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The map label for this gene is dusA [H]

Identifier: 161870721

GI number: 161870721

Start: 1841538

End: 1842548

Strand: Reverse

Name: dusA [H]

Synonym: NMCC_1794

Alternate gene names: 161870721

Gene position: 1842548-1841538 (Counterclockwise)

Preceding gene: 161870722

Following gene: 161870717

Centisome position: 85.56

GC content: 58.16

Gene sequence:

>1011_bases
ATGAACGACAACACGCAAACCCTTCCCCCACGCCGCCTGTCCGTCGCCCCGATGCTCGACTGGACGGACAGGCACTACCG
TTATATGGCGCGCCAGATTACCCGAAATACTTGGCTTTACAGCGAAATGGTCAATGCCGGTGCGATTGTTTACGGCGACA
AAGACCGCTTTTTGATGTTTAACGAAGGCGAGCAGCCCGTCGCCCTGCAACTGGGCGGCAGCGATCCGTCCGATTTGGCG
AAAGCCGCCAAAGCTGCCGAAGCATACGGCTACAACGAGGTCAACCTCAACTGCGGCTGCCCCAGTCCGCGCGTGCAGAA
AGGCGCGTTCGGCGCGTGTCTGATGAACGAAGTCGGGCTGGTTGCCGACTGCCTCAACGCGATGCAGGACGCGGTCGGCA
TTCCCGTTACCGTCAAACACCGCATCGGTGTGGACAGGCAGACCGAATACCAAACCGTTGCCGATTTCGTCGGCACGCTG
CGTGACAAAACCGCCTGCAAAACCTTTATCGTCCACGCCCGCAACGCATGGCTGGACGGACTTTCCCCCAAAGAAAACCG
CGATGTTCCGCCCTTGAAATACGATTACGTTTACCGTCTTAAGCAGGAGTTTCCCGAGCTGGAAATCATCATCAACGGCG
GCGTCACCACCAACGAAGCAATCGCCGGACACCTGCAACACGTTGACGGCGTGATGGTCGGGCGCGAGGCGTACCACAAC
CCGATGGTGATGCGCGAATGGGACAGGCTGTTTTACGGCGATACCCGCAGCCCGATTGAATACGCCGATTTGGTGCAGCG
TCTCTACACATACAGCCAAGCCCAAATCCAAGCCGGACGCGGCACAATCTTGCGTCACATCGTCCGCCACAGCCTTGGGC
TGATGCACGGTCTGAAAGGCGCGCGGACTTGGCGGCGTATGCTTTCCGACGCAACGCTCTTGAAAGACAACGACGGCAGC
CTGATTCTCGAAGCATGGAAAGAGGTTGAGTGGGCAAATACGCGTGAATAG

Upstream 100 bases:

>100_bases
GCCGGTAACCATACTGCTGGACTCTTTCATGACGCGGATTTCCCCAAAAATGAAGGTTGTTCCGGATTGAAATTGAATCC
GCAATGGTGAAATATCGACA

Downstream 100 bases:

>100_bases
GGCGGGGCTGTATGTGTGAAATGCCGTCTGAAGGTTTCAGACGGCATTTGTGCGTTTGTCGGGCGGTGTTCAGGGGGGGG
TAACGGCGTGTTTCGGCACT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLA
KAAKAAEAYGYNEVNLNCGCPSPRVQKGAFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGVTTNEAIAGHLQHVDGVMVGREAYHN
PMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGS
LILEAWKEVEWANTRE

Sequences:

>Translated_336_residues
MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLA
KAAKAAEAYGYNEVNLNCGCPSPRVQKGAFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGVTTNEAIAGHLQHVDGVMVGREAYHN
PMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGS
LILEAWKEVEWANTRE
>Mature_336_residues
MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLA
KAAKAAEAYGYNEVNLNCGCPSPRVQKGAFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGVTTNEAIAGHLQHVDGVMVGREAYHN
PMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGS
LILEAWKEVEWANTRE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=255, Percent_Identity=27.0588235294118, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI40807366, Length=280, Percent_Identity=24.6428571428571, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=54.4025157232704, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1789660, Length=264, Percent_Identity=27.6515151515151, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788462, Length=229, Percent_Identity=27.9475982532751, Blast_Score=68, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=296, Percent_Identity=25, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24580595, Length=290, Percent_Identity=23.7931034482759, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920448, Length=290, Percent_Identity=23.7931034482759, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37995; Mature: 37995

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMVNAGAIVYGDKDRFLMF
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
NEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPSPRVQKGAFGACLMNEVGL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
VADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTLRDKTACKTFIVHARNAWLDG
HHHHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGVTTNEAIAGHLQHVDGVMVGREAYHN
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCC
PMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKG
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ARTWRRMLSDATLLKDNDGSLILEAWKEVEWANTRE
HHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMVNAGAIVYGDKDRFLMF
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
NEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPSPRVQKGAFGACLMNEVGL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
VADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTLRDKTACKTFIVHARNAWLDG
HHHHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGVTTNEAIAGHLQHVDGVMVGREAYHN
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCC
PMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKG
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ARTWRRMLSDATLLKDNDGSLILEAWKEVEWANTRE
HHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]