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The map label for this gene is prpB [H]

Identifier: 161870645

GI number: 161870645

Start: 1757105

End: 1757980

Strand: Reverse

Name: prpB [H]

Synonym: NMCC_1715

Alternate gene names: 161870645

Gene position: 1757980-1757105 (Counterclockwise)

Preceding gene: 161870646

Following gene: 161870644

Centisome position: 81.64

GC content: 53.42

Gene sequence:

>876_bases
ATGAGTCAACACTCTGCCGGAGCACGTTTCCGCCAAGCCGTGAAAGAATCGAATCCGCTTGCCGTCGCCGGTTGCGTCAA
TGCTTATTTTGCACGATTGGCCACCCAAAGCGGTTTCAAAGCCATCTATCTGTCCGGCGGCGGCGTGGCAGCCTGTTCTT
GCGGTATCCCTGATTTGGGCATTACCACAATGGAAGATGTGCTGATCGACGCACGACGCATTACGGACAACGTGGATACG
CCTCTGCTGGTGGACATCGATGTGGGTTGGGGCGGTGCATTCAATATTGCCCGTACCATTCGCAACTTTGAACGCGCCGG
TGTTGCAGCGGTTCACATCGAAGATCAGGTAGCGCAAAAACGCTGCGGCCACCGTCCGAACAAAGCCATTGTATCTAAAG
ATGAAATGGTCGACCGTATCAAAGCTGCCGTAGATGCGCGCGTTGATGAGAACTTCGTGATTATGGCGCGTACCGATGCG
CTGGCGGTAGAAGGTTTGGATGCCGCTATCGAACGCGCCCAAGCTTGTGTCGAAGCCGGTGCGGACATGATTTTCCCTGA
AGCCATGACCGATTTGAACATGTACCGCCAATTTGCAGATGTGGTGAAAGTGCCCGTGTTGGCGAACATTACCGAGTTTG
GTTCCACTCCGCTTTATACCCAAAGCGAGCTGGCTGAAAACGGCGTGTCGCTGGTGCTGTATCCGCTGTCATCGTTCCGT
GCAGCAAGCAAAGCCGCTCTGAATGTTTACGAAGCGATTATGCGCGATGGCACTCAGGCGGCGGTGGTGGACAGTATGCA
AACCCGTGCCGAGCTGTACGAGCATCTGAACTATCATGCCTTCGAGCAAAAACTGGATAAATTGTTTCAAAAATGA

Upstream 100 bases:

>100_bases
TTGGATTGGGATGCCCGACGCGTCGGATGGCTGTGTTTTGCCGTCCGAATGTGATGGAAGCCTGTCCATACTGAAAAAAA
GTCTATAAAGGAGAAATATG

Downstream 100 bases:

>100_bases
TTTACCGCTTTCAGACGGTCTTTCAACAAATCCGCATCGGTCGTCTGAAAACCCGAAACCCATAAAAACACAAAGGAGAA
ATACCATGACTGAAACTACT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYLSGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDT
PLLVDIDVGWGGAFNIARTIRNFERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPLYTQSELAENGVSLVLYPLSSFR
AASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHLNYHAFEQKLDKLFQK

Sequences:

>Translated_291_residues
MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYLSGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDT
PLLVDIDVGWGGAFNIARTIRNFERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPLYTQSELAENGVSLVLYPLSSFR
AASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHLNYHAFEQKLDKLFQK
>Mature_290_residues
SQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYLSGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTP
LLVDIDVGWGGAFNIARTIRNFERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDAL
AVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPLYTQSELAENGVSLVLYPLSSFRA
ASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHLNYHAFEQKLDKLFQK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=64.7260273972603, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31611; Mature: 31480

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYLSGGGVAACSCGIPDLG
CCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCC
ITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRNFERAGVAAVHIEDQVAQK
CHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHH
RCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDALAVEGLDAAIERAQACVEAG
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCC
ADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPLYTQSELAENGVSLVLYPLSSFR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECHHHH
AASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHLNYHAFEQKLDKLFQK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYLSGGGVAACSCGIPDLG
CCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCC
ITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRNFERAGVAAVHIEDQVAQK
CHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHH
RCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDALAVEGLDAAIERAQACVEAG
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCC
ADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPLYTQSELAENGVSLVLYPLSSFR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECHHHH
AASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHLNYHAFEQKLDKLFQK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]