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The map label for this gene is gudD [H]

Identifier: 161525312

GI number: 161525312

Start: 2358013

End: 2359365

Strand: Direct

Name: gudD [H]

Synonym: Bmul_2142

Alternate gene names: 161525312

Gene position: 2358013-2359365 (Clockwise)

Preceding gene: 161525310

Following gene: 161525316

Centisome position: 68.38

GC content: 69.99

Gene sequence:

>1353_bases
ATGAACGCCGTCACTGCCCCGCCCTCCCCCGATACGCCGCGCATCGTCGACCTGCAAGCGATTCCGGTCGCCGGTCACGA
CAGCATGCTGCTCAACCTGAGCGGCGCGCACGGCCCGTTCTTCACCCGCAACCTCGTGATCCTGAAGGACAGCGCCGGAC
GCACGGGCGTCGGCGAGGTGCCGGGCGGCGAAAGCATCCGCCGCACGCTCGACGATGCGCGCGCGCTCGTCGTGGGCCAG
CCGGTCGGCAACTACCAGGCCGTGCTCAACGCGGTGCGCCGCACGTTCGCGGACCGCGATGCCGGCGGCCGCGGCCTGCA
GACCTTCGATCTGCGCACGACGATCCACGCGGTCACCGCGCTCGAAGCCGCGCTGCTCGACCTGCTCGGCCAGCATCTCG
GCGTGCCGGTCGCGGCGCTGCTCGGCGAAGGCCAGCAACGCGAAAGCGTCGAGATGCTCGGCTATCTGTTCTATGTCGGC
GATCGCACGAAAACCGCGCTGCCGTATCGCGACAGCCGCGACGCGACCGACGACTGGACGCGCGTGCGCAACGAGCCCGC
GCTGACGCCCGAAGCGGTCGTGCGGCTCGCGGAAGCGGCGCACGCGCGCTACGGCTTCAACGATTTCAAGCTCAAAGGCG
GCGTGTTCGAAGGCGCGCGTGAAGTGGAAGCCGTCACGGCGCTCGCCGAACGCTTCCCGAGCGCGCGCGTGACGCTCGAC
CCGAACGGCGCATGGTCGCTCGACGAGGCAGTCCGCCTCTGTCGCGACCTGCATCACGTGCTCGCGTACGCGGAAGACCC
GTGCGGCGCCGAGAACGGCTATTCGGGCCGCGAGGTGATGGCCGAATTCCGCCGCGCGACGGGCCTGCCGACCGCGACCA
ACATGATCGCGACCGACTGGCGGCAGATGGGCCACGCGGTGCAGTTGAACGCGGTCGACATCCCGCTCGCCGATCCGCAC
TTCTGGACCATGCAGGGCTCGGTGCGCGTCGCGCAGATGTGCCGCGACTGGGGGCTGACGTGGGGCTCGCATTCGAACAA
CCACTTCGACGTGTCGCTGGCGATGTTCACGCATGTCGCGGCGGCGGCGCCGGGCCGCGTGACGGCGATCGACACGCACT
GGATCTGGCAGGACGGCGAGCGGCTGACGCGCGAGCCGCTGAAGATCGAGAACGGGCTCGTCGAAGTGCCGAAGCGGCCG
GGGCTCGGTGTCGACATCGACATGGACGCCGTCGCGCGTGCGCACGAGCTGTACAAGCAGCACGGCCTCGGCGCGCGCGA
CGACGCGGCGGCGATGCAGTACCTGATTCCGGGCTGGACCTTCGACAACAAGCGGCCGTGCCTCGTGCGCTGA

Upstream 100 bases:

>100_bases
ACGGCGCCCGCGCACATCGGTTGTCGAATGATCGCACCTCCCGCCGCGCGCGGCAAAGCTTTTGCCGTGCGGCATCGCCC
ACCTTATCGCCATCGCCGCC

Downstream 100 bases:

>100_bases
GCGTCGCGGGACGGAACGGACCGGCGTGAGCGCGTCGCGCGAGCGACACGCGCATCACGCCGCTGCGCGCGGCAAGCGAG
CCGCGCCGCGCATGCGCCGG

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNAVTAPPSPDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYQAVLNAVRRTFADRDAGGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRESVEMLGYLFYVG
DRTKTALPYRDSRDATDDWTRVRNEPALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGAREVEAVTALAERFPSARVTLD
PNGAWSLDEAVRLCRDLHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLNAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGRVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGVDIDMDAVARAHELYKQHGLGARDDAAAMQYLIPGWTFDNKRPCLVR

Sequences:

>Translated_450_residues
MNAVTAPPSPDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYQAVLNAVRRTFADRDAGGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRESVEMLGYLFYVG
DRTKTALPYRDSRDATDDWTRVRNEPALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGAREVEAVTALAERFPSARVTLD
PNGAWSLDEAVRLCRDLHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLNAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGRVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGVDIDMDAVARAHELYKQHGLGARDDAAAMQYLIPGWTFDNKRPCLVR
>Mature_450_residues
MNAVTAPPSPDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEVPGGESIRRTLDDARALVVGQ
PVGNYQAVLNAVRRTFADRDAGGRGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRESVEMLGYLFYVG
DRTKTALPYRDSRDATDDWTRVRNEPALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGAREVEAVTALAERFPSARVTLD
PNGAWSLDEAVRLCRDLHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAVQLNAVDIPLADPH
FWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVAAAAPGRVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRP
GLGVDIDMDAVARAHELYKQHGLGARDDAAAMQYLIPGWTFDNKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=74.5454545454545, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=442, Percent_Identity=64.4796380090498, Blast_Score=567, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49188; Mature: 49188

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVTAPPSPDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEV
CCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCC
PGGESIRRTLDDARALVVGQPVGNYQAVLNAVRRTFADRDAGGRGLQTFDLRTTIHAVTA
CCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
LEAALLDLLGQHLGVPVAALLGEGQQRESVEMLGYLFYVGDRTKTALPYRDSRDATDDWT
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHEEEEEECCCCCEECCCCCCCCCCHHHH
RVRNEPALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGAREVEAVTALAERFPSARVTLD
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
PNGAWSLDEAVRLCRDLHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDW
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQMGHAVQLNAVDIPLADPHFWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVA
HHCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGRVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRPGLGVDIDMDAVARAHELYKQ
HCCCCCEEEEEEEEEECCCHHHHCCCHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHH
HGLGARDDAAAMQYLIPGWTFDNKRPCLVR
HCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure
MNAVTAPPSPDTPRIVDLQAIPVAGHDSMLLNLSGAHGPFFTRNLVILKDSAGRTGVGEV
CCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCC
PGGESIRRTLDDARALVVGQPVGNYQAVLNAVRRTFADRDAGGRGLQTFDLRTTIHAVTA
CCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
LEAALLDLLGQHLGVPVAALLGEGQQRESVEMLGYLFYVGDRTKTALPYRDSRDATDDWT
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHEEEEEECCCCCEECCCCCCCCCCHHHH
RVRNEPALTPEAVVRLAEAAHARYGFNDFKLKGGVFEGAREVEAVTALAERFPSARVTLD
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
PNGAWSLDEAVRLCRDLHHVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDW
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQMGHAVQLNAVDIPLADPHFWTMQGSVRVAQMCRDWGLTWGSHSNNHFDVSLAMFTHVA
HHCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGRVTAIDTHWIWQDGERLTREPLKIENGLVEVPKRPGLGVDIDMDAVARAHELYKQ
HCCCCCEEEEEEEEEECCCHHHHCCCHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHH
HGLGARDDAAAMQYLIPGWTFDNKRPCLVR
HCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]