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The map label for this gene is mpl [H]

Identifier: 161870032

GI number: 161870032

Start: 1043860

End: 1045236

Strand: Direct

Name: mpl [H]

Synonym: NMCC_1065

Alternate gene names: 161870032

Gene position: 1043860-1045236 (Clockwise)

Preceding gene: 161870031

Following gene: 161870033

Centisome position: 48.47

GC content: 58.17

Gene sequence:

>1377_bases
ATGAAACACATCCACATTATCGGTATCGGCGGCACGTTTATGGGTGGGATTGCCGCCATTGCCAAAGAAGCAGGGTTTGA
AGTCAGCGGTTGCGATGCGAAGATGTATCCGCCGATGAGCACCCAGCTCGAAGCCTTGGGCATAGGCGTACACGAAGGCT
TCGACGCGGCGCAGTTGGAGGAATTTAAAGCCGACGTTTACGTCATCGGCAATGTCGCCAAGCGCGGGATGGATGTGGTT
GAGGCGATTTTGAACCGCGGGCTGCCTTATATTTCCGGTCCGCAATGGCTGGCTGAAAACGTGCTGCACCATCATTGGGT
ACTCGGTGTGGCGGGGACGCACGGTAAAACGACCACCGCGTCCATGCTCGCATGGGTTTTGGAATATGCCGGACTCGCAC
CGGGCTTCCTTATCGGCGGCGTACCGGAAAACTTCAGCGTTTCCGCCCGCCTGCCGCAAACGCCGCGCCAAGACCCGAAC
AGCCAATCGCCGTTTTTCGTCATTGAAGCCGACGAATACGACACCGCGTTTTTCGACAAACGCTCCAAATTCGTGCATTA
CCGTCCGCGTACCGCCGTGTTGAACAATCTGGAATTCGACCACGCCGACATCTTCGCCGATTTGGGCGCGATACAGACCC
AGTTCCACCACCTCGTGCGTACCGTGCCGTCTGAAGGCCTCATCGTCTGCAACGGACGGCAGCAAAGCCTGCAAGACACT
TTGGACAAAGGCTGCTGGACGCCGGTGGAAAAATTCGGCACGGAACACGGCTGGCAGGCCGGCGAAGCCAATGCCGATGG
CTCGTTCGACGTGTTGCTTGACGGCAAAAAAGCCGGACACGTCGCTTGGAGTTTGATGGGCGGACACAACCGCATGAACG
CGCTCGCCGTCATCGCCGCCGCGCGTCATGCCGGAGTCGACATTCAGACGGCCTGCGAAGCCTTGAGCACGTTTAAAAAC
GTCAAACGCCGCATGGAAATCAAAGGCACGGTAAACGGCATCACTGTTTACGACGACTTCGCCCACCATCCGACCGCCAT
CGAAACCACCATAGAAGGTCTGCGTCAGCGCGTCGGAAACGCCCGCATCCTCGCCGTCCTCGAGCCGCGTTCCAACACGA
TGAAGCTGGGCACGATGAAGTCCGCCCTGCCCGAAAGCCTCAAAGAAGCCGACCAAGTGTTCTGCTACGCCGGCGGTGTG
GACTGGAACGTCGCCGAAGCCCTCGCACCTTTGGGCGGCAGGCTGCACGTCGGCAAGGATTTCGATGCCTTCGTTGCCGA
AATTGTGAAAAACGCCCGAACCGGCGACCATATTTTGGTGATGAGCAACGGCGGTTTCGGCGGAATACACACCAAACTGC
TGGACGCTTTGAGATAG

Upstream 100 bases:

>100_bases
CATAAGGATGCTTCGAATACCGGTGCAAAGACGCATTGATTGAAACCGAATCAGCCATGACGGCGTTGAATAATAGAATT
TGAACGATTAGGGAACTCTG

Downstream 100 bases:

>100_bases
CCCGGGCGATGCCGTCTGAAAGCCCTTCAGACGGCATCGCCCGGCTGCGCGGCACAAAGGCGGAAAAACCGTTTGCCCCG
TATTTTCAAACGCGTTACAC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso- diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVV
EAILNRGLPYISGPQWLAENVLHHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHLVRTVPSEGLIVCNGRQQSLQDT
LDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGKKAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKN
VKRRMEIKGTVNGITVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQVFCYAGGV
DWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIHTKLLDALR

Sequences:

>Translated_458_residues
MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVV
EAILNRGLPYISGPQWLAENVLHHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHLVRTVPSEGLIVCNGRQQSLQDT
LDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGKKAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKN
VKRRMEIKGTVNGITVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQVFCYAGGV
DWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIHTKLLDALR
>Mature_458_residues
MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVV
EAILNRGLPYISGPQWLAENVLHHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHLVRTVPSEGLIVCNGRQQSLQDT
LDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGKKAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKN
VKRRMEIKGTVNGITVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQVFCYAGGV
DWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIHTKLLDALR

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=457, Percent_Identity=57.1115973741794, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1786279, Length=388, Percent_Identity=28.8659793814433, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49588; Mature: 49588

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIGVHEGFDAAQLE
CCEEEEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHHH
EFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVLHHHWVLGVAGTHGKTTTA
HHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHH
SMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPNSQSPFFVIEADEYDTAFFDK
HHHHHHHHHHCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHC
RSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHLVRTVPSEGLIVCNGRQQSLQDT
CCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
LDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGKKAGHVAWSLMGGHNRMNALAVIAA
HHCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
ARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGITVYDDFAHHPTAIETTIEGLRQRVGN
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
ARILAVLEPRSNTMKLGTMKSALPESLKEADQVFCYAGGVDWNVAEALAPLGGRLHVGKD
EEEEEEECCCCCCEEECHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCEEECCCC
FDAFVAEIVKNARTGDHILVMSNGGFGGIHTKLLDALR
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC
>Mature Secondary Structure
MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIGVHEGFDAAQLE
CCEEEEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHHH
EFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVLHHHWVLGVAGTHGKTTTA
HHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHH
SMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPNSQSPFFVIEADEYDTAFFDK
HHHHHHHHHHCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHC
RSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHLVRTVPSEGLIVCNGRQQSLQDT
CCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
LDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGKKAGHVAWSLMGGHNRMNALAVIAA
HHCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
ARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGITVYDDFAHHPTAIETTIEGLRQRVGN
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
ARILAVLEPRSNTMKLGTMKSALPESLKEADQVFCYAGGVDWNVAEALAPLGGRLHVGKD
EEEEEEECCCCCCEEECHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCCCCEEECCCC
FDAFVAEIVKNARTGDHILVMSNGGFGGIHTKLLDALR
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]