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The map label for this gene is rbsB [H]

Identifier: 161524886

GI number: 161524886

Start: 1892760

End: 1893704

Strand: Direct

Name: rbsB [H]

Synonym: Bmul_1713

Alternate gene names: 161524886

Gene position: 1892760-1893704 (Clockwise)

Preceding gene: 161524885

Following gene: 161524887

Centisome position: 54.89

GC content: 64.76

Gene sequence:

>945_bases
ATGTTCAAGCACAAAGCCGCCCTGACCGCCCTCGCGTGCGCGTTCGCCTTCGGCGCATCGGCCGCGCATGCGGCCGACAA
GCCGCTGAAGCTGATCGGCGTCACGGTCGGCTCGCTCGGCAACCCGTATTTCGTCACGATCGTGAAGAGCGCCGAAGCGC
GCGCGAAACAGATCAACCCGAACGCGAAGGTCACCGCTGTTTCGGCCGACTACGACCTGAACAAGCAGTTCACGCAGATC
GACAACTTCATCTCCGCGCACGTCGACATGATCCTGCTGAACGCGACCGATCCGAAGGCGATCGAGCCGGCGGTCAAGAA
GGCGCAGGCGGCCGGCATCACGGTCGTCGCGGTCGACGTCGCCGCGGCCGGCGCGAACGCGACCGTGCAGACGAACAACG
TGAAGGCCGGCGAACTCGCGTGCGACTACATCGCGAAGAAACTCAACGGCAAGGGCAACGTGATCATCGAGAACGGCCCG
CAGGTGTCGGCCGTGATCGATCGCGTGAACGGCTGCAAGGCCGTGCTCGCGAAGAGCCCCGGCATCAAGCTGCTGTCGAG
CGACCAGGACGGCAAGGGCTCGCGCGAAGGCGGGATGAACGCGATGCAAGGCTATCTGACGCGCTTTCCGAAACTCGACG
CGGTGTTCACGATCAACGATCCGCAGGCGATCGGCAGCGATCTCGCCGCGAAGCAGCTGAACCGTTCGGGGATCGTGATC
ACGTCGGTCGACGGCGCACCCGACATCGAGGTCGCGCTGAAGTCGAACACGCTCGTTCAGGCGTCCGCGAGCCAGGATCC
GTGGGCGATGGCGCAGCAGGCCGTCAACGTCGGCTACGGGATCATGAACGGCCAGAAGCCGGCGAATCCGATGATCCTGA
TCGAGCCGACGCTCGTCACGCGCGACAACGTGAAGAGCTACAAGGGCTGGAGCACGCCGCGCTGA

Upstream 100 bases:

>100_bases
GACTCGGCGCGCACCTGACGCGCGGCTCGCCGCTTTCCTCTGCGCGCAGGCAGGTCAACCTGCGCGCGTTCGCCAACAGA
CCTCACGGAGACACAACGAC

Downstream 100 bases:

>100_bases
GCGCCCTGCTTCACGCGCCGCGCCGACGATGCACGCGCGACGCCGCCCCGCGTCGTCGGCACGGCCCACCCGTTCGTTTT
TTCCGCGGCACCGCCCGGCC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFKHKAALTALACAFAFGASAAHAADKPLKLIGVTVGSLGNPYFVTIVKSAEARAKQINPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYIAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKSPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDAVFTINDPQAIGSDLAAKQLNRSGIVI
TSVDGAPDIEVALKSNTLVQASASQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLVTRDNVKSYKGWSTPR

Sequences:

>Translated_314_residues
MFKHKAALTALACAFAFGASAAHAADKPLKLIGVTVGSLGNPYFVTIVKSAEARAKQINPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYIAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKSPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDAVFTINDPQAIGSDLAAKQLNRSGIVI
TSVDGAPDIEVALKSNTLVQASASQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLVTRDNVKSYKGWSTPR
>Mature_314_residues
MFKHKAALTALACAFAFGASAAHAADKPLKLIGVTVGSLGNPYFVTIVKSAEARAKQINPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYIAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKSPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDAVFTINDPQAIGSDLAAKQLNRSGIVI
TSVDGAPDIEVALKSNTLVQASASQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLVTRDNVKSYKGWSTPR

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=284, Percent_Identity=29.9295774647887, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790674, Length=191, Percent_Identity=28.2722513089005, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32800; Mature: 32800

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHKAALTALACAFAFGASAAHAADKPLKLIGVTVGSLGNPYFVTIVKSAEARAKQINP
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCHHHHHCCC
NAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
AAAGANATVQTNNVKAGELACDYIAKKLNGKGNVIIENGPQVSAVIDRVNGCKAVLAKSP
EECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEECCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDAVFTINDPQAIGSDLAAKQLNRSGIVI
CCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEE
TSVDGAPDIEVALKSNTLVQASASQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLVT
EECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEE
RDNVKSYKGWSTPR
CCCCHHCCCCCCCC
>Mature Secondary Structure
MFKHKAALTALACAFAFGASAAHAADKPLKLIGVTVGSLGNPYFVTIVKSAEARAKQINP
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCHHHHHCCC
NAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
AAAGANATVQTNNVKAGELACDYIAKKLNGKGNVIIENGPQVSAVIDRVNGCKAVLAKSP
EECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEECCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDAVFTINDPQAIGSDLAAKQLNRSGIVI
CCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEE
TSVDGAPDIEVALKSNTLVQASASQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLVT
EECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEE
RDNVKSYKGWSTPR
CCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]