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The map label for this gene is cysG [H]

Identifier: 161870041

GI number: 161870041

Start: 1056531

End: 1057973

Strand: Reverse

Name: cysG [H]

Synonym: NMCC_1074

Alternate gene names: 161870041

Gene position: 1057973-1056531 (Counterclockwise)

Preceding gene: 161870042

Following gene: 161870040

Centisome position: 49.13

GC content: 59.18

Gene sequence:

>1443_bases
GTGAATTATTTCCCCATTTTCGCCAACCTCGCCGGCCGCCCCGTATTGGTCGTAGGCGGCGGTTCGGTCGCCGCGCGCAA
AATCAGCCTGTTGCTGGATGCCGGCGCGCAAGTCAGGGTTGTTGCAAATCAGTTGAATGCTGAATTAAGTGCGCTGGCGG
CAGAAAACAAAATCCTGTGGCTTGCCGAAGAATTTCGTGCCGAACACATCCGCACAGTTTTCCTCATCATTGCGGCAAGC
AGCGACCAAGCCCTCAACCGGCGCGTTTTCCAGCTTGCCGAAAGCTGCCAAAAACCGGTCAACGTGGTGGACGACCGCGA
TTATTGCAGTTTTATTTTTCCTTCGATTATCAATCGCAATCCGATTCAAATTGCCGTTTCCAGTTCGGGCAGCGCGCCCG
TCCTCGCCCGTTTGCTGCGTGAAAAACTGGAAGCCCTGCTGCCTCATTCTTTGGGCGATATGGCGGAAATCTCCGGAAGA
TGGCGCGATGCGGTCAAAGCCAAACTGAAATCCGTTACCGAACGCCGCCGTTTTTGGGAAAAGCAGTTCAACGGCCGTTT
TGCCGCCCTCGTTAAAAATCAACAAACCGCCCAAGCCGAACAGGAGTTGGCAAAGCAGTTGGAACAAAATTATCAAGGCG
GTTTTGTGTCTTTGGTCGGCGCGGGTCCCGGCGATGCGGGGCTGCTGACGCTCAAAGGGCTGCAGGAAATCCAGCAGGCA
GACGTGGTGCTTTACGACGCGCTGGTTTCAGACGGCATACTGTCCCTCGTCCGCCGCGATGCGGAACGGATTTTTGTCGG
CAAGCGCGCCCGGGGCGACCGCACCCCGCAAGAGGACACCAATGCGCTTATGGTCAGGCTGGCCCGCGAAGGCAGGCGCG
TGGTGCGTTTGAAAGGCGGAGACCCTTTCGTGTTCGGACGCGGCGGCGAAGAATTGGAAACCTTGGCACGCCATCAGATT
CCGTTTTCGGTCGTCCCCGGCATTACCGCCGCCGTCGGCGCGACCGCCTATGCCGGTATCCCGCTCACGCACCGCGATTA
TGCCCAAAGTGCGGTTTTCGTAACCGGCCACCGCAAGGCGGATGCGCCCGACATCGAATGGCAGACCCTCGCCCGCAGCC
GCCAGACCTTGGTGATTTATATGGGTGCGCTCAAAGCCGCCCTGATTGCCGAACGGCTGCAACAGCACGGACGCTCGCCC
GACACGCCGGCAGCCGTCATCAGCCAAGGCACGCTGCCGGCTCAAAAAACCGCAACCGGCACGCTCGCCAACCTTGCCGA
ACTCGCCGAAACCGCGCCGAATCCGGCATTGATTGTCATAGGCGAAGTGGTGGGGCTGCACGAAAAACTCGCCTGGTTCG
GTGAAAACGGCGAAGGAGAAAACCGGGTCGGGCAGGCGTATCCCGCATTGGGCGGACTGAATGCCGGACAACGGGCGGCA
TAA

Upstream 100 bases:

>100_bases
GGGGAAATCCTTTTGTCTAAAATGACTCGCGTTCCGACAAAAATACCGCGACGCACCATATCGGAAACAAGGTGCGGGCA
AACAGCAAAGGAGTTCGCCA

Downstream 100 bases:

>100_bases
GGCGGGAAAAATGGAAACGACACTGTTCAAACCCGCCCTGTGGCAGATACCGCATATCGGATCCGGCGGCGAAACCGCCC
TTGCGGAAAAAACGGAAACC

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQLNAELSALAAENKILWLAEEFRAEHIRTVFLIIAAS
SDQALNRRVFQLAESCQKPVNVVDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSLVGAGPGDAGLLTLKGLQEIQQA
DVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQI
PFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAERLQQHGRSP
DTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA

Sequences:

>Translated_480_residues
MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQLNAELSALAAENKILWLAEEFRAEHIRTVFLIIAAS
SDQALNRRVFQLAESCQKPVNVVDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSLVGAGPGDAGLLTLKGLQEIQQA
DVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQI
PFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAERLQQHGRSP
DTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA
>Mature_480_residues
MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQLNAELSALAAENKILWLAEEFRAEHIRTVFLIIAAS
SDQALNRRVFQLAESCQKPVNVVDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSLVGAGPGDAGLLTLKGLQEIQQA
DVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQI
PFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAERLQQHGRSP
DTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=461, Percent_Identity=53.5791757049892, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322922, Length=253, Percent_Identity=35.9683794466403, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51868; Mature: 51868

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQLNAELSALAAENKILW
CCCEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEE
LAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNVVDDRDYCSFIFPSIINRN
EHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
PIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGRWRDAVKAKLKSVTERRRFWE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSLVGAGPGDAGLLTLKGLQEIQQA
HHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHHHHHHH
DVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGG
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECC
DPFVFGRGGEELETLARHQIPFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKA
CEEEECCCCHHHHHHHHHCCCCHHHCCHHHHHCCHHHCCCCCCCHHHHCCEEEEECCCCC
DAPDIEWQTLARSRQTLVIYMGALKAALIAERLQQHGRSPDTPAAVISQGTLPAQKTATG
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHCCH
TLANLAELAETAPNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQLNAELSALAAENKILW
CCCEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEE
LAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNVVDDRDYCSFIFPSIINRN
EHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
PIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGRWRDAVKAKLKSVTERRRFWE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSLVGAGPGDAGLLTLKGLQEIQQA
HHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHHHHHHH
DVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGG
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECC
DPFVFGRGGEELETLARHQIPFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKA
CEEEECCCCHHHHHHHHHCCCCHHHCCHHHHHCCHHHCCCCCCCHHHHCCEEEEECCCCC
DAPDIEWQTLARSRQTLVIYMGALKAALIAERLQQHGRSPDTPAAVISQGTLPAQKTATG
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHCCH
TLANLAELAETAPNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]