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The map label for this gene is metH [H]

Identifier: 161523568

GI number: 161523568

Start: 429232

End: 430335

Strand: Reverse

Name: metH [H]

Synonym: Bmul_0388

Alternate gene names: 161523568

Gene position: 430335-429232 (Counterclockwise)

Preceding gene: 161523569

Following gene: 161523567

Centisome position: 12.48

GC content: 68.21

Gene sequence:

>1104_bases
ATGTCGGTTTCCCGTCTTCGCCGCTCTTTCGCCATGTCCGCGACACCTACCGCCGCTTCCGCCGCCCCGCTCGACGCGTC
CTACACGCGCGGCGCCGAACTGCCCGCGCTGCTGAAATCGCGGATCCTGATCCTCGACGGCGCGATGGGCACGATGATCC
AGCGCTACAAGCTCGACGAAGCCGCGTATCGCGGCGAGCGCTTCAAGGATTTCCCGCGCGACGTGAAGGGCAACAACGAG
CTGCTGTCGATCACGCAGCCGCAGGTGATCCGCGAGATCCACGACCAGTACTTCGCGGCGGGCGCCGACATCGTCGAAAC
CAATACGTTCGGCGCGACGACGGTCGCGCAGGCCGACTACGGGATGGAAGACCTCGTCGTCGAGATGAACGTCGAATCGG
CAAAGCTCGCGCGCGCGTCGGCCGCGCAGTACGCGACGCCGGACAAGCCGCGCTTCGTCGCCGGCGCGATCGGCCCGACG
CCGAAGACGGCGAGCATCTCGCCCGACGTCAACGATCCGGGCGCGCGCAACGTGACGTTCGACGAGCTGCGCGACGCGTA
CTACATGCAGGCGAAGGCGCTGCTCGACGGCGGCGTCGATCTGTTCCTCGTCGAGACGATCTTCGACACGCTGAACGCGA
AGGCCGCGCTGTTCGCGCTCGACCAGCTGTTCGAGGACACCGGCGAGCGTCTGCCGATCATGATCTCGGGCACCGTGACC
GACGCGTCGGGCCGCATCCTGTCGGGCCAGACGGTCGAGGCGTTCTGGAATTCGCTGCGCCACGCGAAGCCGCTGACGTT
CGGTCTGAACTGCGCACTCGGCGCGGCGCTGATGCGCCCGTACATCGCCGAGCTCGCGAAGCTCTGCGACACGTTCGTCT
CGTGCTATCCGAACGCGGGCCTGCCGAACCCGATGGCCGAAACCGGCTTCGACGAGACGCCCGACGTCACGTCGGGGCTG
CTGCGCGAGTTCGCGCAGGCCGGCCTCGTGAACCTCGCCGGCGGCTGCTGCGGCACGACGCCCGAGCACATCGCCGAGAT
CGCGAAGGCGCTCGCCGACGTGAAGCCGCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CCACGCCACGCACCACGCACCGTACGCCCAGCGCCCACGCACCGCGGGCCGGGCCGCGCAAGCCCGCGCGGCCGCGCGGG
CGCGCGGCGCACCGGCTACA

Downstream 100 bases:

>100_bases
TCCGACTGCCCGAAGCACGCCCCTCATTACACGAATTCGCACCGCCATGACCGATCACACGATGCGCCTTGCCGGCCTCG
AGCCGTTCAACGTCACGTCC

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSVSRLRRSFAMSATPTAASAAPLDASYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNE
LLSITQPQVIREIHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESAKLARASAAQYATPDKPRFVAGAIGPT
PKTASISPDVNDPGARNVTFDELRDAYYMQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVT
DASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTFVSCYPNAGLPNPMAETGFDETPDVTSGL
LREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSQYSEAA

Sequences:

>Translated_367_residues
MSVSRLRRSFAMSATPTAASAAPLDASYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNE
LLSITQPQVIREIHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESAKLARASAAQYATPDKPRFVAGAIGPT
PKTASISPDVNDPGARNVTFDELRDAYYMQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVT
DASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTFVSCYPNAGLPNPMAETGFDETPDVTSGL
LREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSQYSEAA
>Mature_366_residues
SVSRLRRSFAMSATPTAASAAPLDASYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNEL
LSITQPQVIREIHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESAKLARASAAQYATPDKPRFVAGAIGPTP
KTASISPDVNDPGARNVTFDELRDAYYMQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTD
ASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTFVSCYPNAGLPNPMAETGFDETPDVTSGLL
REFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSQYSEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=330, Percent_Identity=58.1818181818182, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1790450, Length=331, Percent_Identity=59.5166163141994, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786456, Length=326, Percent_Identity=26.6871165644172, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=331, Percent_Identity=55.2870090634441, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 39477; Mature: 39346

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSRLRRSFAMSATPTAASAAPLDASYTRGAELPALLKSRILILDGAMGTMIQRYKLDE
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHH
AAYRGERFKDFPRDVKGNNELLSITQPQVIREIHDQYFAAGADIVETNTFGATTVAQADY
HHHCCCHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCC
GMEDLVVEMNVESAKLARASAAQYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTF
CHHHEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCH
DELRDAYYMQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEE
DASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTFVSCYPNAG
CCCCCEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPNPMAETGFDETPDVTSGLLREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWP
CCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
SQYSEAA
CHHCCCC
>Mature Secondary Structure 
SVSRLRRSFAMSATPTAASAAPLDASYTRGAELPALLKSRILILDGAMGTMIQRYKLDE
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHH
AAYRGERFKDFPRDVKGNNELLSITQPQVIREIHDQYFAAGADIVETNTFGATTVAQADY
HHHCCCHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCC
GMEDLVVEMNVESAKLARASAAQYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTF
CHHHEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCH
DELRDAYYMQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEE
DASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTFVSCYPNAG
CCCCCEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPNPMAETGFDETPDVTSGLLREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWP
CCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
SQYSEAA
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]