Click here to switch to the map view.

The map label for this gene is lutR [H]

Identifier: 161525311

GI number: 161525311

Start: 2356966

End: 2357727

Strand: Reverse

Name: lutR [H]

Synonym: Bmul_2141

Alternate gene names: 161525311

Gene position: 2357727-2356966 (Counterclockwise)

Preceding gene: 161525313

Following gene: 161525307

Centisome position: 68.37

GC content: 70.6

Gene sequence:

>762_bases
ATGTCCGTGCCTACGCTTCCCGCAGCGCCGCGCCGTCGCGCGCGCAGCCTTGCACAAGATGTCGTCGACGCGCTGACCGC
GCAGATCGAAAACGGCACGCTGCGCCCTGGCGACAAGCTGCCGACCGAAACCGAAGTGATGGCGGCGCAGGGGGTGAGCC
GCACGGTCGTGCGCGAGGCGATCTCGCGGATGCAGGCGAGCGGCCTGATCGAGACGCGCCACGGGATCGGCAGCTTCGTG
CTCGAACCGTCGCGCCGCCAGGCACTCGGCATCGACCCCGCGACGATCACGACGCTGCGCGACGTGCTGGCGGTGCTCGA
GCTGCGCATCAGCCTCGAGAGCGAGTGTGCGAGCCTCGCCGCGCAGCGCGCGACGGATGCCGATCTCGCCGCGCTGCGCC
GCGCGCTCGACGCGATCGCGACGGGCTCCGGCGGCGGGCGCGACACCGCGCAGCTCGACTATCAATTCCATCTGCAGATC
GCGCAGTCCACCGGCAACCGCTATTTCGTCGACATCATGACGCAGCTCGGCGCGTCGATCATTCCGCGCACGCGCGTGAA
TTCCGCGCGCTTCGCCGGCGACGATCTCGAGCGCTACGTCGGCCGCCTGAACCACGAGCACGAGGACATCTACGAAGCGA
TCGCGCGCCACGATCCGGAGGCCGCGCGTGCGGCGATGCGCACGCATCTGACCAACAGCCGCGAGCGGCTGCGCCGCGCG
CACGAGGCCGCCGAAGCCGAGCGCGACACGCAGGCGAACTGA

Upstream 100 bases:

>100_bases
CGGATGACTTGTGACGCAGTATAATGAAACCGCGGCTTTCGCTCAAACCCCGCGTAAACCCTCGGCTCACACTACGATCA
TTCAGAAAGGAACCGGCCTC

Downstream 100 bases:

>100_bases
GGCGCGCGCAACGGCGCGGCGCAATCGCGCGTCGTGCCGGGCCGCGCCGCCGGTGCGTCACGCCACAATATCAGTCGTCG
TACAAGATCGGCCGCTTCAC

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLIETRHGIGSFV
LEPSRRQALGIDPATITTLRDVLAVLELRISLESECASLAAQRATDADLAALRRALDAIATGSGGGRDTAQLDYQFHLQI
AQSTGNRYFVDIMTQLGASIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAERDTQAN

Sequences:

>Translated_253_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLIETRHGIGSFV
LEPSRRQALGIDPATITTLRDVLAVLELRISLESECASLAAQRATDADLAALRRALDAIATGSGGGRDTAQLDYQFHLQI
AQSTGNRYFVDIMTQLGASIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAERDTQAN
>Mature_252_residues
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLIETRHGIGSFVL
EPSRRQALGIDPATITTLRDVLAVLELRISLESECASLAAQRATDADLAALRRALDAIATGSGGGRDTAQLDYQFHLQIA
QSTGNRYFVDIMTQLGASIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRAH
EAAEAERDTQAN

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=32.7433628318584, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790780, Length=214, Percent_Identity=33.1775700934579, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790032, Length=236, Percent_Identity=28.3898305084746, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1789353, Length=238, Percent_Identity=27.7310924369748, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994955, Length=224, Percent_Identity=29.0178571428571, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786303, Length=226, Percent_Identity=32.7433628318584, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27654; Mature: 27523

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLIETRHGIGSFVLEPSRRQALGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRATDADLAALRRALDAIATGSGGGRDTAQLDYQFHLQIAQSTGNRYFVDIMTQLGASI
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEECHHEEEEEECCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAERDTQAN
HHHHHHHHCCCCC
>Mature Secondary Structure 
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLIETRHGIGSFVLEPSRRQALGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRATDADLAALRRALDAIATGSGGGRDTAQLDYQFHLQIAQSTGNRYFVDIMTQLGASI
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEECHHEEEEEECCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAERDTQAN
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]