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The map label for this gene is metH [H]

Identifier: 161523567

GI number: 161523567

Start: 426468

End: 429185

Strand: Reverse

Name: metH [H]

Synonym: Bmul_0387

Alternate gene names: 161523567

Gene position: 429185-426468 (Counterclockwise)

Preceding gene: 161523568

Following gene: 161523565

Centisome position: 12.45

GC content: 65.12

Gene sequence:

>2718_bases
ATGACCGATCACACGATGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGAACGCTCTTCATCAACGTCGGCGA
ACGCACCAACGTCACCGGCTCGAAGGCGTTCGCCCGGATGATCCTCAACGGCCAGTTCGACGAGGCGCTCGCGGTCGCGC
GCCAGCAGGTCGAAAACGGCGCGCAGGTGATCGACGTCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCGATGGTG
CGCTTCCTGAATCTGATCGCGTCGGAGCCTGACATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGACGTGAT
CGAGGCCGGTCTCAAGTGCGTGCAGGGCAAGGCGATCGTGAACTCGATCTCGCTGAAGGAAGGCGAGGACGCGTTCGTCC
ATCACGCGAAGCTGATCCGCCGCTACGGCGCGGCGGCCGTCGTGATGGCATTCGACGAGCAGGGCCAGGCCGACACGTTC
GCGCGCAAGACCGAAATCTGCCGGCGTTCGTACGACGTGCTCGTGAACCGCTGCGGCTTTCCGCCCGAGGACATCATCTT
CGATCCGAACATCTTCGCGGTCGCGACCGGCATCGAGGAGCACAACAACTACGCGGTCGACTTCATCGAAGCGACGCGCT
GGATCAAGCAGAACCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTCCGCGGCAACGATCCG
GTGCGCGAGGCGATCCACACGGTGTTCCTCTACCACGCGATCCAGGCCGGGATGGACATGGGGATCGTCAACGCGGGCCA
GCTCGGCGTGTATGCGGATCTCGATCCGGAGCTGCGCGAGCGCGTCGAGGACGTGATCCTGAACCGCCGCCCCGACTCCA
CCGACCGGCTGCTCGAGATCGCCGACAAGTTCAAGACCGGCGCCGCGAAGAAGGAAGAGAACCTCGAGTGGCGCAATCAG
CCGGTCGAGAAGCGGCTCGCGCACGCGCTCGTGCACGGCATCACGAACTTCATCGTCGAAGATACCGAGGAAGCGCGCGT
GAAGATCGCCGCGGCCGGCGGCCGCCCGATCAACGTGATCGAAGGGCCGCTGATGGACGGCATGAACATCGTCGGCGACC
TGTTCGGCCAGGGCAAGATGTTCCTGCCGCAGGTCGTGAAGTCCGCGCGCGTGATGAAGCAGGCGGTCGCTCACCTGATC
CCGTTCATCGAGGAAGAAAAGCGGCTGCTCGCGGAAGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTGATCGCGAC
CGTCAAGGGCGACGTCCACGACATCGGCAAGAACATCGTGTCGGTCGTGCTCCAGTGCAACAACTTCGAAGTCGTCAACA
TGGGCGTGATGGTGCCGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAAGGCGCGGACATCATCGGGCTGTCCGGCCTG
ATCACGCCGAGCCTCGAGGAAATGGCCTACGTCGCGTCCGAAATGCAGCGCGACGACTATTTCCGTGTGAAGAAGATTCC
GCTGCTGATCGGCGGCGCGACGACGTCGCGCGTGCACACGGCCGTGAAGATCGCGCCGAACTACGAAGGCCCGGTCGTCT
ACGTGCCCGATGCGTCGCGCTCGGTGTCGGTCGCATCGAGCCTGCTGTCCGACGAAGGCGCGACGAAGTACCTCGACGAG
CTGAAGGCCGACTACGAGCGGATCCGCGATCAGCACGCGAACCGCAAGGCGCAGCCGATGGTGACGCTCGCCGAGGCGCG
CGCGAACAAGACGAAGATCGACTGGGCCAACTACACGCCGGTGAAGCCGAAGTTCATCGGCCGCCGCGTATTCCGGAACT
ACGACCTGAACGAACTCGCGAACTTCATCGACTGGGGTCCGTTCTTCCAGACCTGGGACCTCGCGGGCCCGTACCCGGCG
ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGCCGCGTGTTCTCCGACGCGAAGTCGATGCTCGCGCGGCTGATCCA
GGGCCGCTGGCTGACCGCGAACGGCGTGATCGCGCTGCTGCCGGCGAATAGCGTCAACGACGACGACATCGAAATCTACA
CCGACGAATCGCGCACCGACGTGCTGCTCACGTGGCGCAACCTGCGTCAGCAGAGCATGCGTCCCGTCGTCGACGGCGTG
ATGCGGCCGAACCGCTCGCTCGCCGACTTCATCGCGCCGAAGGAATCGGGCGTCGCCGACTACATCGGGATGTTCGCGGT
GACGGCCGGCCTCGGCGTCGATGTGAAGGAAAAGCAGTTCGAAGCCGATCACGACGACTACAGCGCGATCATGCTGAAGG
CGCTCGCGGACCGCTTCGCGGAAGCGTTCGCCGAAGCGATGCACGCGCGCGTGCGCCGCGAGCTGTGGGGCTATGCGAGC
GACGAGACGCTCGACAACGACGCGCTGATCGCCGAAAAGTACACGGGCATCCGCCCGGCGCCCGGCTATCCGGCATGCCC
GGACCACCTCGTCAAACGCGACATGTTCGCCGCGCTGCACGCGGACGAGATCGGCATGAGCGTCACCGATTCGCTGGCGA
TGCTGCCGGCCGCGAGCGTCTCGGGTTTCTATCTCGCGCATCCGGACAGCCGCTACTTCTCGGTCGGGAAAATCGGTCAG
GATCAGCTCGAGGACTACGCGCGCCGGATGGCGCTGTCGCTCGACGACGCGCGGCGCGCGCTCGCGCCGCAGCTCTGA

Upstream 100 bases:

>100_bases
CTCGCCGACGTGAAGCCGCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGATCCGACTGCCCGAAGCACGCCCCTCA
TTACACGAATTCGCACCGCC

Downstream 100 bases:

>100_bases
GCGCGGCCGCGCGCCGCGGCCGTTTCGTCAGACAAAAAAAGCCCGCGCGAACGCGGGCTTTTTGCTTCGTTGCGGCTCGT
GCTGGCGGCGGTCGCCGTGG

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEDAFVHHAKLIRRYGAAAVVMAFDEQGQADTF
ARKTEICRRSYDVLVNRCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRPDSTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLAHALVHGITNFIVEDTEEARVKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATKYLDE
LKADYERIRDQHANRKAQPMVTLAEARANKTKIDWANYTPVKPKFIGRRVFRNYDLNELANFIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANSVNDDDIEIYTDESRTDVLLTWRNLRQQSMRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
DETLDNDALIAEKYTGIRPAPGYPACPDHLVKRDMFAALHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLEDYARRMALSLDDARRALAPQL

Sequences:

>Translated_905_residues
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEDAFVHHAKLIRRYGAAAVVMAFDEQGQADTF
ARKTEICRRSYDVLVNRCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRPDSTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLAHALVHGITNFIVEDTEEARVKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATKYLDE
LKADYERIRDQHANRKAQPMVTLAEARANKTKIDWANYTPVKPKFIGRRVFRNYDLNELANFIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANSVNDDDIEIYTDESRTDVLLTWRNLRQQSMRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
DETLDNDALIAEKYTGIRPAPGYPACPDHLVKRDMFAALHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLEDYARRMALSLDDARRALAPQL
>Mature_904_residues
TDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEDAFVHHAKLIRRYGAAAVVMAFDEQGQADTFA
RKTEICRRSYDVLVNRCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDPV
REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRPDSTDRLLEIADKFKTGAAKKEENLEWRNQP
VEKRLAHALVHGITNFIVEDTEEARVKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATKYLDEL
KADYERIRDQHANRKAQPMVTLAEARANKTKIDWANYTPVKPKFIGRRVFRNYDLNELANFIDWGPFFQTWDLAGPYPAI
LNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANSVNDDDIEIYTDESRTDVLLTWRNLRQQSMRPVVDGVM
RPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASD
ETLDNDALIAEKYTGIRPAPGYPACPDHLVKRDMFAALHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQD
QLEDYARRMALSLDDARRALAPQL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=915, Percent_Identity=54.7540983606557, Blast_Score=977, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.7212389380531, Blast_Score=1111, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.7149122807018, Blast_Score=947, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 100001; Mature: 99870

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDVNMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEDAFVHHAKLIRRYGAAAVVMAFDEQGQADTFARKTEICRRSYDVLVNRCGF
HHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRPDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLAHALVHGITNFIVEDTEEARVKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGATKYLDELKADYERIRDQHANRKAQPMVTLAEARANKTKIDWANYTP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCC
VKPKFIGRRVFRNYDLNELANFIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANSVNDDDIEIYTDESRTDVLLTWRNLRQQSMRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASDETLDNDALIAEKYTGIRPAPGYPACPDHLVKRDMFAALH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLEDYARRMALSLDDARRA
HHHCCCHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCHHHHHHH
LAPQL
HCCCC
>Mature Secondary Structure 
TDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDVNMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEDAFVHHAKLIRRYGAAAVVMAFDEQGQADTFARKTEICRRSYDVLVNRCGF
HHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRPDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLAHALVHGITNFIVEDTEEARVKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGATKYLDELKADYERIRDQHANRKAQPMVTLAEARANKTKIDWANYTP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCC
VKPKFIGRRVFRNYDLNELANFIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANSVNDDDIEIYTDESRTDVLLTWRNLRQQSMRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASDETLDNDALIAEKYTGIRPAPGYPACPDHLVKRDMFAALH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLEDYARRMALSLDDARRA
HHHCCCHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCHHHHHHH
LAPQL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]