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The map label for this gene is livK [C]

Identifier: 161525310

GI number: 161525310

Start: 2355646

End: 2356794

Strand: Direct

Name: livK [C]

Synonym: Bmul_2140

Alternate gene names: 161525310

Gene position: 2355646-2356794 (Clockwise)

Preceding gene: 161525309

Following gene: 161525312

Centisome position: 68.31

GC content: 65.71

Gene sequence:

>1149_bases
ATGAAACTTCGCCATTCATTGTTGTCCGTGTCGATTGCATCCGCGCTTGCCCTCTTCTCGCAACAGGCCGCGCAGGCGGC
CGACGAACTCGACGTGAAGGTCGGCTTCGCCGCCCCGCTGACCGGCGTGAACGCCGGCTACGGCAAGGACCTGCAGAACG
GCGTGCAGCTCGCGCTCGACGATGCGGTCGCGCAGAAGGTGCAGATCGCCGGCAAGCCCGCGCACTTCAGCCTGATCGTG
CAGGACGATCAGGCCGACCCGCGCGTCGGCGTGCAGGCCGCGCAGGCGCTCGTCGACAAGAACGTGTCGGTCGTGGTCGG
CCACTTCAATTCGGGCACGACGATTCCGGCATCGGTCGTCTACGACAAGGCCGGCATCCCCGTCATCGATCCGGCCGCGA
CGAACCCGACGCTCACGTCGCGCGGGCTCGCCAACATGTTCATGGTGATCGCGACCGACGGCCAGAACGCCGGCAACGCG
GGCAAATACGCGGTCGAGGTGACGAAGGCGAAGCGCATCGCGATCGTCGACGACCGCACCGCATTCGGTCAGGGCGAGGC
CGACGAGTTCGAGAAGGCCGTGAAGGCGGCCGGCGGCACGATCATCGCGCGCGAGTTCACGAACAATCAGGCCGTCGATT
TCCGCGCGCAGATCACGAGCCTGAAGGGCAAGAACCCGGACCTGATCTTCTTCGGCGGCCTCGACGCGCTCGCGGCGAAC
TTCATCAAGCAGATGCGCCAGCTCGGGCTCAATGCACAATTCGTCGGCGGCGGCGGCGTGAAGGACAATGAGTTCATCAA
GATCGCCGGCCCGGCGGCCGAAGGCGCGATGGCGTGGGAGTACGGGCGGCCGCTCGACGAACTGCCGCAGGGCAAGGATT
TCGAGCAGCGGTTCAAGAAGCGCTTCGGCGTCGACGTGCTGTCGTACGCGCAGTTCGGCTACGACGCGACGTGGGCCGCG
ATCAAGGCGATGCAGGCGGCCGGCTCGACCGATCCGAACGTCTATCGGCCGGCGCTGAAGAAGATCGACTTCGAGGGCAT
CACCGGGCGCATCTCGTTCGCGAAGGACGGCTCGCTGAAGAGCGGGATGTCGACGCTGTATCAGGTGAAGAACGGCGCGT
GGAAGACGATCGTCACGAAGGGAGGCTGA

Upstream 100 bases:

>100_bases
CCCTGCGCCCGATTCCGAGAAGCGGTCGAACCGCCCGTCCGCCGCACGCGCCCGGCCGCGTGCGGTGTCCACCCGCCGGT
GTCGAACAAGAGGATCGAAC

Downstream 100 bases:

>100_bases
TCGGGATGGCGGTCCGGGCGACGGGGCGGCGCGGGCCGCCCTAGCACGGGCCAGCCCGCCTGCACGATGCGGTGAAGCGG
CCGATCTTGTACGACGACTG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKLRHSLLSVSIASALALFSQQAAQAADELDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAVAQKVQIAGKPAHFSLIV
QDDQADPRVGVQAAQALVDKNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNA
GKYAVEVTKAKRIAIVDDRTAFGQGEADEFEKAVKAAGGTIIAREFTNNQAVDFRAQITSLKGKNPDLIFFGGLDALAAN
FIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDATWAA
IKAMQAAGSTDPNVYRPALKKIDFEGITGRISFAKDGSLKSGMSTLYQVKNGAWKTIVTKGG

Sequences:

>Translated_382_residues
MKLRHSLLSVSIASALALFSQQAAQAADELDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAVAQKVQIAGKPAHFSLIV
QDDQADPRVGVQAAQALVDKNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNA
GKYAVEVTKAKRIAIVDDRTAFGQGEADEFEKAVKAAGGTIIAREFTNNQAVDFRAQITSLKGKNPDLIFFGGLDALAAN
FIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDATWAA
IKAMQAAGSTDPNVYRPALKKIDFEGITGRISFAKDGSLKSGMSTLYQVKNGAWKTIVTKGG
>Mature_382_residues
MKLRHSLLSVSIASALALFSQQAAQAADELDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAVAQKVQIAGKPAHFSLIV
QDDQADPRVGVQAAQALVDKNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNA
GKYAVEVTKAKRIAIVDDRTAFGQGEADEFEKAVKAAGGTIIAREFTNNQAVDFRAQITSLKGKNPDLIFFGGLDALAAN
FIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKKRFGVDVLSYAQFGYDATWAA
IKAMQAAGSTDPNVYRPALKKIDFEGITGRISFAKDGSLKSGMSTLYQVKNGAWKTIVTKGG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=30.6590257879656, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI48994941, Length=332, Percent_Identity=29.5180722891566, Blast_Score=137, Evalue=9e-34,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40377; Mature: 40377

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRHSLLSVSIASALALFSQQAAQAADELDVKVGFAAPLTGVNAGYGKDLQNGVQLALD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHHEEHHH
DAVAQKVQIAGKPAHFSLIVQDDQADPRVGVQAAQALVDKNVSVVVGHFNSGTTIPASVV
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHEE
YDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAGKYAVEVTKAKRIAIVDDRT
ECCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCEEEEEEECCC
AFGQGEADEFEKAVKAAGGTIIAREFTNNQAVDFRAQITSLKGKNPDLIFFGGLDALAAN
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCEEEECCHHHHHHH
FIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKK
HHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECCCCCHHHCCCCCCHHHHHHH
RFGVDVLSYAQFGYDATWAAIKAMQAAGSTDPNVYRPALKKIDFEGITGRISFAKDGSLK
HHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHH
SGMSTLYQVKNGAWKTIVTKGG
HHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MKLRHSLLSVSIASALALFSQQAAQAADELDVKVGFAAPLTGVNAGYGKDLQNGVQLALD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHHEEHHH
DAVAQKVQIAGKPAHFSLIVQDDQADPRVGVQAAQALVDKNVSVVVGHFNSGTTIPASVV
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHEE
YDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAGKYAVEVTKAKRIAIVDDRT
ECCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCEEEEEEECCC
AFGQGEADEFEKAVKAAGGTIIAREFTNNQAVDFRAQITSLKGKNPDLIFFGGLDALAAN
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCEEEECCHHHHHHH
FIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGRPLDELPQGKDFEQRFKK
HHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECCCCCHHHCCCCCCHHHHHHH
RFGVDVLSYAQFGYDATWAAIKAMQAAGSTDPNVYRPALKKIDFEGITGRISFAKDGSLK
HHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHH
SGMSTLYQVKNGAWKTIVTKGG
HHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA