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The map label for this gene is trmB [H]

Identifier: 161870203

GI number: 161870203

Start: 1235616

End: 1236332

Strand: Direct

Name: trmB [H]

Synonym: NMCC_1243

Alternate gene names: 161870203

Gene position: 1235616-1236332 (Clockwise)

Preceding gene: 161870202

Following gene: 161870208

Centisome position: 57.38

GC content: 55.37

Gene sequence:

>717_bases
ATGACAGACACCGCCGAAAACCAAACACCAAACGACCGTCAAGCCGGACATCCCCGCAGCATCCGCAGTTTCGTCCTCCG
CCAAAGCCATATGACCGCCGCGCAGCAACGCGCCATCGATACCTTATGGGACAGCTTCGGCATCGATTACCAAGCAACAC
CGGTCGATCTTGATGCCAATTTCGGAAGCAACCGCCCCAAAATCCTCGAAATAGGCTTCGGTATGGGGACAGCAACCGCA
GAAATCGCCCTCCGCCTGCCCGAAACCGACTTCCTCGCCATCGACGTACACGGTCCCGGCGTAGGCAACCTGCTCAAACT
CATCAACGAAAACCATTTAGAAAACATCCGCGTGATGCGGCACGATGCCGTAGAAGTTGTCGAAAATATGCTGCAAGACG
GCTCGCTCGACGGCATCCACATATTCTTCCCCGACCCGTGGCACAAAAAACGCCACCACAAACGCCGTCTGATACAAGCC
CCCTTCATCGCCAAACTACTGCCCAAACTCAAAACCGGCGGCTATATCCACCTGGCGACAGACTGGGAAGAATATGCACA
GCAGATGCTTGAAGTCCTCAGTAGCTTCGACAGCCTGCAAAATACGGCGACAGACTACGCCCCTACCCCGGACTACCGCC
CCGAAACCAAATTCGAAGCGCGCGGCAAACGTCTCGGACACGGCGTTTGGGACTTGGTATTCAAACGGATCAGATAA

Upstream 100 bases:

>100_bases
CGAAACGAAACAGGTATAATCGCCCCTTTCCCTTCCCGCCGTCCGAACAGGCCTTTCACATTCAGACGGCATCCTGATTG
CACAAGCGCACGGAAGCATT

Downstream 100 bases:

>100_bases
CAAACCGCTGTTTGAAAAAATGCCGTCTGAAGCATGTTTGCTTACAGACGGCATTTTTTCAAGATAAAGCAGCGAGTGAT
GTTTCGATACAAGGTTTAAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWDSFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATA
EIALRLPETDFLAIDVHGPGVGNLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKFEARGKRLGHGVWDLVFKRIR

Sequences:

>Translated_238_residues
MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWDSFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATA
EIALRLPETDFLAIDVHGPGVGNLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKFEARGKRLGHGVWDLVFKRIR
>Mature_237_residues
TDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWDSFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATAE
IALRLPETDFLAIDVHGPGVGNLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQAP
FIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKFEARGKRLGHGVWDLVFKRIR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=233, Percent_Identity=50.6437768240343, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27076; Mature: 26945

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWDSFGIDYQATPVDLDAN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
FGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVGNLLKLINENHLENIRVMR
CCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
HDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQAPFIAKLLPKLKTGGYIHLAT
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEC
DWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKFEARGKRLGHGVWDLVFKRIR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWDSFGIDYQATPVDLDAN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
FGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVGNLLKLINENHLENIRVMR
CCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
HDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQAPFIAKLLPKLKTGGYIHLAT
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEC
DWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKFEARGKRLGHGVWDLVFKRIR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA