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The map label for this gene is msbB [H]

Identifier: 161870283

GI number: 161870283

Start: 1331987

End: 1332886

Strand: Reverse

Name: msbB [H]

Synonym: NMCC_1324

Alternate gene names: 161870283

Gene position: 1332886-1331987 (Counterclockwise)

Preceding gene: 161870284

Following gene: 161870282

Centisome position: 61.9

GC content: 50.11

Gene sequence:

>900_bases
TTGCCGTCTGAAAAGAAAATGTGTATCGAGATGAAATTTATATTTTTTGTACTGTATGTTTTGCAGTTTCTGCCGTTTGC
GCTGCTGCACAAGATTGCCGACCTGACGGGTTTGCTTGCCTACCTTCTGGTCAAACCGCGCCGCCGTATCGGCGAAATCA
ATTTGGCGAAATGTTTTCCCGAATGGAGTGAGGAAAAGCGTAAAACCGTGTTGAAACAGCATTTCAAACATATGGCGAAA
CTGATGTTGGAATACGGTTTATATTGGTACGCGCCTGCCGGGCGTTTGAAATCGCTGGTGCGTTACCGCAATAAGCATTA
TTTGGACGACGCGCTGGCGGTGGGGGAAAAAGTCATCATCCTGTATCCGCACTTCACCGCGTTCGAGATGGCGGTGTACG
CGCTTAATCAGGATGTCCCGCTGATCAGTATGTATTCCCATCAAAAAAACAAGATATTGGACGAACAGATTTTGAAAGGT
CGCAACCGCTATCACAACGTCTTCCTTATCGGGCGCACCGAAGGGCTGCGCGCCCTCGTCAAACAGTTCCGCAAAAGCAG
CGCGCCGTTTCTGTATCTGCCCGATCAGGATTTCGGACGCAACGATTCGGTTTTTGTGGATTTCTTCGGTATTCAGACGG
CAACGATTACCGGATTGAGCCGCATTGCCGCGCTTGCAAATGCAAAAGTGATACCCGCCATTCCCGTCCGCGAGGCAGAC
AATACAGTTACACTGCATTTCTACCCGGCTTGGAAATCCTTTCCGAGTGAAGATGCGCAAGCCGACGCGCAGCGCATGAA
CCGTTTTATCGAGGAACGCGTGCGCGAACATCCCGAGCAGTATTTTTGGCTGCACAAGCGTTTTAAAACCCGTCCGGAAG
GCAGCCCCGATTTTTACTGA

Upstream 100 bases:

>100_bases
CCCACGCCTTACGCAACCACGGGCTTGCGTCCCGGCTCAACCCGTCGGGGATGCAGGTCAAGCGCGGAAGGTTTCAATAG
TTTCAGACGGCATTTGTATT

Downstream 100 bases:

>100_bases
CTACGTAAAATTACAAAACATATCAGGCATTTCGAGTCGAAACTCCTGATTGTTTTTTTAAATGCAAAATGGCAGATTAT
ATGAACAGATTTCATTTGAT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPSEKKMCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFPEWSEEKRKTVLKQHFKHMAK
LMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKG
RNRYHNVFLIGRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREAD
NTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFY

Sequences:

>Translated_299_residues
MPSEKKMCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFPEWSEEKRKTVLKQHFKHMAK
LMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKG
RNRYHNVFLIGRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREAD
NTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFY
>Mature_298_residues
PSEKKMCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFPEWSEEKRKTVLKQHFKHMAKL
MLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGR
NRYHNVFLIGRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREADN
TVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=285, Percent_Identity=31.9298245614035, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI87082094, Length=289, Percent_Identity=30.7958477508651, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788161, Length=261, Percent_Identity=29.8850574712644, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35089; Mature: 34958

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEKKMCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHCC
EWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAVGEKVII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLIGRTEGLRALV
EECCHHHHHHHHHHHCCCCCEEEHHHCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHH
KQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREAD
HHHHHCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFY
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PSEKKMCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHCC
EWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAVGEKVII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLIGRTEGLRALV
EECCHHHHHHHHHHHCCCCCEEEHHHCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHH
KQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREAD
HHHHHCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFY
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]