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The map label for this gene is cysI

Identifier: 161870036

GI number: 161870036

Start: 1049887

End: 1051656

Strand: Reverse

Name: cysI

Synonym: NMCC_1069

Alternate gene names: 161870036

Gene position: 1051656-1049887 (Counterclockwise)

Preceding gene: 161870037

Following gene: 161870035

Centisome position: 48.84

GC content: 55.25

Gene sequence:

>1770_bases
ATGACCGTACAGACCAAGACAAAAGGTTTGGCGTGGCAAGAAAAACCGCTATCCGACAACGAACGTCTGAAAACCGAAAG
CAATTTTTTACGCGGCACGATTTTGGACGATTTGAAAGACCCGCTCACGGGCGGCTTCAAAGGCGACAACTTCCAACTCA
TCCGCTTCCACGGTATGTATGAGCAGGACGACCGCGACATCCGCGCCGAACGCGCCGAGGCAAAACTCGAGCCCTTGAAA
TTTATGCTTTTGCGCTGCCGGCTGCCGGGCGGGATCATTAAACCGTCCCAATGGATAGAACTGGACAAATTTGCCCGGGA
AAACAGTCATTACCGCTCCATCCGGCTGACCAACCGGCAAACCTTCCAATTTCACGGCGTGCCGAAAGCCAAGTTGCAGA
CGATGCACCGCCTCTTGCACAAACTGGGTTTGGATTCCATCGCCACGGCGGCGGATATGAACCGCAACGTGCTTTGCACG
TCCAACCCGATCGAGTCCGAACTGCACCGGCAGGCTTACGAATACGCGAAAAAGATTTCCGAACACTTGCTGCCGCGCAC
GCGCGGTTATCTGGATGTTTGGGTGGACGGCAAAAAAGTTCAAAGTTCCGACGACTTCCTTCAAGAAGACGAGCCGATTT
TGGGCAAAACCTATCTGCCGCGCAAATTCAAAACCGCAGTCGTCATCCCGCCCTTAAATGATGTGGACTGCTACGGCAAC
GACTTGGATTTTGTCGCCATTTCAGACGGCAACGGACAGCTTGCCGGATTCAATGTTTTGGCGGGCGGCGGGCTTTCGAT
GGAACACGGCAACACCAAAACCTATCCGAACATTTCACTGGAACTGGGTTTCGTGCCTCCGGAACACGCGCTGAAGGCCG
CCGAAGCGGTGGTAACCACACAGCGCGACTTCGGCAACCGCAGCGACCGCAAAAACGCCCGCACCCGCTACACCATTCAA
AATATGGGCTTGGACAACTTCCGCGCCGAAGTCGAACGCCGTATGGGTATGCCGTTCGAACCCATACGCCCGTTCAAATT
TACCGGGCGCGGCGACCGCATCGGCTGGGTAAAAGGCATAGACGGCAACTGGCATTTGACCCTTTTCATCGAAAGCGGCC
GTTTGGTTGACGAAGGCGGGAAACAGCTTCTGACCGGCGTGTTGGAAATCGCCAAAATCCACAAAGGCGATTTCCGCATT
ACCGCCAACCAAAACCTCATCGTGGCAAATGTCGCCGAAGCAGACAAAGCAAAAATCGAAGAACTTGCCCGAACATACGG
ATTAATCCGCAACGATGTCAGCAAACTGCGCGAAAATGCGATGTCGTGCGTTTCCTTCCCGACCTGCCCGCTGGCAATGG
CGGAAGCCGAACGCGTGCTGCCGGACTTCATCGACGAGCTGGATAAGATTATGGCGAAACACGGCACGTCGGACGACTAC
ATCGTTACCCGCATTACCGGCTGCCCCAACGGCTGCGGACGGGCAATGTTGGCGGAAATCGGGCTGGTCGGCAAAGCCGT
CGAACGCTACAACCTCCATATCGGCGGCGACCGTGAAGGCGTACGCATCCCCCGCCTTTATAAAGAAAACATCACCCTGC
CCGAAATCCTTGCCGAATTGGACGACCTGATCGGCAAATGGGCGGCAGAACGCAATATCGGCGAAGGTTTCGGCGATTTC
GCCATACGGACGGGCATCGTCAAACCCGTATTAAATGCACCCGTTGATTTTTGGGACGTATCCAAAGCCGTCGCGATTGC
CCGCGCCTGA

Upstream 100 bases:

>100_bases
TGGACGAAGAGGGCGCAGAAGAATATTTGGATATGCTGCGCGAAGAAAAACGCTATCAGCGTGATGTTTATTGATTAAAT
ATAATCGGGAGGAACACAAA

Downstream 100 bases:

