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The map label for this gene is citH [H]

Identifier: 161524821

GI number: 161524821

Start: 1824098

End: 1825384

Strand: Direct

Name: citH [H]

Synonym: Bmul_1648

Alternate gene names: 161524821

Gene position: 1824098-1825384 (Clockwise)

Preceding gene: 161524820

Following gene: 161524824

Centisome position: 52.9

GC content: 66.9

Gene sequence:

>1287_bases
ATGAATACGACCACGCTGACCACGCAGGACGCCGCACGCCCCAGCGCGGCGCAGATCCGGCGCATCATTTTCGCGGCGTC
GATCGGCAACGCGCTCGAATGGTTCGACCTGATCGTCTACGGCTTTTTCGCGGTGACGATCGCGAAGCTGTTCTTCCCCG
CGACGAGCGAAGCGACGTCGCTGCTGCTCACGCTCGGCACGTTCGGGCTGTCCTATCTGATCCGCCCAATCGGTGGCTTC
GTGCTCGGCGCGTATGCGGATCGCGCGGGCCGCAAGGCGTCGCTGCTGCTGTCGATCGCGATGATGATGGGCGGCACACT
GCTGATCGCCGCGATGCCGACCTACGCGTCGATCGGCATTCTCGCGCCGCTCGGGATCATGCTGTCGCGGCTGATGCAGG
GCTTTTCCGCCGGCGGCGAGTTCGCGAGTTCGACCGCGTTTCTCGTCGAGCATGCGCCGCAGCGGCGCGGCTTCATGTCG
AGCTGGCAATTCGCGAGCCAGGGCCTCGCGACGCTGCTCGCCTCGGGGTTCGGCGCCCTGCTGACGTCCACGCTGACGCC
CGCGCAACTCGAAAGCTGGGGCTGGCGCGTGCCGTTCCTGTTCGGCCTCGCCATTGGCCCGGTCGGACTCTACATCCGCC
GCTACGTCGACGAAGGCGTCGAGTTCCGCACGCAGGCGCGTGCCGAAGCGCCGGTGCGCGAACTCTTCACGCACTACAAG
ATGCGGTTGCTGCTCGCGATCGGCGCGCTCGTGATCTCGACCGCGATCAACTACATGGTGCTGTACATGCCGACGTACGC
GATCAAGCAGCTCGGACTGCCGGCGTCGACCGGATTCGCGGCGACGCTCGCGACCGGCTTCGTGCTGACGCTCGTCACGC
CGCTCGTCGGGCACCTGTCCGATCGCACCGGACGGATCCGCGTGATGACGACCGCGGCGCTGCTGATGCTGCTGACCGTC
TGGCCGACGTTCGCGTGGCTCACGCGGCACGCGTCGTTCGCGACGATGCTCGCCGCGCTGATCTGGATCGGCGCGCTGAA
GGCGATGTATTGCGGTGCGCTGCCCGCGTTGATGTCGGAACTGTTTCCCGCGCAGACGCGCGCGACGGGCCTTGCCGTCA
GCTACAACACGGGCGTCACGCTGTTCGGCGGCTTCGCGCCGTTCGTCATCACCTGGCTGATCAGCGCGACTGGCAACCGG
CTGTCGCCTGCGCTGTATCTGATCGGATGCGCGATCCTCAGTCTCGTCGCGCTGTTCGTCGCACGTACGCGGCTGCGGAT
GCAATAG

Upstream 100 bases:

>100_bases
ACGCGCCGCATCCGCGCCGCACGACGTCGGCATCGGCGTCTGCCGCGGCCGGCATCGCTGCGTAACGCGTGCTGCATCCT
TCCACGGAGCCCCATCCACG

Downstream 100 bases:

>100_bases
CGGAAACGGCAGCGCGATGCGCTGCCGATGGAAAACGGCCCGCGCCGGCATCGCGCCGCGCGGGCCGCACGTCGTTTACG
GCGCCGGGTTCGGCTGCTGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTTTLTTQDAARPSAAQIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLLLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMGGTLLIAAMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTSTLTPAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFRTQARAEAPVRELFTHYK
MRLLLAIGALVISTAINYMVLYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPLVGHLSDRTGRIRVMTTAALLMLLTV
WPTFAWLTRHASFATMLAALIWIGALKAMYCGALPALMSELFPAQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLIGCAILSLVALFVARTRLRMQ

Sequences:

>Translated_428_residues
MNTTTLTTQDAARPSAAQIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLLLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMGGTLLIAAMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTSTLTPAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFRTQARAEAPVRELFTHYK
MRLLLAIGALVISTAINYMVLYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPLVGHLSDRTGRIRVMTTAALLMLLTV
WPTFAWLTRHASFATMLAALIWIGALKAMYCGALPALMSELFPAQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLIGCAILSLVALFVARTRLRMQ
>Mature_428_residues
MNTTTLTTQDAARPSAAQIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLLLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMGGTLLIAAMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTSTLTPAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFRTQARAEAPVRELFTHYK
MRLLLAIGALVISTAINYMVLYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPLVGHLSDRTGRIRVMTTAALLMLLTV
WPTFAWLTRHASFATMLAALIWIGALKAMYCGALPALMSELFPAQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLIGCAILSLVALFVARTRLRMQ

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=32.0843091334895, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788942, Length=390, Percent_Identity=36.1538461538462, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=31.4220183486239, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=32.3255813953488, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87082231, Length=448, Percent_Identity=23.8839285714286, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=413, Percent_Identity=24.2130750605327, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46007; Mature: 46007

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTTLTTQDAARPSAAQIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMGGTLLIAAMPTYASIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTSTLTPAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFRTQARAEAPVRELFTHYK
HHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHH
MRLLLAIGALVISTAINYMVLYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPLVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DRTGRIRVMTTAALLMLLTVWPTFAWLTRHASFATMLAALIWIGALKAMYCGALPALMSE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPAQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNRLSPALYLIGCAILSLVALFV
HCCCHHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLRMQ
HHHHHCCC
>Mature Secondary Structure
MNTTTLTTQDAARPSAAQIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMGGTLLIAAMPTYASIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTSTLTPAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFRTQARAEAPVRELFTHYK
HHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHH
MRLLLAIGALVISTAINYMVLYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPLVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DRTGRIRVMTTAALLMLLTVWPTFAWLTRHASFATMLAALIWIGALKAMYCGALPALMSE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPAQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNRLSPALYLIGCAILSLVALFV
HCCCHHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLRMQ
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]