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The map label for this gene is kipI [H]

Identifier: 161523546

GI number: 161523546

Start: 403207

End: 404082

Strand: Reverse

Name: kipI [H]

Synonym: Bmul_0366

Alternate gene names: 161523546

Gene position: 404082-403207 (Counterclockwise)

Preceding gene: 161523547

Following gene: 161523545

Centisome position: 11.72

GC content: 66.32

Gene sequence:

>876_bases
GTGACGACCCGCTATACGTTCGGCGGCGACGAGTTCGTGTTCGTCGAGATCAGCGAAGCGATGTCGCTCGACGCCTTCTT
CAAGGGCACCGCGATCACGCGCGAGCTGCAGCGCCGCGCGGTGCCCGGCATCACCGAGATCTGCCCGGCCAACGCGTCCT
ACCAGGTGCGCTACGACCCGGACACGATCGCGCCCGACGCGCTCGTCGCGCTGCTGAAGTCGATCGAAGCCGACGTCGGC
GAAGCGTCGCTCGACCTCGACACGCGCATCGTCGAGGTGCCGGTGCTCTACAACGATCCGTGGACGCACGAGACGATGAT
GCGGTTCCGCGAGCGTCATCAGGATCCGTCGTCGACCGATCTCGAATACGCGGCGCGCATCAACGGCAAGCGCGACGTCG
ACGACTTCATCGCCGCGCACTCGGGCGCGCCGTGGTTCGTGTCGATGGTCGGCTTCGTCGCGGGCCTGCCGTTCATGTAC
CAGATGGTCGAACGCGAACGCCAGCTCGAAGTACCGAAATACCTGCGGCCGCGCACCGACACGCCGAAGCTGACGGTCGG
CCATGGCGGCTGCTTCGGCTGCATCTACTCGGTGCGCGGCGCGGGCGGCTACCAGATGTTCGGCGTGACGCCGGCGCCGA
TCTTCGATCCCGAACAGCGGCTCGACTATCTGCGCGACTTCATGGTGTTCTTCCGCCCCGGCGACATCGTGAAGTTCCAG
CCGATCGATCGCGCCGCGTACGACGCGGCCGTCGCGGCCGTCGAAGCGGGCACGTTCTCGCTGCGCGTGCGCCCCGTGAA
ATTCTCGCTCGACGCATTCCGGCGCGATCCCGATGCGTACAACCGTTCTCTCGTCGAGGTGCTGTATGCAAGCTGA

Upstream 100 bases:

>100_bases
GTGCGGCGCTCGACGCGGCGGCCGCCGCCGCGAGCCGGTCCGACTCCGAATCCGCAAACCTCAACGTAAACGCAGACGCG
ACACGCGTCGGGGAGGCGCT

Downstream 100 bases:

>100_bases
CGCGCACAAGCTGATCGAGGTCGTGAAGCCCGGCCTCGCGACCTCGGTCCAGGATATGGGCCGGCAAGGCTACTACCACG
TCGGCATTCCGCCGTCGGGC

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTTRYTFGGDEFVFVEISEAMSLDAFFKGTAITRELQRRAVPGITEICPANASYQVRYDPDTIAPDALVALLKSIEADVG
EASLDLDTRIVEVPVLYNDPWTHETMMRFRERHQDPSSTDLEYAARINGKRDVDDFIAAHSGAPWFVSMVGFVAGLPFMY
QMVERERQLEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPAPIFDPEQRLDYLRDFMVFFRPGDIVKFQ
PIDRAAYDAAVAAVEAGTFSLRVRPVKFSLDAFRRDPDAYNRSLVEVLYAS

Sequences:

>Translated_291_residues
MTTRYTFGGDEFVFVEISEAMSLDAFFKGTAITRELQRRAVPGITEICPANASYQVRYDPDTIAPDALVALLKSIEADVG
EASLDLDTRIVEVPVLYNDPWTHETMMRFRERHQDPSSTDLEYAARINGKRDVDDFIAAHSGAPWFVSMVGFVAGLPFMY
QMVERERQLEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPAPIFDPEQRLDYLRDFMVFFRPGDIVKFQ
PIDRAAYDAAVAAVEAGTFSLRVRPVKFSLDAFRRDPDAYNRSLVEVLYAS
>Mature_290_residues
TTRYTFGGDEFVFVEISEAMSLDAFFKGTAITRELQRRAVPGITEICPANASYQVRYDPDTIAPDALVALLKSIEADVGE
ASLDLDTRIVEVPVLYNDPWTHETMMRFRERHQDPSSTDLEYAARINGKRDVDDFIAAHSGAPWFVSMVGFVAGLPFMYQ
MVERERQLEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPAPIFDPEQRLDYLRDFMVFFRPGDIVKFQP
IDRAAYDAAVAAVEAGTFSLRVRPVKFSLDAFRRDPDAYNRSLVEVLYAS

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=235, Percent_Identity=27.2340425531915, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=285, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 32761; Mature: 32629

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYTFGGDEFVFVEISEAMSLDAFFKGTAITRELQRRAVPGITEICPANASYQVRYDP
CCCEEECCCCEEEEEEEHHHHCHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEECC
DTIAPDALVALLKSIEADVGEASLDLDTRIVEVPVLYNDPWTHETMMRFRERHQDPSSTD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
LEYAARINGKRDVDDFIAAHSGAPWFVSMVGFVAGLPFMYQMVERERQLEVPKYLRPRTD
CHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCCC
TPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPAPIFDPEQRLDYLRDFMVFFRPGDIVKFQ
CCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
PIDRAAYDAAVAAVEAGTFSLRVRPVKFSLDAFRRDPDAYNRSLVEVLYAS
CCCHHHHHHHHHHEECCEEEEEEEEEEEEHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTRYTFGGDEFVFVEISEAMSLDAFFKGTAITRELQRRAVPGITEICPANASYQVRYDP
CCEEECCCCEEEEEEEHHHHCHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEECC
DTIAPDALVALLKSIEADVGEASLDLDTRIVEVPVLYNDPWTHETMMRFRERHQDPSSTD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
LEYAARINGKRDVDDFIAAHSGAPWFVSMVGFVAGLPFMYQMVERERQLEVPKYLRPRTD
CHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCCC
TPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPAPIFDPEQRLDYLRDFMVFFRPGDIVKFQ
CCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
PIDRAAYDAAVAAVEAGTFSLRVRPVKFSLDAFRRDPDAYNRSLVEVLYAS
CCCHHHHHHHHHHEECCEEEEEEEEEEEEHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]