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The map label for this gene is uraA [H]

Identifier: 161870187

GI number: 161870187

Start: 1222245

End: 1223456

Strand: Direct

Name: uraA [H]

Synonym: NMCC_1227

Alternate gene names: 161870187

Gene position: 1222245-1223456 (Clockwise)

Preceding gene: 161870183

Following gene: 161870189

Centisome position: 56.76

GC content: 55.69

Gene sequence:

>1212_bases
ATGAACCAACTGAAACTTGCCGTTTCCGGCGCGCAGATTTTATTTGTGGCATTCGGCGCAATGGTGCTGGTTCCCCTGCT
GACCGGTCTGAATCCGGCTCTTGCGCTTTTGGGCGCAGGCTTGGGAACGCTGCTGTTCCAAATCACAACCAAACGCAAAG
TGCCGATTTTTCTTGGTTCTTCGTTTGCCTTTATCGCACCGATTATCTACTCCGTCGGCGAATGGGGGCTGCCTTCCACC
ATGTTCGGACTGTTTGCCGCCGGCTTTATGTATTTTGTGTTTGCCGCGCTGATCCGTTGGCGCGGACTGGCAGCGGTACA
CAAACTGCTGCCTCCGGTCGTCATCGGCCCCGTCATCATGGTCATCGGTCTGTCTGTTGCCGTGGCGGCAAGCAGCATGG
CAATGGGTCAGGCGGACGGCAAACAGGTCATCGACTATACCGATTCGCTGATTCTTTCCGGCTTTACCTTTGCCGTTACC
GCCATCGTATCGGTTTTCGGCAGCAGGATGATGAAGCTGATTCCCATCTTGATCGGTGTCGCTTCGGGTTATGTTTTGGC
ACTGCTGATGGGACTGGTGGACACGGCAAGCATTGCACACGCGCCCTGGTTCGCCGTTCCCCATTTTGAAACGCCTCAGA
TCAACTGGCAGGCTGCACTGTTTATGCTGCCCGTTGCCGTCGCCCCCGCCATCGAACACATCGGCGGCATCATGGCAATC
GGCAATGTGACGGGGAAAGACTATACGAAAGACCCGGGCTTGGACAAAACCCTTGCAGGCGACGGTTTGGGCGTATGCGT
TGCGGGTCTGATCGGCGGCCCGCCGGTTACGACCTACGGCGAAGTAACGGGTGCGGTGATGATTACCAAAAACAGCAACC
CCGTCATCATGACTTGGGCGGCGGTTTTTGCCGTCTGCATGGCGTTTTTCGGCAAATTCAATGCGTTTTTGGCTTCCATT
CCGATGCCAGTAATGGGCGGCATTATGCTGCTGCTGTTCGGCACGATTGCTTCTTTGGGCGTGAAAACGCTGATTGATGC
CAAAGTCGATTTGATGCTGCCGAAAAACCTGGTCATCGTCAGCTCGGTACTGACCACGGGCATCGGCGGCATGACGCTCA
AATTGGGCAGCTTCAGCTTTGCCGGCGTGGGCTTGTGCGCCGTACTTGCCATTATGTTGAACAGCCTGCTGCCCGATCCG
AAAGAATCCTGA

Upstream 100 bases:

>100_bases
TTTCAGACGGCATATCTGTTGCCCGACGTATGTATTTTTACGCAGACCCTCGGCAAACCAGTATAATCCGTGCCGTTTGA
ACCGATTGAAAGAAGATGGT

Downstream 100 bases:

>100_bases
CCGTCGATATAGAAATGCCGTCTGAACATCTTTCAGACGGCATTTTCCGTTTTATTTGAGATTTTGAATCAAAGAGCGCA
CAGTTCCGCCGTAATAAGAA

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTLLFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPST
MFGLFAAGFMYFVFAALIRWRGLAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQAALFMLPVAVAPAIEHIGGIMAI
GNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPVTTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASI
PMPVMGGIMLLLFGTIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIMLNSLLPDP
KES

Sequences:

>Translated_403_residues
MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTLLFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPST
MFGLFAAGFMYFVFAALIRWRGLAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQAALFMLPVAVAPAIEHIGGIMAI
GNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPVTTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASI
PMPVMGGIMLLLFGTIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIMLNSLLPDP
KES
>Mature_403_residues
MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTLLFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPST
MFGLFAAGFMYFVFAALIRWRGLAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQAALFMLPVAVAPAIEHIGGIMAI
GNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPVTTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASI
PMPVMGGIMLLLFGTIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIMLNSLLPDP
KES

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680143, Length=376, Percent_Identity=23.6702127659574, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI44680148, Length=374, Percent_Identity=23.7967914438503, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316845, Length=374, Percent_Identity=23.7967914438503, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI44680145, Length=372, Percent_Identity=23.6559139784946, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788843, Length=384, Percent_Identity=38.8020833333333, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87081818, Length=410, Percent_Identity=35.609756097561, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87082181, Length=416, Percent_Identity=27.6442307692308, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790087, Length=367, Percent_Identity=25.0681198910082, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082178, Length=178, Percent_Identity=27.5280898876405, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558856, Length=169, Percent_Identity=27.2189349112426, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17542262, Length=231, Percent_Identity=25.974025974026, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21356175, Length=409, Percent_Identity=24.6943765281174, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 41843; Mature: 41843

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTLLFQITTKRKVPIFLGS
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEEECC
SFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRGLAAVHKLLPPVVIGPVIM
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVTAIVSVFGSRMMKLIPILIGV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQAALFMLPVAVAPAIEHIGGIMAI
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPVTTYGEVTGAVMITKNSNPVIMTWA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEHH
AVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFGTIASLGVKTLIDAKVDLMLPKNLVIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCHHHHH
SSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIMLNSLLPDPKES
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTLLFQITTKRKVPIFLGS
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEEECC
SFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRGLAAVHKLLPPVVIGPVIM
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVTAIVSVFGSRMMKLIPILIGV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQAALFMLPVAVAPAIEHIGGIMAI
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPVTTYGEVTGAVMITKNSNPVIMTWA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEHH
AVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFGTIASLGVKTLIDAKVDLMLPKNLVIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCHHHHH
SSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIMLNSLLPDPKES
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]