Click here to switch to the map view.

The map label for this gene is ecoRIIR [H]

Identifier: 161870163

GI number: 161870163

Start: 1192992

End: 1194179

Strand: Reverse

Name: ecoRIIR [H]

Synonym: NMCC_1202

Alternate gene names: 161870163

Gene position: 1194179-1192992 (Counterclockwise)

Preceding gene: 161870164

Following gene: 161870162

Centisome position: 55.46

GC content: 31.73

Gene sequence:

>1188_bases
ATGAATGACTTGGCAGCTCAGACAATACAAGTAGTAAATAAATCCGATGACTTAGTAGCTTCAGCAATACAGACAGCAAA
TAAATCAATTGCAGTATACTGTCGTTATATTCGTCCCAATGATGTTGGTACTACTGGTAGTCACCAATCAGGATTTTATA
TTCAAAAACATTTTTCAGACGACCTCTTTGATGTAGTTTGCCAAAAGGGAACAAATAAAACTATTTCAATCAAAATTAAT
TGGCAAGATGGAAGCGTCACTAATAGCAATTTTAAATATTACGGCCAAGGCACGAGAAATGAGGCGAGAATTACGGGTTT
TGGCAAGAATTTTGAATTTTTAAGTGATAAATATAGTGGTTCCTTATTGGTATTGTGCAGAGCTTGTTATAAAGATTTAT
TATTCCATGCTTTTGTTTTATCTTCAGATGAAGATATTGAAATATTTATCGCAGAAACTAACATTTTACCAGGAAGTTTA
TATTTACCTAAAAAACAAGAAGTAGAGGATAACCTAACAAAATTATTTTCACTATTTCCTAACTTCCCCAAAACTGAAGA
AATGGCAGTTTTGGCAAGGGAAAATATTGCATTAAATAAAACTAACGTTAGTAATGTGTTAAAACAATGGGTTTCAAAGG
AATATGAATTATTTTCCATTTTTGAAAAACGTGAATTTGAAATTATTAAATCAAAAATTACTGATTTGGATAGCTTCATT
AATTTTGCACATTCTTTTACAAATCGCAGAAAAGCAAGAGCAGGAAAATCTTTAGAGTTACATTTATCAAGAATATTTGA
CGAATTTTCCTTAAAATTTGAGACGCAAGCGAAGACAGAAGGGAAAAAGAAACCAGATTTTTTATTTCCTGGAAGTGAAG
AGTACCATGCGATGGATGAATCAGGGAATTTCATATTTCAAACTGAAAAGCTGACAATGCTAGGTTCAAAGACTACTTGT
AAGGATCGCTGGCGCCAAGTTTTAAATGAAGCAGATCGAATTCCTCATAAGCATTTATTTACTCTACAAGAAGGTATATC
TGATACTCAAATTCAGGAAATGAGTGATGAGAATTTAACCTTAGTTGTCCCTAAAGAATCCGTAAAAACATTTGGAACTT
TTGGGAAAACCCATGTTTTAACTTTAGAAAATTTTATTAAATATATAAAGTCTCAGCAGGTTAGTTAA

Upstream 100 bases:

>100_bases
GATGCAGTATCCAAGACAGCTGCATACAAGCAGTTTGGCAATTCAATTGCTGTACCGTTGGTTCAAGCTATTGCTAAACA
AATTATAAATGAGTTAAAAA

Downstream 100 bases:

>100_bases
AACTTACAGATCATTTTTAATTAATATAGAGAATAGATTATGTCCTGCGAACACTTAACTATGTCATACAGCACCTTTCC
CAAAACCAAAAACGACGCGC

Product: type-II restriction endonuclease

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKIN
WQDGSVTNSNFKYYGQGTRNEARITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELFSIFEKREFEIIKSKITDLDSFI
NFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQAKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTC
KDRWRQVLNEADRIPHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQQVS

Sequences:

>Translated_395_residues
MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKIN
WQDGSVTNSNFKYYGQGTRNEARITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELFSIFEKREFEIIKSKITDLDSFI
NFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQAKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTC
KDRWRQVLNEADRIPHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQQVS
>Mature_395_residues
MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKIN
WQDGSVTNSNFKYYGQGTRNEARITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELFSIFEKREFEIIKSKITDLDSFI
NFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQAKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTC
KDRWRQVLNEADRIPHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQQVS

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 45199; Mature: 45199

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFSD
CCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCEEEECCCC
DLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEARITGFGKNFEFLSDKYSG
HHHHHHHCCCCCCEEEEEEEECCCCEECCCCEEECCCCCCCCEEEECCCCCHHHHCCCCC
SLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSLYLPKKQEVEDNLTKLFSLFP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHCC
NFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELFSIFEKREFEIIKSKITDLDSFI
CCCCCHHHHHHHHCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQAKTEGKKKPDFLFPGSEEYHAMDE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEECCCCCHHEEECC
SGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRIPHKHLFTLQEGISDTQIQEMSDENLT
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCEE
LVVPKESVKTFGTFGKTHVLTLENFIKYIKSQQVS
EEEEHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFSD
CCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCEEEECCCC
DLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEARITGFGKNFEFLSDKYSG
HHHHHHHCCCCCCEEEEEEEECCCCEECCCCEEECCCCCCCCEEEECCCCCHHHHCCCCC
SLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSLYLPKKQEVEDNLTKLFSLFP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHCC
NFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELFSIFEKREFEIIKSKITDLDSFI
CCCCCHHHHHHHHCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQAKTEGKKKPDFLFPGSEEYHAMDE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEECCCCCHHEEECC
SGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRIPHKHLFTLQEGISDTQIQEMSDENLT
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCEE
LVVPKESVKTFGTFGKTHVLTLENFIKYIKSQQVS
EEEEHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]