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The map label for this gene is mltD [H]

Identifier: 161525202

GI number: 161525202

Start: 2228127

End: 2229713

Strand: Reverse

Name: mltD [H]

Synonym: Bmul_2032

Alternate gene names: 161525202

Gene position: 2229713-2228127 (Counterclockwise)

Preceding gene: 161525203

Following gene: 161525201

Centisome position: 64.66

GC content: 67.23

Gene sequence:

>1587_bases
ATGCGATTCATATTGAGCGCGCTGATGGTCCTGCTGCTCGCCGCTTGTGCGAGCCAGGGCCCTGTCGCCAACAACGCCGC
CGATGCACAGGCGACGTCCAACTACCTCCGCAAGTCAGCCACCGCCAAAGAAACCGTCGACGTCGACAAACAATCGGTCG
GCGACCTCACCAGCGCAGACAGCGACCTGTGGGCGCGCATCCGCCGCGGCTTCCAGATGCCCGACCTGCAGAGCGACCTC
GTCGACATGCAGACGACCTGGTACACGCAGCGTCCCGACTACGTGCAGCGCATGACCGAGCGCTCGCAGAAGTACCTCTA
TCACATCGTCGAGGAACTCGAGGCGCGTCACATGCCGACCGAGCTCGCGCTGCTGCCGTTCATCGAGTCCGCGTACAACC
CGCAGGCGCTGTCGGTCGCGAAGGCGGCCGGCATGTGGCAGTTCATGCCGGGCACGGGCCGCACCTACAACCTGAAGCGC
AACATGTGGCAGGACGAGCGGCGCGACGTGCTCGCGTCGACGAGCGCCGCGCTCGACTATCTGTCGCGCCTGCATGACAT
GTTCGGCGACTGGTATCTCGCGCTCGCCGCGTACAACTGGGGCGAGGGCAACGTGCAGCGCGCGATAGCGCGCAATCAGG
CGGCCGGTCTGCCGACCGACTACCAGAGCCTCAGGATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAGGCGGTG
AAGAACATCATCGCGAATCCGCAGCAGTACGGGCTCGCGTTGCCCGACATTCCGAACCACCCGTATTTCGTGACGGTGAC
GACGTCGCACGACATCGACGTGGCCGTGGCCGCGAAGCTCGCGAACCTGTCGCTCGACGAATTCAAGTCGCTGAACCCGT
CGTTCTCGAAGCCCGTGATTCTCGGCGCGACCGAGCCGCAGATCCTGCTGCCGTTCGACAACGCGGCCGCGTTCGAGAAG
AACCTGAAGTCGTATTCGGGCCAGCTGTCGTCGTGGACCACCTATACGGTCAGCGAACGCGCGCGTCCGGCGGCGATCGC
CGAAAAGATCGGCGTCGACGCCGATACGCTGATGGCCGTCAACAAGATTCCGGCCGGCATGCGGCTGAAGCCGGGCTCGA
CGATCGTCGTGCCGCGCGGCGACGACGATGACGAGGACATCAGCGCGGACGTCGCGGAAAACGGCGTGCTGGCGATGGAG
CCCGACGTGCCCGATACGCGCAAGATGCTGATCCGCGTGCGCCGCAAGCAGTCGATGGCGACGATCGCGGGCCGCTACGG
CGTGTCGGTTTCGCAGCTGAAGGCATGGAACCGGACGCGCCGCGACCTCGTGCTGCCGGGCCAGGCGGTCGTGCTGCACG
TGCCGGTCGGCCGCGCCGTGCCGGCCGAGCCGGGCCCCGAGCGGATCGCGACGTCGGTCGGCGGCGGCCGCATCGAGCGC
GCGAGCCTGTCGACGGGCAGCAAGTCGGCGCGCGGCAAGCATGTCGCGAAGCCGGCGGCGCGTGCGACGAAGGCGGCGCC
CGCCAAGGCCGAACGCGCGAAGGCCGCGCCGGCGAAAACGGCCGCGCACAAGGCGAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
AACTCAGGATTTCAATGACTTTTCGGTGTTTTTATTGACGTGAAGCACGCACTTCCGTAGTATCGCCAGCAATTTCCAGC
CGTCGGAAGCCGAGATTTTC

Downstream 100 bases:

>100_bases
GCGACGCACTCGCGCGCGAGCCGGCTTGCGGCGCGCGACTGCGCTATCATCCGACGAAACGCCGTCACCGAGGTGACGGC
GTTTTCGTTTGCGTGCGGCA

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MRFILSALMVLLLAACASQGPVANNAADAQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIANPQQYGLALPDIPNHPYFVTVTTSHDIDVAVAAKLANLSLDEFKSLNPSFSKPVILGATEPQILLPFDNAAAFEK
NLKSYSGQLSSWTTYTVSERARPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMATIAGRYGVSVSQLKAWNRTRRDLVLPGQAVVLHVPVGRAVPAEPGPERIATSVGGGRIER
ASLSTGSKSARGKHVAKPAARATKAAPAKAERAKAAPAKTAAHKAKKK

Sequences:

>Translated_528_residues
MRFILSALMVLLLAACASQGPVANNAADAQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIANPQQYGLALPDIPNHPYFVTVTTSHDIDVAVAAKLANLSLDEFKSLNPSFSKPVILGATEPQILLPFDNAAAFEK
NLKSYSGQLSSWTTYTVSERARPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMATIAGRYGVSVSQLKAWNRTRRDLVLPGQAVVLHVPVGRAVPAEPGPERIATSVGGGRIER
ASLSTGSKSARGKHVAKPAARATKAAPAKAERAKAAPAKTAAHKAKKK
>Mature_528_residues
MRFILSALMVLLLAACASQGPVANNAADAQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIANPQQYGLALPDIPNHPYFVTVTTSHDIDVAVAAKLANLSLDEFKSLNPSFSKPVILGATEPQILLPFDNAAAFEK
NLKSYSGQLSSWTTYTVSERARPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMATIAGRYGVSVSQLKAWNRTRRDLVLPGQAVVLHVPVGRAVPAEPGPERIATSVGGGRIER
ASLSTGSKSARGKHVAKPAARATKAAPAKAERAKAAPAKTAAHKAKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=462, Percent_Identity=34.6320346320346, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57797; Mature: 57797

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFILSALMVLLLAACASQGPVANNAADAQATSNYLRKSATAKETVDVDKQSVGDLTSAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCC
SDLWARIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHH
KNIIANPQQYGLALPDIPNHPYFVTVTTSHDIDVAVAAKLANLSLDEFKSLNPSFSKPVI
HHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNAAAFEKNLKSYSGQLSSWTTYTVSERARPAAIAEKIGVDADTLMAV
EECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
TIAGRYGVSVSQLKAWNRTRRDLVLPGQAVVLHVPVGRAVPAEPGPERIATSVGGGRIER
HHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEE
ASLSTGSKSARGKHVAKPAARATKAAPAKAERAKAAPAKTAAHKAKKK
CCCCCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MRFILSALMVLLLAACASQGPVANNAADAQATSNYLRKSATAKETVDVDKQSVGDLTSAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCC
SDLWARIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHH
KNIIANPQQYGLALPDIPNHPYFVTVTTSHDIDVAVAAKLANLSLDEFKSLNPSFSKPVI
HHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNAAAFEKNLKSYSGQLSSWTTYTVSERARPAAIAEKIGVDADTLMAV
EECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
TIAGRYGVSVSQLKAWNRTRRDLVLPGQAVVLHVPVGRAVPAEPGPERIATSVGGGRIER
HHHHHCCCCHHHHHHHHHCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEE
ASLSTGSKSARGKHVAKPAARATKAAPAKAERAKAAPAKTAAHKAKKK
CCCCCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]