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The map label for this gene is dnaN [H]

Identifier: 161869313

GI number: 161869313

Start: 323014

End: 324117

Strand: Direct

Name: dnaN [H]

Synonym: NMCC_0318

Alternate gene names: 161869313

Gene position: 323014-324117 (Clockwise)

Preceding gene: 161869312

Following gene: 161869318

Centisome position: 15.0

GC content: 56.16

Gene sequence:

>1104_bases
ATGTTGATTTTACAAGCCGAACGCGACAGCCTGCTCAAGCCGCTGCAAGCCGTTACCGGCATCGTCGAACGCCGCCACAC
CCTGCCCATCCTGTCCAACGTCCTGATTGAGGGCAAAGGCGGTCAGACCAAACTCTTGGCAACCGATTTGGAAATCCAAA
TCGACACTGCGGGTCCCGAGGGCGGCGCGGACGACTTCCGCATCACCACCAATGCCAAGAAATTTCAGGACATTTTGCGC
GCGCTGCCTGCCGGTGCGCTGGTGTCGCTGGATTGGGACGACAGCCGTCTGACGCTGAAGGCGGGCAAATCGCGTTTTGC
CCTGCAGACCCTGCCTGCCGCCGATTTTCCGATGATGAATGTCGGCGAGGACATCAGCGCGACTTTCTCGCTGGAGCAGG
AGCGTTTCAAAACCATGCTGTCGCAAGTGCAGTACAGCATGGCGGTGCAGGACATCCGCTATTATCTCAACGGTCTGCTG
ATGCAGGTTGAGGGTAGCCAGTTGCGCCTTGTGGCAACCGATGGACACCGCCTTGCCTATGCAGCCTGCGCGATTGATGC
GGATTTGCCGCGCGCCGAAGTGATTTTGCCGCGCAAAACGGTGCTGGAACTGTTCAAACTGTTGAACAACCCCGACGATC
CGATTCAAATCGAGCTGCTGGACAAGCAGGTGCGTTTCCAATGCAACGGCACGACCATCGTCAGCAAGGTCATCGACGGC
AAATTCCCCGATTTCAACCGCGTCATCCCTTTGGACAACGACAAGATTTTCGTTTTGTCCCGTGCCGAACTTTTGGGCGC
GCTGGAACGTGTGTCCATTCTTGCCAACGAAAAATTCCGTGGCGCGCGCCTGTTCCTGCAACCCGGCCTGTTGAGCGTTG
TGTGCAGCAACAACGAGCAGGAAGAAGCGCGCGAAGAAATCGAGATTGCCTATCAGGGCGGCGAACTCGAAGTCGGTTTC
AATATCGGCTATCTGATGGACGTGTTGCGCAACATCCATTCCGACGATATGCAGCTTGCCTTCGGCGATGCCAACCGCTC
GACGCTGTTTACCGTGCCGAACAATCCGAATTTCAAATATATTGTGATGCCGATGCGGATTTGA

Upstream 100 bases:

>100_bases
CAAGTGAATCGGTTCCGTACTATTTGTACTGTCTGCGGCTTCGTCGCCTTGTCCTGATTTAAATTTAATCCACTATACAA
ACCGATTTAAAGGAGCGAAC

Downstream 100 bases:

>100_bases
CGGTTTTCCGGAACACGATGCCCGTATTGGAGATATACCCCGAACCGTGCAGACGGATTCGGGGTTTTGTTCGGCTGTCG
GAAAGGTATAGTGGATTAAC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTKLLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILR
ALPAGALVSLDWDDSRLTLKAGKSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIELLDKQVRFQCNGTTIVSKVIDG
KFPDFNRVIPLDNDKIFVLSRAELLGALERVSILANEKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGF
NIGYLMDVLRNIHSDDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI

Sequences:

>Translated_367_residues
MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTKLLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILR
ALPAGALVSLDWDDSRLTLKAGKSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIELLDKQVRFQCNGTTIVSKVIDG
KFPDFNRVIPLDNDKIFVLSRAELLGALERVSILANEKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGF
NIGYLMDVLRNIHSDDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI
>Mature_367_residues
MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTKLLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILR
ALPAGALVSLDWDDSRLTLKAGKSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIELLDKQVRFQCNGTTIVSKVIDG
KFPDFNRVIPLDNDKIFVLSRAELLGALERVSILANEKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGF
NIGYLMDVLRNIHSDDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=40.9836065573771, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40940; Mature: 40940

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTKLLATDLEIQIDTAGPE
CEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCCEEEEEEEEEEEEECCCCC
GGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAGKSRFALQTLPAADFPMMN
CCCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEECCCCCCCCCC
VGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLLMQVEGSQLRLVATDGHRLAY
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCEEEE
AACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIELLDKQVRFQCNGTTIVSKVIDG
EEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEEHHHHCC
KFPDFNRVIPLDNDKIFVLSRAELLGALERVSILANEKFRGARLFLQPGLLSVVCSNNEQ
CCCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCH
EEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHSDDMQLAFGDANRSTLFTVPNNPNFKY
HHHHHHEEEEEECCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCEEE
IVMPMRI
EEEEECC
>Mature Secondary Structure
MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTKLLATDLEIQIDTAGPE
CEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCCEEEEEEEEEEEEECCCCC
GGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAGKSRFALQTLPAADFPMMN
CCCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEECCCCCCCCCC
VGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLLMQVEGSQLRLVATDGHRLAY
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCEEEE
AACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIELLDKQVRFQCNGTTIVSKVIDG
EEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEEHHHHCC
KFPDFNRVIPLDNDKIFVLSRAELLGALERVSILANEKFRGARLFLQPGLLSVVCSNNEQ
CCCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCH
EEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHSDDMQLAFGDANRSTLFTVPNNPNFKY
HHHHHHEEEEEECCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCEEE
IVMPMRI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]