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The map label for this gene is ogt [H]

Identifier: 161523916

GI number: 161523916

Start: 809029

End: 809499

Strand: Direct

Name: ogt [H]

Synonym: Bmul_0736

Alternate gene names: 161523916

Gene position: 809029-809499 (Clockwise)

Preceding gene: 161523915

Following gene: 161523917

Centisome position: 23.46

GC content: 68.15

Gene sequence:

>471_bases
ATGTTCAACGCAGTAATCGACGCGCCGTTCGGCAAGGTCGGCATCCGCACGGATGCGGCCGCGGTGCGCGAAATCGTCTA
TCTGCCCGAGTCGGTGAAGTCCGTCGCGCCGGACACGCCGCTCGCGAAGCGCGCGGTCGAGCAGATCGAGCGGTATTTCG
AGCGCGCGTCGGCCCGTTTCGACCTGCCGCTCGCGCCGGTCGGCAGCGCGTTCCAGCACCGCGTGTGGGACGTGATCAGC
GACATTCCGCCCGGCACCGTGCTGACGTACGGGCAGGTCGCGAAGCGGATCGGCAGCGCGCCGCGCGCGGTCGGCCAGGC
ATGCGGTGCGAACTATTTTCCGCTGGTGATCCCGTGTCACCGCGTCGTCGCCGCGAGCGGGCTCGGCGGTTTCGCGAATC
ACGACGACGACGGCTATTACCTGAAAGTGAAGCGCTGGCTGCTGGCGCACGAGGGCGTGCCGTACCGATGA

Upstream 100 bases:

>100_bases
GGCGTAAAGTGTCGTCGGCGCGCGAGCGCGACGCGCGCGCGACGGTCTCGACCGGCGGGATGCGCGTCGCATCGGCGCCG
GTGTCGGTGAGGAGAATGCC

Downstream 100 bases:

>100_bases
GCGAACCGCTGCTGTCTCCCGAAGCCGGCGACGGTACGGCCGCGCCGTCGCCCGCGCTGCTCGCGAGCCGCGCGTCGATC
GACCTGTTCTGCGATGCGCT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFNAVIDAPFGKVGIRTDAAAVREIVYLPESVKSVAPDTPLAKRAVEQIERYFERASARFDLPLAPVGSAFQHRVWDVIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAASGLGGFANHDDDGYYLKVKRWLLAHEGVPYR

Sequences:

>Translated_156_residues
MFNAVIDAPFGKVGIRTDAAAVREIVYLPESVKSVAPDTPLAKRAVEQIERYFERASARFDLPLAPVGSAFQHRVWDVIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAASGLGGFANHDDDGYYLKVKRWLLAHEGVPYR
>Mature_156_residues
MFNAVIDAPFGKVGIRTDAAAVREIVYLPESVKSVAPDTPLAKRAVEQIERYFERASARFDLPLAPVGSAFQHRVWDVIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAASGLGGFANHDDDGYYLKVKRWLLAHEGVPYR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=31.3953488372093, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1787596, Length=133, Percent_Identity=36.0902255639098, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1788542, Length=117, Percent_Identity=35.8974358974359, Blast_Score=70, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=41.3793103448276, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16993; Mature: 16993

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAVIDAPFGKVGIRTDAAAVREIVYLPESVKSVAPDTPLAKRAVEQIERYFERASARF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DLPLAPVGSAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCH
CCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVAASGLGGFANHDDDGYYLKVKRWLLAHEGVPYR
HHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFNAVIDAPFGKVGIRTDAAAVREIVYLPESVKSVAPDTPLAKRAVEQIERYFERASARF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DLPLAPVGSAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCH
CCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVAASGLGGFANHDDDGYYLKVKRWLLAHEGVPYR
HHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]