPDALRPALEQAFRDGRSQR
HHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
VEDADGATWFLHLLTPPHRLLIVGAVHIAQVLAPLAMATGFATTVIDPRTQLATPERFPG
CCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCC
IVLMTDWPDEALENVNIDDHTAIVTLTHDPKLDDPTLHAALNSPAFYIGALGSRKTQASR
EEEEECCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHECCCCEEEEECCCCCHHHHHH
MARLRAEGFDTDRTARIHGPIGLAIGAVGAPEIALSILAEIVAVRRDAPLARDSGWG
HHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]