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The map label for this gene is rlmL [H]

Identifier: 161870620

GI number: 161870620

Start: 1726910

End: 1728052

Strand: Reverse

Name: rlmL [H]

Synonym: NMCC_1689

Alternate gene names: 161870620

Gene position: 1728052-1726910 (Counterclockwise)

Preceding gene: 161870621

Following gene: 161870616

Centisome position: 80.25

GC content: 56.69

Gene sequence:

>1143_bases
ATGAATACTTTATATACACTTTTCGCCACCTGCCCGCGCGGCTTGGAAACCGTTTTATCGCAAGAACTCGAAAGCCTCGG
CTGTACCGATGTACAAGTGTTTGACGGCGGCGTTTCCTGCCGGGGCGGATTGGAACAGGTTTACGCCGCCAACCTGCATT
CGCGTACTGCCAGCCGTATCCTGCTGCGCCTGACCAAAGGGACATACCGCAATGAGCGCGACATCTACAAACTCGCCAAA
AATATCAACTGGTTTAATTGGTTTACTTTACAGCAGACGTTCAAAGTCAAAGTCGAGGCAAAGCGTGCCAACGTTAAGAG
CATCCAATTTGTCGGACTGACCGTCAAAGATGCCGTCTGCGACGCTTTCCGCGACATTTACGACGCACGTCCGAGCGTGG
ACAAAGCCGCGCCCGATGTTCGCATCCACGCCTTTTTGGACGAACGCAATGTCGAAATCTTCATCGACACTTCGGGCGAA
GCCCTGTTCAAACGCGGCTACCGCCTGGATACCGGCGAAGCCCCGCTGCGCGAAAACCTTGCCGCCGGACTGCTGCTCTC
GGCAGGCTACGACGGCACGCAGCCGTTTCAAGACCCGTTTTGCGGCAGCGGCACGATTGCTATCGAAGCCGCTTGGATTG
CCGCCCGCCGCGCACCGGGTATGATGCGCCGCTTCGGTTTTGAAAAACTGCAAAATTTCGATAAAACGCTGTGGGCGGAT
TTGCGCCGCCGCGCCGAAGCGCAGGTCAACCCCGCTTTTGCACCGATTTCGGGCAGCGACAACGACCGCCGCATCGTTCA
GACGGCATTGGACAACGCACGCAGTGCCGAAGTGGATGACATCGTTTCCTTCAGCGTTGCCGACGCGCAATCCGTCCGTC
CGAACGGCGAAAACGGCATCATGGTGTCCAATCCGCCCTACGGTGTGCGCCTTGAGGAAGTCCGCGCTTTGCAGGCACTT
TATCCGCAGTTGGGGACGTGGTTGAAAAAACACTATGCCGGCTGGCGGGTCGGCATGTTTACCAGCGACAGGGAAATGCC
CAAATTCATGCGCCTGTCGCCCAAGCGGAAAATTCCGCTTTATAACGGCAACCTCGACTGCCGCCTGTTCCTGATTGATA
TGGTGCAGGGATCGAACCGTTGA

Upstream 100 bases:

>100_bases
GGACATTACAATCGGCAGCCGGACATTTCAGACGGCATTTACCGCCGAATAAACCTTTCAATCCGCCATTGCCGGAACAT
CCGTCCGGAAAGGACACGCT

Downstream 100 bases:

>100_bases
GGAAAGTGTACAAAAAAGCCGTCTGAAAAATGTTCAGACGGCTTTTTTTAATATCCGGCCGATATTATAGTGGATTAACA
AAAATCAGGACAAGGCGACG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGLEQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAK
NINWFNWFTLQQTFKVKVEAKRANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRAPGMMRRFGFEKLQNFDKTLWAD
LRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSAEVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQAL
YPQLGTWLKKHYAGWRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR

Sequences:

>Translated_380_residues
MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGLEQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAK
NINWFNWFTLQQTFKVKVEAKRANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRAPGMMRRFGFEKLQNFDKTLWAD
LRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSAEVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQAL
YPQLGTWLKKHYAGWRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR
>Mature_380_residues
MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGLEQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAK
NINWFNWFTLQQTFKVKVEAKRANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRAPGMMRRFGFEKLQNFDKTLWAD
LRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSAEVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQAL
YPQLGTWLKKHYAGWRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=372, Percent_Identity=37.3655913978495, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 42726; Mature: 42726

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGLEQVYAANLHSRTASRI
CCCEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKRANVKSIQFVGLTVKDAVC
HHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEEEEEEEHHHHHH
DAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGEALFKRGYRLDTGEAPLRENL
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHCCCCCCCCCCCHHHHH
AAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRAPGMMRRFGFEKLQNFDKTLWAD
HHHEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHCHHHHHHHHHHHHHH
LRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSAEVDDIVSFSVADAQSVRPNGENGI
HHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCHHHHHEEECCCHHHCCCCCCCCE
MVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAGWRVGMFTSDREMPKFMRLSPKRKIPL
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCE
YNGNLDCRLFLIDMVQGSNR
ECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGLEQVYAANLHSRTASRI
CCCEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKRANVKSIQFVGLTVKDAVC
HHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEEEEEEEHHHHHH
DAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGEALFKRGYRLDTGEAPLRENL
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHCCCCCCCCCCCHHHHH
AAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRAPGMMRRFGFEKLQNFDKTLWAD
HHHEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHCHHHHHHHHHHHHHH
LRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSAEVDDIVSFSVADAQSVRPNGENGI
HHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCHHHHHEEECCCHHHCCCCCCCCE
MVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAGWRVGMFTSDREMPKFMRLSPKRKIPL
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCE
YNGNLDCRLFLIDMVQGSNR
ECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA