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The map label for this gene is proP [H]

Identifier: 161525208

GI number: 161525208

Start: 2233507

End: 2235090

Strand: Direct

Name: proP [H]

Synonym: Bmul_2038

Alternate gene names: 161525208

Gene position: 2233507-2235090 (Clockwise)

Preceding gene: 161525206

Following gene: 161525211

Centisome position: 64.77

GC content: 67.3

Gene sequence:

>1584_bases
ATGGCCTGCCCCGACGCGCAGCACACGACATCGCGCTCGCCGGCAAACCGTGTCGGACGGCCCGCCGTTCCGGCGCAGTC
AAAGGAGTGCATGACCTTGACCGCAACACACGTCAGCCCGACCGCTGTTTCTTCCACCTCGTCGTCCACGGAGGCCCCGC
TCGCCGCGGACGACATTACCGTCGTCGATCAAAGCCTGCTCAAGCGTGCCGTCAGCGCCATGGCGCTCGGCAACGCGATG
GAATGGTTCGACTTCGGCGTCTACAGCTATATCGCCGTCACGCTCGGCAAAGTGTTCTTCCCGTCCAGCAGCCCGTCCGC
GCAGCTGCTGGCCACTTTCGGCACGTTCGCGGCCGCGTTCCTCGTGCGCCCGCTCGGCGGCATGGTGTTCGGCCCGCTCG
GCGATCGCATCGGCCGTCAGCGCGTGCTCGCGATGACGATGATCATGATGGCCGTCGGCACGTTCGCGATCGGCCTGATT
CCGAGCTACGCGTCGATCGGCATCATGGCGCCCGTGCTGCTGCTCGTCGCGCGTCTCGTGCAGGGCTTCTCGACCGGCGG
CGAATACGGCGGCGCGGCGACCTTCATCGCCGAATTCTCGACCGACAAGCGCCGCGGCTTCATGGGCAGCTTCCTCGAAT
TCGGTACGCTGATCGGCTATGTGATGGGTGCGGGCGTCGTCGCGCTGCTCACCGCGTCGCTGTCGCAGGAAGCGCTGCTG
TCGTGGGGCTGGCGCGTGCCGTTCCTGATCGCGGGTCCGCTCGGCCTGATCGGTCTCTATATCCGGATGAAGCTCGAGGA
AACGCCGGCGTTCAAGCGCCAGGCCGAGGAGCGCGAAGCGCAGGACAAGGCCGTGCCGAAGGCGCGTTTCCGCGAAACGC
TGCTGCGCAACTGGCGCGCGCTGCTGCTCTGCGTCGGCCTCGTGCTGATCTTCAACGTGACCGACTACATGGTGCTGTCG
TATCTGCCGAGCTTCATGTCGTCGACGCTGCACTTCGACGAATCGCACAGCCTCGTGCTCGTGCTGATCGTGATGGTGCT
GATGATGCCGATGACGCTCGCGGCCGGCCGTCTGTCCGACCACGTCGGCCGCAAGCCGGTGATGCTCGCCGGCTGCGTCG
GGCTGCTCGTGCTCGCGATTCCGTCGATGCTGCTGATCCATGCGGGAACGACCGCCTCGGTGTTCGGCGGCCTGCTGATC
CTCGGCGTGCTGCTGTCGTGCTTCACGGGCGTGATGCCGTCGGCGCTGCCCGCGCTGTTCCCGACCGAGATCCGCTACGG
TGCGCTCGCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTGATGACCGCATGGCTCGTCGACG
TCACGCACAACCTGATGATGCCGGCCTACTACATGATGGGCGCCGCCGTGATCGGCATCGTGTCGGTCGTCGCGCTCGCC
GAGACCGCGCGCAAGCCGCTGAAGGGCTCGCCGCCCGCCGTCGCGACGCATCGCGAAGCGCACCAGCTCGTGCGCCGGCT
GCGCGACGCGGACGAGAACGACGACGCCGACGTGTATCGCGTCGCGTCCACGGCGCGCGCATAA

Upstream 100 bases:

>100_bases
CATCTGCCGCTCGCATCACGCGGCCCATTCTTCATGAGCGCACTGTCGCGACGGCCCAACGGGCCCGCGTCGAAGCAAGC
CCTTGCGTACTAAGCGCTTA

Downstream 100 bases:

>100_bases
GCGACGCGTCGCGCGCTCGCGAAGCGCTGCAACGAAAAACCCCGGTCGAAACCGGGGCTTGATCGGACGGCCGGCATGCC
CGGCCCTGAAACGCCCGACC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MACPDAQHTTSRSPANRVGRPAVPAQSKECMTLTATHVSPTAVSSTSSSTEAPLAADDITVVDQSLLKRAVSAMALGNAM
EWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLI
PSYASIGIMAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALL
SWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLS
YLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLAAGRLSDHVGRKPVMLAGCVGLLVLAIPSMLLIHAGTTASVFGGLLI
LGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLMTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALA
ETARKPLKGSPPAVATHREAHQLVRRLRDADENDDADVYRVASTARA

Sequences:

>Translated_527_residues
MACPDAQHTTSRSPANRVGRPAVPAQSKECMTLTATHVSPTAVSSTSSSTEAPLAADDITVVDQSLLKRAVSAMALGNAM
EWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLI
PSYASIGIMAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALL
SWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLS
YLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLAAGRLSDHVGRKPVMLAGCVGLLVLAIPSMLLIHAGTTASVFGGLLI
LGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLMTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALA
ETARKPLKGSPPAVATHREAHQLVRRLRDADENDDADVYRVASTARA
>Mature_526_residues
ACPDAQHTTSRSPANRVGRPAVPAQSKECMTLTATHVSPTAVSSTSSSTEAPLAADDITVVDQSLLKRAVSAMALGNAME
WFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIP
SYASIGIMAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALLS
WGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSY
LPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLAAGRLSDHVGRKPVMLAGCVGLLVLAIPSMLLIHAGTTASVFGGLLIL
GVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLMTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALAE
TARKPLKGSPPAVATHREAHQLVRRLRDADENDDADVYRVASTARA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=471, Percent_Identity=51.1677282377919, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=30.9302325581395, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=33.9152119700748, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=32.8638497652582, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082231, Length=430, Percent_Identity=23.7209302325581, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=23.013698630137, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323512, Length=450, Percent_Identity=23.7777777777778, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 56184; Mature: 56053

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACPDAQHTTSRSPANRVGRPAVPAQSKECMTLTATHVSPTAVSSTSSSTEAPLAADDIT
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
VVDQSLLKRAVSAMALGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYASIGIMAPVLLLVARLV
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALL
HHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRA
HCCCCCCHHHHCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLAAGRLSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVGRKPVMLAGCVGLLVLAIPSMLLIHAGTTASVFGGLLILGVLLSCFTGVMPSALPALF
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PTEIRYGALAIGFNVSVSLFGGTTPLMTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALA
CHHHCCCEEEEECEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETARKPLKGSPPAVATHREAHQLVRRLRDADENDDADVYRVASTARA
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ACPDAQHTTSRSPANRVGRPAVPAQSKECMTLTATHVSPTAVSSTSSSTEAPLAADDIT
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHH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HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]