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The map label for this gene is purR [H]

Identifier: 161525243

GI number: 161525243

Start: 2268128

End: 2269153

Strand: Direct

Name: purR [H]

Synonym: Bmul_2073

Alternate gene names: 161525243

Gene position: 2268128-2269153 (Clockwise)

Preceding gene: 161525242

Following gene: 161525244

Centisome position: 65.77

GC content: 70.18

Gene sequence:

>1026_bases
ATGACCTCGACCATCAAGGATGTCGCCGCACTCGCCGGCTTTTCGATCGCCACCGTGTCGCGCGCGATCAATGCGCCGCA
TACCGTCAGTCCGGCGACGCTCGAAGCGATCCGCGCGGCGATCGATACGCTGCGCTTTCGTCCGAGTCCGCTCGGCCGGC
AACTGCGCGGCGAGCGCACGCGGCTCGTCGGCGTCGTCGTGCCGACGCTGTCGAATCCCGTATTCGCCGACTGCCTGCTC
GGCATCGACGAGCTCGCGACCGCGGCCGGTTTCAAGCTGATCCTGATGACGACCGAATACGACGCGGCGCGCGAACGTCA
CGCGATCGAGACGCTGCGCGAGCAGCGCGTCGAAGGCCTGATCCTGACCGTCGCCGACGCCGACACGCATCCGCTGCTCG
ACATGCTCGATCGCGACGGACCGCATTACGTGCTGATGCACAACGACACGCAGCGCCGGCCGTCGGTGTCCGTCGACAAC
CGGCACGCCGCATACGACGGCGTGCGGCTGCTCACCGCGCGCGGCCATCGCCGCGTGCTGATGCTCGCGGGCTCGCTCGC
CGCATCCGATCGCGCGCGCCAGCGTCATCTCGGCTATGCGCAGGCGCTCGCCGAAAGCGGCGTCGCGACGCTGCCGCCCG
TCGAAGTCGACTTCAACGCGCCCGAACTGCCCGACGCGGTGCTCGCGCATCTGACCGCGCACGCGACGCGTCCGACCGCC
CTCTTCTGCAGCAACGACCTGCTCGCGATGGTCGTGATGCGCGGGCTGCGCCGCGCCGGTTTCTCGATCCCGGACGACCT
GTCGGTACTCGGCTTCGACGGCATCGACATCGGCGAGCTACTCGCGCCGCCGCTCGCGAGCGTCGCGACGCCGAACCGCG
ACATCGGCCGCCATGCCTGGCGACGATTGATCGACGCGATCGGCGGCGCCGCGATCGAACGCACGTCGCTGATCCTGCCG
CACGCGGTGCGCGACGGCGCGACCGTCGCCCCGCCCGCGGCAGACGTGCAACTGCGTCGCGCGTAA

Upstream 100 bases:

>100_bases
CGCAGACGCACACCGCGATACGCCCCCTTCACGCGTCATCGACAGGTCACAAACGTTTTCGATAATCAACCCGCCGAAAA
CGTTTACATCGCGTGACCTC

Downstream 100 bases:

>100_bases
ACCCGCGCACCGTGCGCCGCGTTCTTCATGCGGCGCGCGGCGACGTCCCCTTTCTTCGTCCGGAGACCTGATCGTGCCTT
TCCGTCTGACCTCGCTGCTG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLEAIRAAIDTLRFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLL
GIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDN
RHAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALAESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIDIGELLAPPLASVATPNRDIGRHAWRRLIDAIGGAAIERTSLILP
HAVRDGATVAPPAADVQLRRA

Sequences:

>Translated_341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLEAIRAAIDTLRFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLL
GIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDN
RHAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALAESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIDIGELLAPPLASVATPNRDIGRHAWRRLIDAIGGAAIERTSLILP
HAVRDGATVAPPAADVQLRRA
>Mature_340_residues
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLEAIRAAIDTLRFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLLG
IDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNR
HAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALAESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTAL
FCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIDIGELLAPPLASVATPNRDIGRHAWRRLIDAIGGAAIERTSLILPH
AVRDGATVAPPAADVQLRRA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=321, Percent_Identity=30.2180685358255, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=31.547619047619, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=32.3232323232323, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994940, Length=335, Percent_Identity=28.3582089552239, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790715, Length=345, Percent_Identity=26.6666666666667, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787948, Length=312, Percent_Identity=29.1666666666667, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788474, Length=300, Percent_Identity=31.3333333333333, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=27.8787878787879, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.4797507788162, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787906, Length=334, Percent_Identity=26.3473053892216, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=24.1486068111455, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=26.2295081967213, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36657; Mature: 36525

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLEAIRAAIDTLRFRPSPLGRQLRGERT
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLLGIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCHHHHHHHHCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNRHAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALAESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIDIGELLAPPLASVATPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCHHHHCCCCCHHHHHHH
RRLIDAIGGAAIERTSLILPHAVRDGATVAPPAADVQLRRA
HHHHHHHCCCHHHHHHEECCHHHCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLEAIRAAIDTLRFRPSPLGRQLRGERT
CCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLLGIDELATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCHHHHHHHHCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNRHAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALAESGVATLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIDIGELLAPPLASVATPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCHHHHCCCCCHHHHHHH
RRLIDAIGGAAIERTSLILPHAVRDGATVAPPAADVQLRRA
HHHHHHHCCCHHHHHHEECCHHHCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA