Click here to switch to the map view.

The map label for this gene is nrdB [H]

Identifier: 161870162

GI number: 161870162

Start: 1191798

End: 1192952

Strand: Reverse

Name: nrdB [H]

Synonym: NMCC_1201

Alternate gene names: 161870162

Gene position: 1192952-1191798 (Counterclockwise)

Preceding gene: 161870163

Following gene: 161870161

Centisome position: 55.4

GC content: 47.71

Gene sequence:

>1155_bases
ATGTCCTGCGAACACTTAACTATGTCATACAGCACCTTTCCCAAAACCAAAAACGACGCGCTGAATGAGCCGATGTTTTT
TGGTCAGCCTGTGAATGTCGCCCGTTATGACCAGCAGAAATACGAGGTATTTGAAAAACTGATTGAAAAACAATTGTCGT
TTTTTTGGCGGCCGGAAGAAATCGACGTGTCGCGCGACCGTATCGACTACGCCAACCTGCCTGAACACGAAAAACATATT
TTCATCAGCAATCTGAAATACCAAACACTGCTCGATTCCATCCAAGGGCGCAGTCCGAATGTTGCCTTGCTGCCTTTGGT
TTCGATTCCCGAGTTGGAAACGTGGATTGAAACGTGGAGCTTCAGCGAAACCATACACTCGCGCAGCTATACCCACATCA
TCCGCAATATTGTGAATGATCCGTCGGTCGTGTTTGATGATATTGTCGAAAACGAATACATTACCGCCCGCGCCGAAGAC
ATTGCCTGCTATTACGATGACTTAATCGAATACACCCAGTATTACAACCTGTTGGGCGAAGGGATGCACAATGTCGGCGG
AAAACCCGTTACCGTATCTTTGCGCGGGTTGAAGAAAAAACTCTATCTCTGCCTGATGTGCGTCAACGTGTTGGAAGCCA
TCCGTTTCTACGTTTCATTCGCCTGCTCGTTTGCTTTTGCCGAGCGCGAGTTGATGGAAGGCAACGCCAAAATCATCAAA
CTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACGCAGCATATGCTTAATCTGATGCGTTCTGGTGTTGATGATTC
TGAAATGGCGGAAATTGCCGCCGAGTTGCAGGACGAATGTTTCCAACTCTTCAAAAAAGCGGCGGAACAGGAAAAAGAAT
GGGCGGCATATTTGTTTAAGGACGGTTCGATGATCGGTTTGAACAAAGAAATCTTATCCCAATACGTCGAATATATTACC
AATCTGCGTATGCAGGCGGTGGGGTTGCCGGCCGGATTTGAAGGCGCAAATCAAAACCCGATTCCGTGGATTAATGCGTG
GCTGTCGTCCGACAACGTACAGGTCGCGCCGCAGGAAGTGGAAATATCCTCTTATTTGATCGGTCAGATAGATTCTGAAG
TGAATACGGATGATTTGGGCGATTTTGAGTTGTAA

Upstream 100 bases:

>100_bases
AACCCATGTTTTAACTTTAGAAAATTTTATTAAATATATAAAGTCTCAGCAGGTTAGTTAAAACTTACAGATCATTTTTA
ATTAATATAGAGAATAGATT

Downstream 100 bases:

>100_bases
ATGTTTTTTGAAAAAATGCCGTCTGAAGCCGGATGTTTCAGACGGCATTTGTATGTTGTATGATGGGAAAAACAGCGGGT
AGGATCGGTTATTGTTCCTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSCEHLTMSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFFWRPEEIDVSRDRIDYANLPEHEKHI
FISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAED
IACYYDDLIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAERELMEGNAKIIK
LIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQLFKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYIT
NLRMQAVGLPAGFEGANQNPIPWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL

Sequences:

>Translated_384_residues
MSCEHLTMSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFFWRPEEIDVSRDRIDYANLPEHEKHI
FISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAED
IACYYDDLIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAERELMEGNAKIIK
LIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQLFKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYIT
NLRMQAVGLPAGFEGANQNPIPWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL
>Mature_383_residues
SCEHLTMSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFFWRPEEIDVSRDRIDYANLPEHEKHIF
ISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDI
ACYYDDLIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAERELMEGNAKIIKL
IARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQLFKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITN
LRMQAVGLPAGFEGANQNPIPWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=377, Percent_Identity=81.9628647214854, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1789031, Length=269, Percent_Identity=24.907063197026, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 44078; Mature: 43947

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCEHLTMSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFFWRPEE
CCCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWS
CCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
FSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDDLIEYTQYYNLLGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
GMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAERELMEGNAKIIK
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEE
LIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQLFKKAAEQEKEWAAYLFK
EEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPIPWINAWLSSDNVQVAPQEV
CCCEECCCHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCHHHHEECCCCCEECCHHH
EISSYLIGQIDSEVNTDDLGDFEL
HHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SCEHLTMSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFFWRPEE
CCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWS
CCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
FSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDDLIEYTQYYNLLGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
GMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAERELMEGNAKIIK
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEE
LIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQLFKKAAEQEKEWAAYLFK
EEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPIPWINAWLSSDNVQVAPQEV
CCCEECCCHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCHHHHEECCCCCEECCHHH
EISSYLIGQIDSEVNTDDLGDFEL
HHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]