>100_bases
ACCCGTCCCCGCATTCCTGCGGGGATTTTCTTTTTTGCCGCACGCCGTTTCAATCCCGCAAAATTCCGATACTTGGTATA
AAATACCCTCTTTTCCCACT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTGGFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLK
FMLLRCRLPGGIIKPSQWIELDKFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTYLPRKFKTAVVIPPLNDVDCYGN
DLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYPNISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQ
NMGLDNFRAEVERRMGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAKIHKGDFRI
TANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCPLAMAEAERVLPDFIDELDKIMAKHGTSDDY
IVTRITGCPNGCGRAMLAEIGLVGKAVERYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDF
AIRTGIVKPVLNAPVDFWDVSKAVAIARA

Sequences:

>Translated_589_residues
MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTGGFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLK
FMLLRCRLPGGIIKPSQWIELDKFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTYLPRKFKTAVVIPPLNDVDCYGN
DLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYPNISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQ
NMGLDNFRAEVERRMGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAKIHKGDFRI
TANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCPLAMAEAERVLPDFIDELDKIMAKHGTSDDY
IVTRITGCPNGCGRAMLAEIGLVGKAVERYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDF
AIRTGIVKPVLNAPVDFWDVSKAVAIARA
>Mature_588_residues
TVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTGGFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKF
MLLRCRLPGGIIKPSQWIELDKFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCTS
NPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTYLPRKFKTAVVIPPLNDVDCYGND
LDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYPNISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQN
MGLDNFRAEVERRMGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAKIHKGDFRIT
ANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCPLAMAEAERVLPDFIDELDKIMAKHGTSDDYI
VTRITGCPNGCGRAMLAEIGLVGKAVERYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFA
IRTGIVKPVLNAPVDFWDVSKAVAIARA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=566, Percent_Identity=68.904593639576, Blast_Score=831, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=598, Percent_Identity=42.809364548495, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_NEIM0 (A9LZ72)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001599205.1
- ProteinModelPortal:   A9LZ72
- SMR:   A9LZ72
- EnsemblBacteria:   EBNEIT00000011117
- GeneID:   5796005
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_1069
- GeneTree:   EBGT00050000021777
- HOGENOM:   HBG626671
- OMA:   VGDEHIV
- ProtClustDB:   PRK13504
- BioCyc:   NMEN374833:NMCC_1069-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 66120; Mature: 65988

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTGGFKGDNFQLIRFHGMY
CCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
EQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELDKFARENSHYRSIRLTNRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCE
TFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCTSNPIESELHRQAYEYAKKIS
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
EHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTYLPRKFKTAVVIPPLNDVDCYGN
HHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYPNISLELGFVPPEHALKAAEAVVTT
CCCEEEEECCCCCEEEEEEEECCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERRMGMPFEPIRPFKFTGRGDRIGWVKGI
HHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEC
DGNWHLTLFIESGRLVDEGGKQLLTGVLEIAKIHKGDFRITANQNLIVANVAEADKAKIE
CCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHHH
ELARTYGLIRNDVSKLRENAMSCVSFPTCPLAMAEAERVLPDFIDELDKIMAKHGTSDDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IVTRITGCPNGCGRAMLAEIGLVGKAVERYNLHIGGDREGVRIPRLYKENITLPEILAEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHCCCCHHHHHHHH
DDLIGKWAAERNIGEGFGDFAIRTGIVKPVLNAPVDFWDVSKAVAIARA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTGGFKGDNFQLIRFHGMY
CCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
EQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELDKFARENSHYRSIRLTNRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCE
TFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCTSNPIESELHRQAYEYAKKIS
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
EHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTYLPRKFKTAVVIPPLNDVDCYGN
HHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYPNISLELGFVPPEHALKAAEAVVTT
CCCEEEEECCCCCEEEEEEEECCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERRMGMPFEPIRPFKFTGRGDRIGWVKGI
HHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEC
DGNWHLTLFIESGRLVDEGGKQLLTGVLEIAKIHKGDFRITANQNLIVANVAEADKAKIE
CCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHHH
ELARTYGLIRNDVSKLRENAMSCVSFPTCPLAMAEAERVLPDFIDELDKIMAKHGTSDDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IVTRITGCPNGCGRAMLAEIGLVGKAVERYNLHIGGDREGVRIPRLYKENITLPEILAEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHCCCCHHHHHHHH
DDLIGKWAAERNIGEGFGDFAIRTGIVKPVLNAPVDFWDVSKAVAIARA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA