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The map label for this gene is glyS [H]

Identifier: 161523777

GI number: 161523777

Start: 651955

End: 654054

Strand: Reverse

Name: glyS [H]

Synonym: Bmul_0597

Alternate gene names: 161523777

Gene position: 654054-651955 (Counterclockwise)

Preceding gene: 161523778

Following gene: 161523776

Centisome position: 18.97

GC content: 69.1

Gene sequence:

>2100_bases
ATGACGCACAATCATCCCGCCCCCCTCCTCGTCGAGCTGCTGACCGAAGAGCTGCCGCCGAAGGCCCTCGCGCGCCTCGG
CGACGCATTTGCCGAAGGCCTCGCGCAACGCCTCGCCGCGCGCGATCTCGTCGAAGGCGAGCTCGTGTTCGAACGCTATG
CGACGCCGCGCCGCCTCGCGGTCGTCGTGCAGAACGTGCGCGCGGTCGCACCGGACCGCCAGGTCCGCGAAAAGGTGCTG
CCGGTGTCGGTCGCACTCGACGCGCAAGGCAATCCGACCGCCCCGCTCGCGAAGAAACTCGCGGCGCTCGGCCGTCCGAA
CCTGACGATCGCCGAGCTCGAGCGCGCACAGGACGGCAAGGCCGAAGCGTTCTTCATCAACTATTCGGCCGCCGGCGCGA
CGCTCGCCGACGGGCTGCAGGCCGCGCTCGACGAGACGCTCGCGAAGCTGCCGATCCCGAAGGTCATGACCTATCAGCGC
CCGGACGGCAGCGACGTGCAGTTCGTGCGTCCCGTGCATCGGCTGACGGTGCTGCACGACGATCGCGTCGTGCCCGTCAC
CGCGTTCGGCATCGACGCCGGCGACACGACGCTCGGCCATCGCTTCCTGTCCGACGGGCTCGTCGCGATCCAGCATGCGA
GCGCGTACGCCGAGACGCTGCGCGACAAGGGCCGCGTGATCGCGCATTTCGGCGATCGCAAGGAAACGATCCGCACGCAG
CTGAACGAGCATGCGAACGGCGACACGGTCGTGATGCCCGATGCGCTGCTCGACGAAGTGACGTCGCTCGTCGAATGGCC
GGTCGTCTATCCGTGCCGCTTCGAGGACGAATTCCTGCAGGTGCCGCAGGAATGCCTGATCCTCACGATGCAGACGAACC
AGAAGTATTTCGCGCTGACCGACATCGGCGGCAAGCTGCGCTCGCGCTTCCTGATCGTGTCGAACATCGAGACGAAGACG
CCGGGCGAAATCGTCGAGGGCAACGAGCGCGTCGTGCGTCCGCGCCTCGCCGATGCGAAGTTCTTCTTCGAGCAGGACAA
GAAGAAGCCGCTCGCCGCGCGCGTGCCGCTGCTCGCGAACGTCGTCTATCACAACAAGCTCGGCTCGGCGCTGGCGCGCG
TCGAGCGCCTCGAAGCGCTGGCCGGCGAGATCGCGCCCGCGATCGGCGCCGACGTCGCGCATGCGAAGCGCGCCGCGCGC
CTCGCGAAGGCCGACCTGCTGACCGACATGGTCGGCGAGTTCCCGGAACTGCAGGGCACGATGGGCACGTACTACGCGCG
CCACGACGGCGAGCCCGACGACGTCGCGCTCGCGTGCGCCGAGCACTACCAGCCGCGCTTCTCGGGCGACACGCTGCCGA
CCACGCCGGTGTCGACGGCCGTCGCGCTCGCGGACAAGCTCGAAACGATCGTCGGCATCTGGGGCATCGGCCTCGCGCCG
ACCGGCGAGAAGGATCCGTTCGCGCTGCGCCGCCACGCGCTCGGCGTGCTGCGCCTGCTGATCGAGAAGCAGCTGCCGCT
CGACCTCGTGTCGCTGCTGCGTACCGCCTACGCGCGCTTCGAAGGCGTGCCGGGCGTTGCGGAATCGACCGACGCGATCG
TCGCGTTCTTCATGGACCGCCTGCGCGGCCTGCTGCGCGAGCGCGGCTACTCGGCCGGCGAAGTCGACGCGGTGCTGAGC
CTGAACCCGACGCGCCTCGACGATCTCGTCGCGCGCCTCGACGCGGTGCGCGAATTCACGCGTCTCGCGGAAGCCGAAGC
GCTCGCGGCCGCGAACAAGCGGATCTCGAACATCCTGAAGAAATCGGAAGGCGGCGCGAACGGCGCCGTGCAGCCGACGC
TGCTCGTCGAGGCGGCCGAGAAGGCGCTGCACGAACAGCTCGCGGCCGTGACGCCGCACGTGCAGTCGCAGCTCGAGGCA
CGCGCGTACACGGGCGCGCTGTCGGCGCTCGCCGCGCTGCGTGCGCCGGTCGATACGTTCTTCAACGACGTGATGGTCAA
CGCCGACGATCCGGCGCTGCGCGCGAACCGTCTGGCGCTGCTGTCCGCCCTGCATCAGCAGATGAACTGCGTCGCCGACA
TCTCGAAGCTCGCCGCATAA

Upstream 100 bases:

>100_bases
ACCACCGATGCCCAGGATGCCGCGCAGCCGGCGTGGGCGCCGCCGCTGAAGGTCGAACGCAAGATCGATCAGGACTGACG
AGACGAGATTTCTTCACACC

Downstream 100 bases:

>100_bases
GGGCCGCACGATGCCGACCAGCCCCAACCGCAAGCTCGTCGTCCTCGATCGCGACGGCGTGATCAACGTCGATTCGGACG
CGTTCATCAAGACGCCCGAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGLAQRLAARDLVEGELVFERYATPRRLAVVVQNVRAVAPDRQVREKVL
PVSVALDAQGNPTAPLAKKLAALGRPNLTIAELERAQDGKAEAFFINYSAAGATLADGLQAALDETLAKLPIPKVMTYQR
PDGSDVQFVRPVHRLTVLHDDRVVPVTAFGIDAGDTTLGHRFLSDGLVAIQHASAYAETLRDKGRVIAHFGDRKETIRTQ
LNEHANGDTVVMPDALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDIGGKLRSRFLIVSNIETKT
PGEIVEGNERVVRPRLADAKFFFEQDKKKPLAARVPLLANVVYHNKLGSALARVERLEALAGEIAPAIGADVAHAKRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACAEHYQPRFSGDTLPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLIEKQLPLDLVSLLRTAYARFEGVPGVAESTDAIVAFFMDRLRGLLRERGYSAGEVDAVLS
LNPTRLDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEA
RAYTGALSALAALRAPVDTFFNDVMVNADDPALRANRLALLSALHQQMNCVADISKLAA

Sequences:

>Translated_699_residues
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGLAQRLAARDLVEGELVFERYATPRRLAVVVQNVRAVAPDRQVREKVL
PVSVALDAQGNPTAPLAKKLAALGRPNLTIAELERAQDGKAEAFFINYSAAGATLADGLQAALDETLAKLPIPKVMTYQR
PDGSDVQFVRPVHRLTVLHDDRVVPVTAFGIDAGDTTLGHRFLSDGLVAIQHASAYAETLRDKGRVIAHFGDRKETIRTQ
LNEHANGDTVVMPDALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDIGGKLRSRFLIVSNIETKT
PGEIVEGNERVVRPRLADAKFFFEQDKKKPLAARVPLLANVVYHNKLGSALARVERLEALAGEIAPAIGADVAHAKRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACAEHYQPRFSGDTLPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLIEKQLPLDLVSLLRTAYARFEGVPGVAESTDAIVAFFMDRLRGLLRERGYSAGEVDAVLS
LNPTRLDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEA
RAYTGALSALAALRAPVDTFFNDVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
>Mature_698_residues
THNHPAPLLVELLTEELPPKALARLGDAFAEGLAQRLAARDLVEGELVFERYATPRRLAVVVQNVRAVAPDRQVREKVLP
VSVALDAQGNPTAPLAKKLAALGRPNLTIAELERAQDGKAEAFFINYSAAGATLADGLQAALDETLAKLPIPKVMTYQRP
DGSDVQFVRPVHRLTVLHDDRVVPVTAFGIDAGDTTLGHRFLSDGLVAIQHASAYAETLRDKGRVIAHFGDRKETIRTQL
NEHANGDTVVMPDALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDIGGKLRSRFLIVSNIETKTP
GEIVEGNERVVRPRLADAKFFFEQDKKKPLAARVPLLANVVYHNKLGSALARVERLEALAGEIAPAIGADVAHAKRAARL
AKADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACAEHYQPRFSGDTLPTTPVSTAVALADKLETIVGIWGIGLAPT
GEKDPFALRRHALGVLRLLIEKQLPLDLVSLLRTAYARFEGVPGVAESTDAIVAFFMDRLRGLLRERGYSAGEVDAVLSL
NPTRLDDLVARLDAVREFTRLAEAEALAAANKRISNILKKSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEAR
AYTGALSALAALRAPVDTFFNDVMVNADDPALRANRLALLSALHQQMNCVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=44.5730824891462, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75847; Mature: 75716

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHNHPAPLLVELLTEELPPKALARLGDAFAEGLAQRLAARDLVEGELVFERYATPRRLA
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPDRQVREKVLPVSVALDAQGNPTAPLAKKLAALGRPNLTIAELERAQDGK
HHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCC
AEAFFINYSAAGATLADGLQAALDETLAKLPIPKVMTYQRPDGSDVQFVRPVHRLTVLHD
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHHHHHHEEEC
DRVVPVTAFGIDAGDTTLGHRFLSDGLVAIQHASAYAETLRDKGRVIAHFGDRKETIRTQ
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
LNEHANGDTVVMPDALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEEECHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCCEEEEE
DIGGKLRSRFLIVSNIETKTPGEIVEGNERVVRPRLADAKFFFEQDKKKPLAARVPLLAN
CCCHHHHHCEEEEECCCCCCCCCHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSALARVERLEALAGEIAPAIGADVAHAKRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEPDDVALACAEHYQPRFSGDTLPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLIEKQLPLDLVSLLRTAYARFEGVPGVAESTDAIVAFFMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRGLLRERGYSAGEVDAVLSLNPTRLDDLVARLDAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEARAYTGALSALAALRAPVDTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNDVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
HHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THNHPAPLLVELLTEELPPKALARLGDAFAEGLAQRLAARDLVEGELVFERYATPRRLA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPDRQVREKVLPVSVALDAQGNPTAPLAKKLAALGRPNLTIAELERAQDGK
HHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCC
AEAFFINYSAAGATLADGLQAALDETLAKLPIPKVMTYQRPDGSDVQFVRPVHRLTVLHD
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHHHHHHEEEC
DRVVPVTAFGIDAGDTTLGHRFLSDGLVAIQHASAYAETLRDKGRVIAHFGDRKETIRTQ
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
LNEHANGDTVVMPDALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEEECHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCCEEEEE
DIGGKLRSRFLIVSNIETKTPGEIVEGNERVVRPRLADAKFFFEQDKKKPLAARVPLLAN
CCCHHHHHCEEEEECCCCCCCCCHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSALARVERLEALAGEIAPAIGADVAHAKRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEPDDVALACAEHYQPRFSGDTLPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLIEKQLPLDLVSLLRTAYARFEGVPGVAESTDAIVAFFMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRGLLRERGYSAGEVDAVLSLNPTRLDDLVARLDAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGGANGAVQPTLLVEAAEKALHEQLAAVTPHVQSQLEARAYTGALSALAALRAPVDTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNDVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
HHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA