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The map label for this gene is cysD [H]

Identifier: 161870039

GI number: 161870039

Start: 1054819

End: 1055742

Strand: Reverse

Name: cysD [H]

Synonym: NMCC_1072

Alternate gene names: 161870039

Gene position: 1055742-1054819 (Counterclockwise)

Preceding gene: 161870040

Following gene: 161870038

Centisome position: 49.03

GC content: 58.77

Gene sequence:

>924_bases
ATGACGAAAACCGAACCGAACAACGCCCAACTCGACTGGTTGGAATCCGAATCCATCCACATCATCCGCGAAGTGGCGGC
GGAATGCGAAAACCCCGCCCTGCTCTTTTCCGGCGGCAAAGATTCCGTGGTCCTGCTCGCCCTCGCCTGCAAAGCCTTCC
GGCTGGGCAGCAGGCCGGTAAAACTGCCCTTCCCGCTGGTGCATATCGACACGGGGCACAACTACCCCGAAGTGATTGCT
TTCCGCGACGCGCAGGCGGCAAAACTCAATGCCCGCCTGATAGTGGGGCGCGTGGAAGACTCCATTGCCAAAGGCACAGT
AGTTTTACGCAAAGAAACCGATTCGCGCAATGCGGCGCAGGCGGTTACGCTGTTGGAAACCATCGAGGCAAACGGCTTTG
ACGCGCTGATGGGCGGCGCGCGGCGCGACGAAGAAAAAGCCCGCGCCAAAGAACGGATTTTCTCGTTCCGAGACGAGTTC
GGACAATGGGATCCGAAGGCGCAACGCCCCGAGCTGTGGTCGCTGTACAACACCAGGCTGCACAAAGGCGAAAATATGCG
CGTCTTCCCGATTTCCAACTGGACGGAACTCGACATCTGGCAATACATCGCCCGCGAAAACCTCGAGCTGCCGCCGATTT
ATTACAGCCACAGGCGCGAAGTGGTCAGACGCAGGGGGCTGCTGGTCCCTGTAACGCCGCTCACCCCGAAAATGCCGTCT
GAAACCTCCGAAATCCTTGATGTCCGCTTCCGCACCGTCGGCGACATCAGCTGCACCTGCCCGGTAGAAAGCACCGCGTC
CACGCCGACGGAGATTATCAGAGAAACAGCGGTTGCCGACATTTCCGAACGCAGCGCGACCCGGCTGGACGATCAGGCAA
GCGAGGCGGCAATGGAAAAACGCAAAAAAGAAGGCTATTTCTAA

Upstream 100 bases:

>100_bases
ATCCGCGCCGGGCGGTGGTGGTGGGAAGGCAGAAACAGCAAAGAATGCGGGCTGCACAAATAATTCATAAATATCAGACA
GAAGAAATAAGGAATATGGT

Downstream 100 bases:

>100_bases
ATTTCAAAAACCCTTTAAGCAGATATGAGGAAGAAAATATGACGGCACAACACCAAACCCCGCTCCGCTTCATTACCGCC
GGCAGCGTCGATGACGGCAA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVVLLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIA
FRDAQAAKLNARLIVGRVEDSIAKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHRREVVRRRGLLVPVTPLTPKMPS
ETSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADISERSATRLDDQASEAAMEKRKKEGYF

Sequences:

>Translated_307_residues
MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVVLLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIA
FRDAQAAKLNARLIVGRVEDSIAKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHRREVVRRRGLLVPVTPLTPKMPS
ETSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADISERSATRLDDQASEAAMEKRKKEGYF
>Mature_306_residues
TKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVVLLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAF
RDAQAAKLNARLIVGRVEDSIAKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEFG
QWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHRREVVRRRGLLVPVTPLTPKMPSE
TSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADISERSATRLDDQASEAAMEKRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=296, Percent_Identity=47.972972972973, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34697; Mature: 34566

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVVLLALACKAFRLGSRPV
CCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCE
KLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSIAKGTVVLRKETDSRNAAQ
ECCCEEEEEECCCCCCCEEEECCCCHHCCCEEEEEEECHHHHHCCEEEEEECCCCCHHHH
AVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNTRL
HHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
HKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHRREVVRRRGLLVPVTPLTPKMPS
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
ETSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADISERSATRLDDQASEAAMEK
CHHHHHHHHHHEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RKKEGYF
HHHCCCC
>Mature Secondary Structure 
TKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVVLLALACKAFRLGSRPV
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCE
KLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSIAKGTVVLRKETDSRNAAQ
ECCCEEEEEECCCCCCCEEEECCCCHHCCCEEEEEEECHHHHHCCEEEEEECCCCCHHHH
AVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNTRL
HHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
HKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHRREVVRRRGLLVPVTPLTPKMPS
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
ETSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADISERSATRLDDQASEAAMEK
CHHHHHHHHHHEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RKKEGYF
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]