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The map label for this gene is otsB [H]

Identifier: 161525318

GI number: 161525318

Start: 2366302

End: 2367054

Strand: Reverse

Name: otsB [H]

Synonym: Bmul_2148

Alternate gene names: 161525318

Gene position: 2367054-2366302 (Counterclockwise)

Preceding gene: 161525322

Following gene: 161525317

Centisome position: 68.64

GC content: 70.52

Gene sequence:

>753_bases
ATGCAATCCGTTCCGGCCTCCCTGTCTCTGACCGATACCGCGTTCTTCTTCGACTTCGACGGCACGCTCGTCGAGCTCGC
CCCGACACCCGACAGCATTCACGTGCCGCGTGCGCTGCCGACGCTGCTCGACGAGCTGCGACGCCGCTCGCACGGCGCGG
TCGCGATCGTGTCGGGCCGCGGCATCGACAGCATCGATGCGTTTCTGAACATGTCCGACCTGCCGGTCGCCGGCCTGCAT
GGCGCCGAGCGGCGCGACGCGAACGGCGATACGCAGCGCATCGGCTTCAACGACGCGCGGCTGCTGCGCATCGAGCGCGA
GCTCGCGGCCGTCGTCGAGCGCCATCCCGGCATGCTGCTCGAGATCAAGGGCGCGGCCGTCGCGCTGCACTACCGCAATG
CGCCCGAGCGTGAGCCGGCGGCGCGCGAGGCAGCCGAGCGGCTCGTCGCCGAATATCCGGACGCCTACGTGCTGCAGCCC
GGCAAGATGGTGTTCGAGATCAAGCCGAAGGGCGTCGACAAGGGGCGCGCGCTGGCGGCGTTCCTCGACGAGCCGCCGTT
CGCGGGCCGCGTTCCGCTGTTCGCCGGCGACGACCTGACCGACGAGAAGGGCTTTGCGGTCGTCAATGCGCGCGGCGGGC
TGTCGATCAAGATCGGCGCGGGCGAAACGTCGGCGCGCATGCGGCTCGATTCGGTCGACGCGCTGCATGCGCAGATCGCG
CGCTGGCTCGGTGCGGGGCAGCCGGGCGCATGA

Upstream 100 bases:

>100_bases
ACGCCGGTTTCGCCCATCTCCGCGCGCCGACGCGGTAAGATGCGCACAATTCCATTCGCGCAGCGGCGGCCCGGCGGGCC
GTCCCTCAGACATCGAATCG

Downstream 100 bases:

>100_bases
GTCGACTGATCATTGTTTCGAACCGCGTCGCCCCGATCTCGGAAGGCGAACCGGCGGCGGGCGGGCTCGCGATCGGCGTC
TACGATGCGCTGAAGGAGAC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPRALPTLLDELRRRSHGAVAIVSGRGIDSIDAFLNMSDLPVAGLH
GAERRDANGDTQRIGFNDARLLRIERELAAVVERHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYPDAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKIGAGETSARMRLDSVDALHAQIA
RWLGAGQPGA

Sequences:

>Translated_250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPRALPTLLDELRRRSHGAVAIVSGRGIDSIDAFLNMSDLPVAGLH
GAERRDANGDTQRIGFNDARLLRIERELAAVVERHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYPDAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKIGAGETSARMRLDSVDALHAQIA
RWLGAGQPGA
>Mature_250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPRALPTLLDELRRRSHGAVAIVSGRGIDSIDAFLNMSDLPVAGLH
GAERRDANGDTQRIGFNDARLLRIERELAAVVERHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYPDAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKIGAGETSARMRLDSVDALHAQIA
RWLGAGQPGA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=219, Percent_Identity=50.2283105022831, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320279, Length=225, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221473368, Length=251, Percent_Identity=27.0916334661355, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20129307, Length=251, Percent_Identity=27.0916334661355, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582490, Length=251, Percent_Identity=27.0916334661355, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19920676, Length=244, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26837; Mature: 26837

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPRALPTLLDELRRRSHGAVAIVSGR
CCCCCCCEEEECEEEEEECCCEEEEEECCCCCEECCHHHHHHHHHHHHCCCCCEEEEECC
GIDSIDAFLNMSDLPVAGLHGAERRDANGDTQRIGFNDARLLRIERELAAVVERHPGMLL
CCCHHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHHHHCCCEEE
EIKGAAVALHYRNAPEREPAAREAAERLVAEYPDAYVLQPGKMVFEIKPKGVDKGRALAA
EECCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCEEEE
FLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKIGAGETSARMRLDSVDALHAQIA
ECCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHH
RWLGAGQPGA
HHHCCCCCCC
>Mature Secondary Structure
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPRALPTLLDELRRRSHGAVAIVSGR
CCCCCCCEEEECEEEEEECCCEEEEEECCCCCEECCHHHHHHHHHHHHCCCCCEEEEECC
GIDSIDAFLNMSDLPVAGLHGAERRDANGDTQRIGFNDARLLRIERELAAVVERHPGMLL
CCCHHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHHHHCCCEEE
EIKGAAVALHYRNAPEREPAAREAAERLVAEYPDAYVLQPGKMVFEIKPKGVDKGRALAA
EECCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCEEEE
FLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKIGAGETSARMRLDSVDALHAQIA
ECCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHH
RWLGAGQPGA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]