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The map label for this gene is pilF [H]

Identifier: 161870741

GI number: 161870741

Start: 1859741

End: 1861417

Strand: Reverse

Name: pilF [H]

Synonym: NMCC_1814

Alternate gene names: 161870741

Gene position: 1861417-1859741 (Counterclockwise)

Preceding gene: 161870742

Following gene: 161870736

Centisome position: 86.44

GC content: 52.71

Gene sequence:

>1677_bases
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGCCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGACAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Upstream 100 bases:

>100_bases
CTCTTTGAGCTAAGGCGAGGCAACGCCGTACTGGTTTAAATTTAATCCACTATAAAAGCTGTACAGGTATAACAATGAAT
AAATTTGGGGATAAGGTCGT

Downstream 100 bases:

>100_bases
GTTTGAGAATGAAAATGCCGTCTGAAGCGTGTTTGTTTCAGACGGCATTTGATTTTCAGGGGTTTGTCGGAGGACGGGGT
GCGGTCAGCGGTATGCGCCG

Product: type IV pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Sequences:

>Translated_558_residues
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND
>Mature_557_residues
SVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQM
VEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTLY
IDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKRI
PQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGK
TVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLYR
AVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=389, Percent_Identity=45.5012853470437, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1786296, Length=406, Percent_Identity=43.5960591133005, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=34.3589743589744, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 61966; Mature: 61835

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSI
CCCHHEEHHHHCCEEEHHHHHHHHCHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEV
HHHHHCCCHHHHHHHHCHHHHHEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCEEE
ELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTLYIDNEEAEDGPVPRFIHKTL
EEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
SDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
HHHHHCCCCCEEEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLL
CCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHH
LEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVND
HHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCEECCCCCCEECCCC
KQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNNAPATLSR
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHH
MLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGIL
HHHCCHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHH
KVMQGITSLEEVTANTND
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSI
CCHHEEHHHHCCEEEHHHHHHHHCHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEV
HHHHHCCCHHHHHHHHCHHHHHEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCEEE
ELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTLYIDNEEAEDGPVPRFIHKTL
EEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
SDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
HHHHHCCCCCEEEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLL
CCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHH
LEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVND
HHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCEECCCCCCEECCCC
KQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNNAPATLSR
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHH
MLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGIL
HHHCCHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHH
KVMQGITSLEEVTANTND
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]