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The map label for this gene is otsA [H]

Identifier: 161525317

GI number: 161525317

Start: 2364929

End: 2366305

Strand: Reverse

Name: otsA [H]

Synonym: Bmul_2147

Alternate gene names: 161525317

Gene position: 2366305-2364929 (Counterclockwise)

Preceding gene: 161525318

Following gene: 161525315

Centisome position: 68.62

GC content: 67.54

Gene sequence:

>1377_bases
ATGAGTCGACTGATCATTGTTTCGAACCGCGTCGCCCCGATCTCGGAAGGCGAACCGGCGGCGGGCGGGCTCGCGATCGG
CGTCTACGATGCGCTGAAGGAGACGGGCGGCATGTGGTTCGGCTGGAGCGGCGAAGTCGTCGCGTCCGGGGCGCCGCAGA
TCCGCGTCGAGGAGCGCGGGCCCGTGACGTTCGCGACGGTCGGCCTGTCGCGCCGCGATTACGACCAGTACTACCGCGGC
TTCTCGAACGCGACGCTGTGGCCCGCGTTTCATTACCGCGCGGACCTGATCCAGTACGATCGGCACGAATTCGACGGCTA
TCGGCGCGTGAACGTCTGGCTCGCGCAGCAGCTCGTGCCGCTGCTGCAGGACGACGACGTGATCTGGGTGCACGACTATC
ACCTGATTCCGTTCGCGCGCGCGCTGCGCGCGGCCGGCGTGAAGAACCGCATCGGCTTTTTCCTGCATATCCCGTTTCCG
GCCGCGCAGGTGCTCGTCAACGTGCCGCCGCATCGCGAGCTCGTCGAGTCGTTGTGCGCGTTCGACCTGCTCGGCTTCCA
GACCGAACCCGATCTGCGCGCGTTCTGCGACTACATCGAATTCGAAGGCGGCGGCGAGATCGCGCGCGACGGCCGCACGG
TGCGCGTGCAGGCGTTCGGCCAGACGCTGCATGCGGCCGCCTATCCGATCGGGGTGTATCCGGACGAGATCGCGTCGCTC
GCGCAGGCCGGCGAGAACGGCAAGGCGGTGCGCACGCTCGCGACGTCGCTGCGCGGGCGGCAACTGATCATGAGCGTCGA
TCGTCTCGATTATTCGAAGGGGCTCGTCGAGCGCTTCCGTGCGTTCGAGAAACTGCTCGAGCATCAGGCCTCGTTCCGCA
ATCGCGTGTCGTTCCTGCAGATCGCGCCGTCGACACGCGCGGACCTGCGCGCCTATCAGGACATCCGCCTGCAGCTCGAG
GCGGAGTCGGGCCGCATCAACGGCCGCTATGCGGAACTCGACTGGGCGCCGATTCTCTACATCCACCGCCAGTACGAGCG
GCAGGTGCTGGCCGCGTTGTATCGGCTCGCGCGCGTCGGCTTCGTCACGCCGCTGCGCGACGGGATGAACCTCGTGGCGA
AGGAATACGTGTCCGCGCAGAACCCGGACGATCCCGGCGTGCTCGTGCTGTCGCGCTTCGCCGGTGCCGCACGCGAGCTG
ACCGGCGCGCTGATCGTCAATCCGATCGACATCGAGGGCATGGCCGACGCGCTGTCGCAGGCGCTGACGATGCCGCTCGC
AGAGCGCCGCGCGCGCTACGCGGACATGATCGCGCAGCTGCGCGAGAACAACGTGTCGGTGTGGCGCGACCATTTCCTGC
GCGATCTGCAGGGGTAG

Upstream 100 bases:

>100_bases
AGATCGGCGCGGGCGAAACGTCGGCGCGCATGCGGCTCGATTCGGTCGACGCGCTGCATGCGCAGATCGCGCGCTGGCTC
GGTGCGGGGCAGCCGGGCGC

Downstream 100 bases:

>100_bases
CGGGCGCGGAGGATCCGGCGTCGGACGGTGCCGCGCGTCGGGATCGATTGCGCGGCACCGTCGCCACTGCTTGCATGCAC
CGCGTACGCAGAGCGCGCGA

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEERGPVTFATVGLSRRDYDQYYRG
FSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYIEFEGGGEIARDGRTVRVQAFGQTLHAAAYPIGVYPDEIASL
AQAGENGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASFRNRVSFLQIAPSTRADLRAYQDIRLQLE
AESGRINGRYAELDWAPILYIHRQYERQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAAREL
TGALIVNPIDIEGMADALSQALTMPLAERRARYADMIAQLRENNVSVWRDHFLRDLQG

Sequences:

>Translated_458_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEERGPVTFATVGLSRRDYDQYYRG
FSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYIEFEGGGEIARDGRTVRVQAFGQTLHAAAYPIGVYPDEIASL
AQAGENGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASFRNRVSFLQIAPSTRADLRAYQDIRLQLE
AESGRINGRYAELDWAPILYIHRQYERQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAAREL
TGALIVNPIDIEGMADALSQALTMPLAERRARYADMIAQLRENNVSVWRDHFLRDLQG
>Mature_457_residues
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEERGPVTFATVGLSRRDYDQYYRGF
SNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPA
AQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYIEFEGGGEIARDGRTVRVQAFGQTLHAAAYPIGVYPDEIASLA
QAGENGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASFRNRVSFLQIAPSTRADLRAYQDIRLQLEA
ESGRINGRYAELDWAPILYIHRQYERQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARELT
GALIVNPIDIEGMADALSQALTMPLAERRARYADMIAQLRENNVSVWRDHFLRDLQG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=459, Percent_Identity=52.9411764705882, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI115533558, Length=425, Percent_Identity=29.1764705882353, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI115533560, Length=425, Percent_Identity=29.1764705882353, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71987012, Length=428, Percent_Identity=28.5046728971963, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=30.6382978723404, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323537, Length=444, Percent_Identity=25.9009009009009, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320279, Length=424, Percent_Identity=25.4716981132075, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6323917, Length=377, Percent_Identity=25.7294429708223, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.9794238683128, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 51478; Mature: 51347

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEERG
CCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCEEEEECCC
PVTFATVGLSRRDYDQYYRGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
LLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVESLCA
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHH
FDLLGFQTEPDLRAFCDYIEFEGGGEIARDGRTVRVQAFGQTLHAAAYPIGVYPDEIASL
HHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHH
AQAGENGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASFRNRVSFLQ
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAPSTRADLRAYQDIRLQLEAESGRINGRYAELDWAPILYIHRQYERQVLAALYRLARVG
ECCCCHHHHHHHEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
FVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARELTGALIVNPIDIEGMADALSQ
CCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHHCCEEEECCCCCCHHHHHHHH
ALTMPLAERRARYADMIAQLRENNVSVWRDHFLRDLQG
HHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEERG
CCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCEEEEECCC
PVTFATVGLSRRDYDQYYRGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
LLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVESLCA
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHH
FDLLGFQTEPDLRAFCDYIEFEGGGEIARDGRTVRVQAFGQTLHAAAYPIGVYPDEIASL
HHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHH
AQAGENGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASFRNRVSFLQ
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAPSTRADLRAYQDIRLQLEAESGRINGRYAELDWAPILYIHRQYERQVLAALYRLARVG
ECCCCHHHHHHHEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
FVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARELTGALIVNPIDIEGMADALSQ
CCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHHCCEEEECCCCCCHHHHHHHH
ALTMPLAERRARYADMIAQLRENNVSVWRDHFLRDLQG
HHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA