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The map label for this gene is recE [H]

Identifier: 161525023

GI number: 161525023

Start: 2023385

End: 2024197

Strand: Direct

Name: recE [H]

Synonym: Bmul_1850

Alternate gene names: 161525023

Gene position: 2023385-2024197 (Clockwise)

Preceding gene: 161525022

Following gene: 161525024

Centisome position: 58.67

GC content: 64.21

Gene sequence:

>813_bases
ATGCTGATCGAACATCTCGACATCGACGAATACCACGCGCGGCCAGAGGTCTCGAAGTCGCAGCTCGACACGCTCGACCT
GTCGCCCGCGCACTTCTGGGCGCTGCATCGCGATCCGCAACGGCCCGCGCCAACCACGCGCGGCGGCCAGCTCGAAGGCC
AGCTCGCACACTGCGCGATTCTCGAGCCCGAAGAGTTCGACAAGCGTTACGTGCTCGGCCCGACCGTGAACCGCAATACG
AAGGTCTGGAAGGAGTTCGTCGACGAGAACGCCGGCCGCATCGCGATCCAGCAGGACCAGTACGACACCGCGTGGCGCCA
GTCGGACGCCGTGCGCGCGCTGCCGGAGATACGCGAGGCGTTGTCGCGTGGCCGCGCCGAAGTGTCGGCGTTCTGGACCG
ACGAGGAAACCGGCGTCGAGTGCCGATGCCGACCTGACTGGGTACACGACTGCGGCGACGCCGGCGTGATCCTGCTCGAC
CTCAAGACGTACAGCATCGCGAGCCCTGGTGAGTTTCGCCGGCAGGTCGCAAGAAAACGCTACGACGTGCAGGCCGCGCT
CTACAGCGATGGCTATGCGAAGGCATCAGGCCGCCCTGTGCTCGGCTTCGTGTTCGTTGCTGTCGAGACGGAATACCCGT
TCGCCGCGAACGCGTTAATGCTCGACGAAGAAAGCCTCGAATCTGGGCGCACGAAGTATCGCAAGAACCTGCGCACGTAC
GCCGAGTGCATGCGCACCAACACGTGGCCTGGGTATTCGACGGGCATTGACATCATCCGCCTGCCGCAATGGGCGCTCAT
CACCGAGGAATAA

Upstream 100 bases:

>100_bases
CCGGGATTCTCGTTGCGATGGCAATCGCGTTTGCATGCGCGATCGGCATTGCTTCGATCCCGGATTCCACGCTCGCGGTG
CGCGCAGCTTGGGGGACGTA

Downstream 100 bases:

>100_bases
AAACCATGGGTCAAACCACCAACATCGCCAAGCTCAAGCAGACCTCCAAGATGGTCGCGCGCGATGCCGGTATCGGCAGC
GTCAAGACCTTCTTCGAGTC

Product: exonuclease VIII, 5' -> 3' specific dsDNA exonuclease

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLIEHLDIDEYHARPEVSKSQLDTLDLSPAHFWALHRDPQRPAPTTRGGQLEGQLAHCAILEPEEFDKRYVLGPTVNRNT
KVWKEFVDENAGRIAIQQDQYDTAWRQSDAVRALPEIREALSRGRAEVSAFWTDEETGVECRCRPDWVHDCGDAGVILLD
LKTYSIASPGEFRRQVARKRYDVQAALYSDGYAKASGRPVLGFVFVAVETEYPFAANALMLDEESLESGRTKYRKNLRTY
AECMRTNTWPGYSTGIDIIRLPQWALITEE

Sequences:

>Translated_270_residues
MLIEHLDIDEYHARPEVSKSQLDTLDLSPAHFWALHRDPQRPAPTTRGGQLEGQLAHCAILEPEEFDKRYVLGPTVNRNT
KVWKEFVDENAGRIAIQQDQYDTAWRQSDAVRALPEIREALSRGRAEVSAFWTDEETGVECRCRPDWVHDCGDAGVILLD
LKTYSIASPGEFRRQVARKRYDVQAALYSDGYAKASGRPVLGFVFVAVETEYPFAANALMLDEESLESGRTKYRKNLRTY
AECMRTNTWPGYSTGIDIIRLPQWALITEE
>Mature_270_residues
MLIEHLDIDEYHARPEVSKSQLDTLDLSPAHFWALHRDPQRPAPTTRGGQLEGQLAHCAILEPEEFDKRYVLGPTVNRNT
KVWKEFVDENAGRIAIQQDQYDTAWRQSDAVRALPEIREALSRGRAEVSAFWTDEETGVECRCRPDWVHDCGDAGVILLD
LKTYSIASPGEFRRQVARKRYDVQAALYSDGYAKASGRPVLGFVFVAVETEYPFAANALMLDEESLESGRTKYRKNLRTY
AECMRTNTWPGYSTGIDIIRLPQWALITEE

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=277, Percent_Identity=35.3790613718412, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 30796; Mature: 30796

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEHLDIDEYHARPEVSKSQLDTLDLSPAHFWALHRDPQRPAPTTRGGQLEGQLAHCAI
CCCCCCCCHHHHCCCCCCHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEE
LEPEEFDKRYVLGPTVNRNTKVWKEFVDENAGRIAIQQDQYDTAWRQSDAVRALPEIREA
ECCHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHCHHHHHHHHHHHH
LSRGRAEVSAFWTDEETGVECRCRPDWVHDCGDAGVILLDLKTYSIASPGEFRRQVARKR
HHCCCCEEEEEECCCCCCCEEEECCCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
YDVQAALYSDGYAKASGRPVLGFVFVAVETEYPFAANALMLDEESLESGRTKYRKNLRTY
HHHEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
AECMRTNTWPGYSTGIDIIRLPQWALITEE
HHHHHCCCCCCCCCCCEEEECCCEEEEECC
>Mature Secondary Structure
MLIEHLDIDEYHARPEVSKSQLDTLDLSPAHFWALHRDPQRPAPTTRGGQLEGQLAHCAI
CCCCCCCCHHHHCCCCCCHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEE
LEPEEFDKRYVLGPTVNRNTKVWKEFVDENAGRIAIQQDQYDTAWRQSDAVRALPEIREA
ECCHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHCHHHHHHHHHHHH
LSRGRAEVSAFWTDEETGVECRCRPDWVHDCGDAGVILLDLKTYSIASPGEFRRQVARKR
HHCCCCEEEEEECCCCCCCEEEECCCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
YDVQAALYSDGYAKASGRPVLGFVFVAVETEYPFAANALMLDEESLESGRTKYRKNLRTY
HHHEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
AECMRTNTWPGYSTGIDIIRLPQWALITEE
HHHHHCCCCCCCCCCCEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]