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The map label for this gene is dsbA2 [H]

Identifier: 161870770

GI number: 161870770

Start: 1894092

End: 1894787

Strand: Reverse

Name: dsbA2 [H]

Synonym: NMCC_1851

Alternate gene names: 161870770

Gene position: 1894787-1894092 (Counterclockwise)

Preceding gene: 161870771

Following gene: 161870768

Centisome position: 87.99

GC content: 49.57

Gene sequence:

>696_bases
ATGAAACTGAAAACCTTAGCTTTGACTTCATTGACCCTGTTGGCATTGGCCGCTTGTAGCAAACAGGCTGAAACCAGCGT
TCCGGCAGACAGCGCCCAAAGCAACACATCCGCTCCGGCAACCCCTGCCGCATTGACCGAAGGTATCAATTACACCGTAT
TGTCCAATCCGATTCCGCAACAGCAGGCCGGCAAAATCGAAGTATTGGAATTCTTCGGCTACTTCTGCCCGCATTGCGCC
CATCTTGAGCCGGTCTTGAGCGAGCACATCAAAACGTTTAAAGACGATACCTATATGCGCCGGGAGCATGTCGTGTGGGG
TGATGAAATGAAACCTTTGGCACGTTTGGCGGCCGCAGTGGAAATGGCCGGTGAATCAGATAAAGCCAACAGCCATATTT
TCGATGCGATGGTTAATCAAAAAATCAATCTGGCCGATACCGATACCCTGAAAAAATGGCTGTCCGAGCAAACAGCGTTT
GACGGCAAAAAAGTATTGGCTGCATTTGAGGCTCCTGAAAGCCAAGCGCGTGCGGCTCAAATGGAAGAGTTGACCAATAA
ATTCCAAATCAGCGGCACACCGACTGTGATCGTCGGCGGCAAATACCAAGTTGAATTTAAAGACTGGCAGTCCGGTATGA
CCACGATTGACCAGTTGGTGGATAAAGTACGCGAAGAGCAGAAAAAGCCGCAATAA

Upstream 100 bases:

>100_bases
GGCTGAAATAAAACTAAACACATTTTTTCACTCAAATCGAACGCGCCTTCAATAAAATGCCATAAAATCCGCACATTAAT
CTGACACACAAGAGATACCT

Downstream 100 bases:

>100_bases
GTTGAGGATTGAATGAGTAAAGGCCATCTGAAAATAGGATTTCAGACGGCCTTTTGTATTTAGGCTTTATAGAAGAGATG
ATTGCTTAAAGCCTTATGGT

Product: thiol:disulphide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAALTEGINYTVLSNPIPQQQAGKIEVLEFFGYFCPHCA
HLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ

Sequences:

>Translated_231_residues
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAALTEGINYTVLSNPIPQQQAGKIEVLEFFGYFCPHCA
HLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ
>Mature_231_residues
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAALTEGINYTVLSNPIPQQQAGKIEVLEFFGYFCPHCA
HLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=171, Percent_Identity=32.1637426900585, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR013766
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA; PF00085 Thioredoxin [H]

EC number: NA

Molecular weight: Translated: 25447; Mature: 25447

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAALTEGINYTVLSNPIPQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCC
QQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAV
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHH
EMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAFDGKKVLAAFEAPESQARAAQ
HHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
MEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ
HHHHHHHEECCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAALTEGINYTVLSNPIPQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCC
QQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAV
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHH
EMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAFDGKKVLAAFEAPESQARAAQ
HHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
MEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ
HHHHHHHEECCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA