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The map label for this gene is ispG [H]

Identifier: 161870183

GI number: 161870183

Start: 1216034

End: 1217299

Strand: Direct

Name: ispG [H]

Synonym: NMCC_1223

Alternate gene names: 161870183

Gene position: 1216034-1217299 (Clockwise)

Preceding gene: 161870182

Following gene: 161870187

Centisome position: 56.47

GC content: 52.45

Gene sequence:

>1266_bases
ATGAACACACTCCAACGCCGCAAGACGCATCAAGTCCGCATCGATCATATTACCGTCGGTTCAGAAGCACCCGTCGTTAT
CCAATCTATGACCAACACCGACACTGCCGATGCAAAAGCCACCGCATTGCAGATTAAGGAATTGAGCGATGCCGGATCCG
AAATGGTGCGTATTACCGTCAACAGCCCCGAAGCCGCGTCCAAAGTTGCCGAAATCCGCCGCCGCTTGGACGATATGGGC
TATGCCACACCGCTTATCGGCGATTTCCACTTCAACGGCGAACGCCTGTTGGCGGAATTTCCAGAATGCGGCAAAGCATT
GTCCAAATACCGCATCAATCCCGGCAATGTCGGCAAAGGCGTAAAAGGCGATGAAAAATTTGCCTTTATGATTCGGACTG
CTGCTGAAAACGATAAAGCCGTCCGCATCGGCGTAAACTGGGGTTCTTTGGATCAAAGCCTCGCCAAACGTATGATGGAT
GCCAACCTCGCTTCTTCCGCGCCGAAACCGCCCGAAGAAGTGACGAAGGAAGCACTGATTGTCTCCGCTTTGGAATCTGC
CGAAAAAGCCGTTCTATTGGGACTGCCCGAAGACAAAATCATCCTGTCGTGCAAAGTCAGCGCGGTTCAGGATTTGATTC
AGGTTTACCGCGAACTGGGCAGCCGTTGCGCCTATCCGCTGCATTTGGGTTTGACCGAAGCCGGTATGGGCAGCAAAGGC
ATTGTCGCATCAACGGCGGCATTATCCGTCTTGCTTCAAGAAGGAATCGGCGACACCATCCGCATTTCACTGACTCCGGA
ACCTGGCAGCCCGCGTACTCAGGAGGTCGTCGTCGGGCAAGAGATTTTACAGACTATGGGATTGCGTTCGTTTACGCCGA
TGGTTACCGCCTGCCCCGGCTGCGGGCGTACCACCAGTACCGTATTTCAAGAGCTGGCACAAGATGTTCAAAATTACCTG
CGCCAAAAAATGTCTATATGGCGTACCCTTTATCCTGGGGTTGAATCCCTGAACGTTGCCGTAATGGGCTGCGTTGTCAA
TGGGCCCGGAGAAAGCAAATTGGCCGACATCGGCATCAGCCTGCCCGGTACGGGGGAAACACCCGTCGCGCCTGTTTATG
TAGATGGTGAACGCAAAGTAACGCTGAAAGGCGACAACATTGCAACGGAATTTTTGGCTATTGTTGAAGAGTATGTCAAA
ACCAACTACGGCGAAAACGGCTCCAAACGCAACCAGAACAAAATCATCCCAATACAGTCCCTATAA

Upstream 100 bases:

>100_bases
ACAGGCGGCATACGAATATGAAGCACAATTGCAGGCGAATTTCCCCTACTCGGAAGAATTGCAAACCGTCCTCACCGGTC
AATAAACAGATTCAAACCAT

Downstream 100 bases:

>100_bases
GACAAATGCCGTCTGAACTGTTTTCAGACGGCATTTGTTTCTCTCAGATTTCTTATTAAAACTGTAAAGAAGCACGGTTC
TCCAAGATTTGATCAATCAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQIKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMG
YATPLIGDFHFNGERLLAEFPECGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRELGSRCAYPLHLGLTEAGMGSKG
IVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEVVVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYL
RQKMSIWRTLYPGVESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFLAIVEEYVK
TNYGENGSKRNQNKIIPIQSL

Sequences:

>Translated_421_residues
MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQIKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMG
YATPLIGDFHFNGERLLAEFPECGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRELGSRCAYPLHLGLTEAGMGSKG
IVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEVVVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYL
RQKMSIWRTLYPGVESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFLAIVEEYVK
TNYGENGSKRNQNKIIPIQSL
>Mature_421_residues
MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQIKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMG
YATPLIGDFHFNGERLLAEFPECGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRELGSRCAYPLHLGLTEAGMGSKG
IVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEVVVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYL
RQKMSIWRTLYPGVESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFLAIVEEYVK
TNYGENGSKRNQNKIIPIQSL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=390, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45374; Mature: 45374

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQIKELSDAGSEMVRITV
CCCHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEE
NSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPECGKALSKYRINPGNVGKG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCHHHHHHHHHCCCCCCCCCCC
VKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMDANLASSAPKPPEEVTKEALI
CCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
VSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRELGSRCAYPLHLGLTEAGMGSKG
HHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
IVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEVVVGQEILQTMGLRSFTPMVTACPG
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCC
CGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGVESLNVAVMGCVVNGPGESKLADIGIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCCCCCEEECEE
LPGTGETPVAPVYVDGERKVTLKGDNIATEFLAIVEEYVKTNYGENGSKRNQNKIIPIQS
CCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECC
L
C
>Mature Secondary Structure
MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQIKELSDAGSEMVRITV
CCCHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEE
NSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPECGKALSKYRINPGNVGKG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCHHHHHHHHHCCCCCCCCCCC
VKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMDANLASSAPKPPEEVTKEALI
CCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
VSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRELGSRCAYPLHLGLTEAGMGSKG
HHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
IVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEVVVGQEILQTMGLRSFTPMVTACPG
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCC
CGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGVESLNVAVMGCVVNGPGESKLADIGIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCCCCCEEECEE
LPGTGETPVAPVYVDGERKVTLKGDNIATEFLAIVEEYVKTNYGENGSKRNQNKIIPIQS
CCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA