Click here to switch to the map view.

The map label for this gene is dusB [H]

Identifier: 161870286

GI number: 161870286

Start: 1333715

End: 1334839

Strand: Reverse

Name: dusB [H]

Synonym: NMCC_1327

Alternate gene names: 161870286

Gene position: 1334839-1333715 (Counterclockwise)

Preceding gene: 161870289

Following gene: 161870285

Centisome position: 61.99

GC content: 58.22

Gene sequence:

>1125_bases
GTGTGCGGATTATGCCATAAAACGGTGCCCGCCCCAATCGCGGGCGCGTCCGGAGATTGGAAATCCTGCTTAAAAAATGT
ACAATGTCGCACTTTTTTGAAACGCGGACCCATTATGCACATCGGCGGCTATTTTATCGACAACCCCATCGCACTTGCGC
CGATGGCGGGCATTACCGACAAACCCTTCCGCCGCCTTTGCCGCGAGTTTGGCGCGGGTTGGGCAGTGTGCGAAATGCTG
ACCAGCGACCCGACGCTCAGAAATACTAGAAAAACCTTGCACCGCAGCGATTTTGTCGATGAAGGCGGCATCGTTGCCGT
GCAGATTGCCGGCAGCGATCCGCAGCAGATGGCGGATGCGGCGCGTTACAACGTCAGCCTCGGGGCGCAGCTTATCGACA
TCAATATGGGCTGTCCCGCCAAAAAAGTGTGCAACGTCCAAGCCGGCAGCGCGCTGATGCAGAACGAGCCGCTGGTTGCC
GCCATTTTGGAAGCCGTCGTCCGTGCGGCAGGCGTACCCGTTACCCTCAAAACCCGTTTGGGCTGGCACGAAGACCATCA
AAACCTGCCCGTCATCGCCAAAATCGCCGAAGATTGCGGCATCGCCGCCCTTGCCGTCCACGGACGCACGCGCACGCAAA
TGTACAAAGGCGAGGCGCGTTACGAACTCATCGCCGAAACCAAATGCCGTCTGAACATCCCGGTCTGGGTCAACGGCGAC
ATCACTTCGCCGCAAAAAGCCGCCGCCGTCCTCAAACAAACCGCCGCCGACGGCATCATGATAGGGCGCGGCGCGCAAGG
CAGACCGTGGTTCTTCCGCGATTTGAAACATTACGCCGAACACGGTGTTTTACCGCCTGCCTTGAGTTTGGCAGAATGCA
GTGCCGCCATTTTGAACCACGTCCGCGCCATGCACGCGTTTTATGGTGAGACCGCCGGTGTGCGCATTGCACGCAAACAC
ATAGGCTGGTACATCGACGAAATGCCCGACGGCGAACAGACACGTCGTGAAATCAACCGCTTGGACAGTGCGGCGGCGCA
ATACGACATGCTTGCAGGTTATCTTGAAAGACTTGCCGGAAAAACCGACCGTTGGGCGTGCGCCTACCGCCCAAATGCCT
TCTGA

Upstream 100 bases:

>100_bases
TAGAGAATCGTTCTCTTTGAGCTAAGGCGAGGCAACGCCGTACTGGTTTTTGTTAATCCACTATAAACTGCCCGCCTGTG
AGTGGCGGGGCAGGGAATCT

Downstream 100 bases:

>100_bases
ACACTTGATTATCCTTTGAAAGTGCAATCATGCCCCATACCCTTCCCGATATTTCCCAATGTATCAGACAAAATTTGGAA
CAATATTTCAAAGACCTGAA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MCGLCHKTVPAPIAGASGDWKSCLKNVQCRTFLKRGPIMHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEML
TSDPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPLVA
AILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAEDCGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGD
ITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGVRIARKH
IGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRPNAF

Sequences:

>Translated_374_residues
MCGLCHKTVPAPIAGASGDWKSCLKNVQCRTFLKRGPIMHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEML
TSDPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPLVA
AILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAEDCGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGD
ITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGVRIARKH
IGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRPNAF
>Mature_374_residues
MCGLCHKTVPAPIAGASGDWKSCLKNVQCRTFLKRGPIMHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEML
TSDPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPLVA
AILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAEDCGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGD
ITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGVRIARKH
IGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRPNAF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=32.7800829875519, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=32.7800829875519, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI31742496, Length=256, Percent_Identity=29.6875, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=31.4285714285714, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI40807366, Length=225, Percent_Identity=28.8888888888889, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=53.8940809968847, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI145693211, Length=241, Percent_Identity=27.3858921161826, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=237, Percent_Identity=29.957805907173, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=28.6885245901639, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=27.7272727272727, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=232, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=27.7056277056277, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=28.8793103448276, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=25.8687258687259, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=286, Percent_Identity=29.3706293706294, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=246, Percent_Identity=30.0813008130081, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920448, Length=246, Percent_Identity=30.0813008130081, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=30.7017543859649, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45549423, Length=257, Percent_Identity=27.6264591439689, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 40876; Mature: 40876

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLCHKTVPAPIAGASGDWKSCLKNVQCRTFLKRGPIMHIGGYFIDNPIALAPMAGITD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCEEECCCEEECCCCCCCC
KPFRRLCREFGAGWAVCEMLTSDPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADA
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
ARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRL
HHCCCEECCEEEEECCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECC
GWHEDHQNLPVIAKIAEDCGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGD
CCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHCCCCCEEEEECCCEEEECCEEECCC
ITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNH
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VRAMHAFYGETAGVRIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAG
HHHHHHHHCCCCCHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KTDRWACAYRPNAF
CCCCEEEECCCCCC
>Mature Secondary Structure
MCGLCHKTVPAPIAGASGDWKSCLKNVQCRTFLKRGPIMHIGGYFIDNPIALAPMAGITD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCEEECCCEEECCCCCCCC
KPFRRLCREFGAGWAVCEMLTSDPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADA
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
ARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRL
HHCCCEECCEEEEECCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECC
GWHEDHQNLPVIAKIAEDCGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGD
CCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHCCCCCEEEEECCCEEEECCEEECCC
ITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNH
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VRAMHAFYGETAGVRIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAG
HHHHHHHHCCCCCHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KTDRWACAYRPNAF
CCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]