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The map label for this gene is bioB [H]

Identifier: 161870033

GI number: 161870033

Start: 1045245

End: 1046495

Strand: Direct

Name: bioB [H]

Synonym: NMCC_1066

Alternate gene names: 161870033

Gene position: 1045245-1046495 (Clockwise)

Preceding gene: 161870032

Following gene: 161870044

Centisome position: 48.54

GC content: 55.88

Gene sequence:

>1251_bases
ATGCCGTCTGAAAGCCCTTCAGACGGCATCGCCCGGCTGCGCGGCACAAAGGCGGAAAAACCGTTTGCCCCGTATTTTCA
AACGCGTTACACTTGCCGCCGCTGTTTTCAGCCATTTGATTACCCGCAACCGCCGTCATTGCGCCGGCGGTTTGCCTGTC
AGCGTCATTGCGCCGCTGTAAATACGAAAGAACACATTATGACCGTATCCCCCGTCGCCTTGCGCCGTAAGACCGAGTGC
AAGCCTCATCCCACCGCGCGCTATTGGAAAAAATGCGATGTCGAGGCACTTTTCGGACTGCCGTTTTTAGAACTTGTCCA
TCAGGCGGCAGAAGTCCACCGCCAAAATTTCAACCCGCGCGAAATCCAGCTTTCCACGCTTTTGTCCATCAAAACCGGCG
GTTGTCCCGAAGATTGCGCCTACTGTCCGCAATCGGCGCACCACAATACCAATCTGGGCAAAGAGCAGATGATGGATGTG
GATGAAATCGTCGAAAAAGCCAAAATCGCCAAATCGCGCGGCGCAAGCCGGTTTTGTATGGGCGCGGCATGGCGCGGCCC
CAAACCCAAAGACGTGGAGACGGTTTCCGCAATCATCAAAGCCGTCAAGGGCTTGGGTATGGAAACCTGCGGCACGTTCG
GTATGCTCGAAGAAGGTATGGCGGAAGACTTGAAAGAGGCGGGCTTGGATTATTACAACCACAACCTCGACACCGACCCC
GACCGCTACAACGACATCATCCATACACGCCGGCATGAAGACCGTATGGACACCTTGGGCAAAGTCCGCAACGCCGGTTT
GAAAGTCTGCTGCGGCGGCATCGTCGGGATGAACGAAACTCGTGCCGAACGCGCCGGGCTGATTGCCAGCCTCGCCAACC
TCGACCCGCAGCCCGAAAGCGTGCCGATTAACCGGTTGGTCAAAGTGGAAGGCACGCCGCTTGACGATGCCGAAGATTTG
GACTGGACGGAATTTGTCCGCACCATCGCCGTGGCGCGGATTACGATGCCGCAAAGTTATGTCCGGCTGTCGGCAGGGCG
CAGCAATATGCCGGAAGCGATGCAGGCGATGTGCTTTATGGCGGGCGCGAATTCGATTTTTTACGGCGACAAGCTGTTGA
CGACAGGCAATCCTGATGAGGACGGCGACAGAATCCTGATGGAAAAGCTTAACCTGTATCCCTTACAGTTTGAGCTGGAA
GGCGAGGTCGCCGAAGTGGAAAAAGCCTCTGGGATTAAAGTGGATTATTGA

Upstream 100 bases:

>100_bases
TGAAAAACGCCCGAACCGGCGACCATATTTTGGTGATGAGCAACGGCGGTTTCGGCGGAATACACACCAAACTGCTGGAC
GCTTTGAGATAGCCCGGGCG

Downstream 100 bases:

>100_bases
CGATTGCAAAAATGCCGTCTGAAACCCGGAAAAAGGCTTTCAGACGGCATTTGTTCGGACGGTATTTCCAATATCTTTTT
ACCGGCGCGTGATGTTGCCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPSESPSDGIARLRGTKAEKPFAPYFQTRYTCRRCFQPFDYPQPPSLRRRFACQRHCAAVNTKEHIMTVSPVALRRKTEC
KPHPTARYWKKCDVEALFGLPFLELVHQAAEVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDV
DEIVEKAKIAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDP
DRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAERAGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDL
DWTEFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
GEVAEVEKASGIKVDY

Sequences:

>Translated_416_residues
MPSESPSDGIARLRGTKAEKPFAPYFQTRYTCRRCFQPFDYPQPPSLRRRFACQRHCAAVNTKEHIMTVSPVALRRKTEC
KPHPTARYWKKCDVEALFGLPFLELVHQAAEVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDV
DEIVEKAKIAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDP
DRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAERAGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDL
DWTEFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
GEVAEVEKASGIKVDY
>Mature_415_residues
PSESPSDGIARLRGTKAEKPFAPYFQTRYTCRRCFQPFDYPQPPSLRRRFACQRHCAAVNTKEHIMTVSPVALRRKTECK
PHPTARYWKKCDVEALFGLPFLELVHQAAEVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVD
EIVEKAKIAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDPD
RYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAERAGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLD
WTEFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELEG
EVAEVEKASGIKVDY

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=59.0909090909091, Blast_Score=387, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=43.6708860759494, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46542; Mature: 46410

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSESPSDGIARLRGTKAEKPFAPYFQTRYTCRRCFQPFDYPQPPSLRRRFACQRHCAAV
CCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
NTKEHIMTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAAEVHRQNFNPR
CCHHHEEEECHHHHHHCCCCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCH
EIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAKIAKSRGASRFCM
HEEHHHHEEECCCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHH
GAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDP
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCC
DRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAERAGLIASLANLDPQPES
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
VPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFM
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHH
AGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELEGEVAEVEKASGIKVDY
HCCCCEEECCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSESPSDGIARLRGTKAEKPFAPYFQTRYTCRRCFQPFDYPQPPSLRRRFACQRHCAAV
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
NTKEHIMTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAAEVHRQNFNPR
CCHHHEEEECHHHHHHCCCCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCH
EIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAKIAKSRGASRFCM
HEEHHHHEEECCCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHH
GAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDP
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCC
DRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAERAGLIASLANLDPQPES
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
VPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFM
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHH
AGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELEGEVAEVEKASGIKVDY
HCCCCEEECCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA