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The map label for this gene is leuC [H]

Identifier: 161870120

GI number: 161870120

Start: 1149980

End: 1151389

Strand: Direct

Name: leuC [H]

Synonym: NMCC_1159

Alternate gene names: 161870120

Gene position: 1149980-1151389 (Clockwise)

Preceding gene: 161870113

Following gene: 161870121

Centisome position: 53.4

GC content: 56.52

Gene sequence:

>1410_bases
ATGACAGCACAAACCCTCTACGACAAACTTTGGAACAACCACGTCGTCCGCGAAGAAGAAGACGGCACCGTCCTGCTCTA
CATCGACCGCCATTTGGTGCACGAAGTTACCAGCCCTCAGGCATTTGAAGGCTTGAAAATGGCGGGGCGCAAGCTGTGGC
GCATCGACAGCGTCGTCTCCACCGCCGACCACAACACCCCGACCGGCGATTGGGACAAAGGCATCCAAGACCCGATTTCC
AAGCTGCAAGTCGATATTTTGGACAAAAACATTAAAGAGTTTGGCGCACTCGCCTATTTTCCGTTTATGGACAAAGGTCA
GGGCATCGTACACGTTATGGGCCCCGAACAAGGCGCGACCCTGCCCGGTATGACCGTCGTCTGCGGCGACTCGCACACTT
CCACCCACGGCGCATTCGGCGCACTGGCACACGGCATCGGCACTTCCGAAGTCGAGCACACCATGGCGACCCAATGTATT
ACCGCGAAAAAATCCAAATCCATGCTGATTGCCGTTGACGGCAAATTAAAAGCGGGCGTTACCGCCAAAGACGTGGCACT
TTACATCATCGGGCAAATCGGCACGGCGGGCGGCACGGGTTACGCCATCGAGTTTGGCGGCGAAGCCATCCGCAGCCTTT
CTATGGAAGGCCGCATGACTTTATGCAATATGGCGATCGAAGCCGGTGCGCGCTCAGGCATGGTTGCCGTCGACCAAACC
ACCATCGACTATGTAAAAGATAAACCCTTCGCACCTAAAGGCGAAGCATGGGACAAAGCCGTCGAGTACTGGCGCACGCT
GGTGTCTGACGAAGGTGCGGTATTCGACAAAGAATACCGTTTCAACGCCGAAGACATCGAACCGCAAGTCACTTGGGGTA
CCTCGCCTGAAATGGTTTTAGACATCAGCAGCAAAGTGCCGAATCCTGCCGAAGAAACCGATCCGGTCAAACGCAGCGGT
ATGGAACGCGCCCTTGAATACATGGGCTTGGAAGCCGGTACGCCATTAAACGAAATCCCCGTCGACATCGTATTCATCGG
CTCTTGCACCAACAGCCGCATCGAAGACTTGCGCGAAGCCGCCGCCATCGCCAAAGACCGCAAAAAAGCCGCCAACGTAC
AGCGCGTGTTAATCGTCCCCGGCTCCGGTTTGGTTAAAGAACAAGCCGAAAAAGAAGGCTTGGACAAAATTTTCATCGAA
GCCGGTTTTGAATGGCGCGAACCGGGCTGTTCGATGTGTCTCGCCATGAACGCCGACCGCCTGACCCCGGGGCAACGCTG
CGCCTCCACCTCCAACCGCAATTTTGAAGGCCGTCAAGGCAACGGCGGACGTACCCACCTCGTCAGCCCCGCTATGGCAG
CCGCCGCCGCCGTTACCGGCCGCTTTACCGACATCCGCATGATGGCGTAA

Upstream 100 bases:

>100_bases
AACACCGGCTCAAAAATTAGACAAAATTTGTTGCGCGGTATAAAGAATACGTCTAAAATCCGCCCAATCCGCATTACCAT
TTATCCAAAAGAACAACATC

Downstream 100 bases:

>100_bases
GTATTTTCAGGCGGTCTTTAAGAATTTGACGCGGCCGTCTGAAATATGTCCCGTAAGGCAGAAACACCTTTAATATTCAA
ACCAAGGAGTTTAACCATGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTAQTLYDKLWNNHVVREEEDGTVLLYIDRHLVHEVTSPQAFEGLKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPIS
KLQVDILDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQT
TIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSG
MERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIE
AGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAVTGRFTDIRMMA

Sequences:

>Translated_469_residues
MTAQTLYDKLWNNHVVREEEDGTVLLYIDRHLVHEVTSPQAFEGLKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPIS
KLQVDILDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQT
TIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSG
MERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIE
AGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAVTGRFTDIRMMA
>Mature_468_residues
TAQTLYDKLWNNHVVREEEDGTVLLYIDRHLVHEVTSPQAFEGLKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISK
LQVDILDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCIT
AKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQTT
IDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGM
ERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIEA
GFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAVTGRFTDIRMMA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=26.9754768392371, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI41352693, Length=372, Percent_Identity=27.4193548387097, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=64.6680942184154, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=26.0989010989011, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI2367097, Length=369, Percent_Identity=25.4742547425474, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=368, Percent_Identity=30.4347826086957, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=30.4347826086957, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=29.3159609120521, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=417, Percent_Identity=26.378896882494, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=63.9658848614073, Blast_Score=603, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6323335, Length=393, Percent_Identity=27.2264631043257, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=386, Percent_Identity=28.7564766839378, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=419, Percent_Identity=25.0596658711217, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=479, Percent_Identity=26.9311064718163, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=479, Percent_Identity=26.9311064718163, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=27.4809160305344, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=25.9493670886076, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=26.1213720316623, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=26.3852242744063, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50836; Mature: 50705

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTLYDKLWNNHVVREEEDGTVLLYIDRHLVHEVTSPQAFEGLKMAGRKLWRIDSVVS
CCHHHHHHHHHCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TADHNTPTGDWDKGIQDPISKLQVDILDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCITAKKSKSMLIAVDGKLKAGV
CCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQT
CHHHEEEEEEEECCCCCCCEEEEEECHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECC
TIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHCCCEEEECCCCCEEE
DISSKVPNPAEETDPVKRSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREA
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHH
AAIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LTPGQRCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAVTGRFTDIRMMA
CCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TAQTLYDKLWNNHVVREEEDGTVLLYIDRHLVHEVTSPQAFEGLKMAGRKLWRIDSVVS
CHHHHHHHHHCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TADHNTPTGDWDKGIQDPISKLQVDILDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCITAKKSKSMLIAVDGKLKAGV
CCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQT
CHHHEEEEEEEECCCCCCCEEEEEECHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECC
TIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHCCCEEEECCCCCEEE
DISSKVPNPAEETDPVKRSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREA
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHH
AAIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LTPGQRCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAVTGRFTDIRMMA
CCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA