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The map label for this gene is gloB [H]

Identifier: 161525203

GI number: 161525203

Start: 2229866

End: 2230672

Strand: Reverse

Name: gloB [H]

Synonym: Bmul_2033

Alternate gene names: 161525203

Gene position: 2230672-2229866 (Counterclockwise)

Preceding gene: 161525207

Following gene: 161525202

Centisome position: 64.69

GC content: 69.52

Gene sequence:

>807_bases
ATGAACGAGCTGGAATACGTGCCGGTGCCGGCATTCGAAGACAACTACATCTGGCTCGTGTCGGACGGTCGCGATGCGAT
CGCCGTCGATCCGGGTGAAGCCGAGCCGGTGCGCCGGGTTCTTGCCGAACGAGGCTGGCGGTTGACCGCTATTCTACTCA
CGCACCATCACGCCGACCATGTCGGCGGTGTCGCGGCACTGCAGGATAGCCAGCCGGCCGATGTGCCGTTGCCCGTGTAC
GGCCCGGCCGACGAGGCGATCGGCGTGGTCACGCATCCGCTCGCGGGCGGCGCGCGCGTGACGCTCGATGCGCCGGCCGC
GACGTTCGACGTGCTGGCCGTGCCCGGTCACACGCGCGGCCATATCGCGTACTTCCAGCCCGCCGGTCCGCACACGCCGG
TGCCGCACGTGTTCTGCGGCGACACGCTGTTCTCGTGCGGCTGCGGCCGCCTGTTCGAAGGCACGCCCGCGCAGATGCTT
GCGTCGCTCGACGCGCTCGCGGCGCTGCCGGGCGACACGCGCGTGCACTGCGCACACGAATACACGCTGTCGAACATCCG
TTTCGCGCTCGCGTGCGAACCCGGCAACGCGGCGCTCGCTGCCTGGCGCGACGAAGCGCAGGCGCTGCGCGCGCGCGGCG
TACCGACGCTGCCGACGACGATCGCACACGAGCGCGCCGTGAACCCGTTCCTGCGTGCCGACGCCGCCGCGATCCACGCG
ACGCTCGAAGCCGAATTGCACGAATCGGTGCCCGATCGTTTGGCCGCCTTCACGCTGATGCGCGAGTGGAAGAACCGGTT
CAGATGA

Upstream 100 bases:

>100_bases
GCGCCTGCTCCCAGCCCAGCACGTAGCGGCCGGGCGGCGAGTCGGTCCAGGCGGGCCAGTCTATAATTTCTCGGTCAGAC
ATAACGATGGTTGCGCGCCC

Downstream 100 bases:

>100_bases
GCGATTCGGGAGGACTCCCGAAGCTCAAGCGGAGTCTGTAAAAATTGCTCCAAACTCAGGATTTCAATGACTTTTCGGTG
TTTTTATTGACGTGAAGCAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAEPVRRVLAERGWRLTAILLTHHHADHVGGVAALQDSQPADVPLPVY
GPADEAIGVVTHPLAGGARVTLDAPAATFDVLAVPGHTRGHIAYFQPAGPHTPVPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDEAQALRARGVPTLPTTIAHERAVNPFLRADAAAIHA
TLEAELHESVPDRLAAFTLMREWKNRFR

Sequences:

>Translated_268_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAEPVRRVLAERGWRLTAILLTHHHADHVGGVAALQDSQPADVPLPVY
GPADEAIGVVTHPLAGGARVTLDAPAATFDVLAVPGHTRGHIAYFQPAGPHTPVPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDEAQALRARGVPTLPTTIAHERAVNPFLRADAAAIHA
TLEAELHESVPDRLAAFTLMREWKNRFR
>Mature_268_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAEPVRRVLAERGWRLTAILLTHHHADHVGGVAALQDSQPADVPLPVY
GPADEAIGVVTHPLAGGARVTLDAPAATFDVLAVPGHTRGHIAYFQPAGPHTPVPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDEAQALRARGVPTLPTTIAHERAVNPFLRADAAAIHA
TLEAELHESVPDRLAAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=268, Percent_Identity=39.5522388059701, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI94538322, Length=268, Percent_Identity=39.5522388059701, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI116642887, Length=244, Percent_Identity=36.8852459016393, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=40.7079646017699, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI21703352, Length=244, Percent_Identity=36.8852459016393, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI46361987, Length=190, Percent_Identity=41.0526315789474, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=45.8015267175573, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17536925, Length=260, Percent_Identity=34.6153846153846, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=266, Percent_Identity=30.0751879699248, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=245, Percent_Identity=31.0204081632653, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356335, Length=239, Percent_Identity=39.7489539748954, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24667711, Length=240, Percent_Identity=39.5833333333333, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667703, Length=239, Percent_Identity=39.7489539748954, Blast_Score=160, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28810; Mature: 28810

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAEPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVAALQDSQPADVPLPVYGPADEAIGVVTHPLAGGARVTLDAPAATFDVLAVPGHTRG
CCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEECCCCCCC
HIAYFQPAGPHTPVPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPGNAALAAWRDEAQALRARGVPTLPTTIAHERAVNPFLRADAAAIHA
EEECCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TLEAELHESVPDRLAAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAEPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVAALQDSQPADVPLPVYGPADEAIGVVTHPLAGGARVTLDAPAATFDVLAVPGHTRG
CCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEECCCCCCC
HIAYFQPAGPHTPVPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPGNAALAAWRDEAQALRARGVPTLPTTIAHERAVNPFLRADAAAIHA
EEECCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TLEAELHESVPDRLAAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA