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The map label for this gene is pycA [H]

Identifier: 161523547

GI number: 161523547

Start: 404079

End: 405569

Strand: Reverse

Name: pycA [H]

Synonym: Bmul_0367

Alternate gene names: 161523547

Gene position: 405569-404079 (Counterclockwise)

Preceding gene: 161523548

Following gene: 161523546

Centisome position: 11.76

GC content: 71.03

Gene sequence:

>1491_bases
ATGAATCGGGCAACTTCCTGCGACGCGTTCTATCGCCCGTCGCGCATCCGCACCGTGCTGGTCGCGAACCGCGGCGAAAT
CGCGGTGCGCGTGATTCGCGCCGCGCACGAGCTCGGAATGCGCGCGGTCGCCGTGGTCAGCGATGCGGACCGCGACAGCC
TCGCCGCGCGCATGGCCGACGAAGCGATCCACATCGGCTCCTCGCACGCCGCGAAGAGCTACCTGAATCCGGCCGCGATC
CTCGACGCCGCACGGCAATGCGGCGCGGATGCGATCCATCCGGGCTACGGCTTCCTCTCCGAGAACGCCGCATTCGCCGC
GCAGGTCGAAGCGGCCGGCCTCGTGTTCGTCGGCCCGGACGCGCAGGTGATCGCGACGATGGGCGACAAGGCGCGTGCAC
GCGACACCGCGCGGCGCGCGAACGTGCCGACGGTGCCCGGCAGCGACGGCGTCGTGCAGTCGCTCGACGCCGCGCGCGAG
GTGGCCGCGCGCATCGGCTATCCGGTCATGATCAAGGCGGCCGCCGGCGGCGGCGGGCGCGGCATTCGCGTCGCGCACGA
CGCGGCGCAGCTCGAGGCCGAGCTGCCGCTCGCGCAGCGCGAGGCGCAGGCCGCGTTCGGCGACGGCGGCGTCTATCTGG
AGCGCTTCATCGCGCGCGCGCGGCACATCGAAGTACAGGTGCTCGGCGACGGGCGCGACGTCGTCCATCTGTTCGAACGC
GAATGCTCACTGCAGCGCCGCCGCCAGAAGATTCTCGAGGAAGCGCCGTCGCCCTCGCTGACGCCGGCGCTGCGCGCCGA
GCTGTGCGCATCGGCGACGCGTCTCGCGCGCGAAGTCGGCTATCGCAGTGCGGGCACGCTCGAATACCTGTTCGACGACA
CGCGCGGCGAGTTCTACTTCATCGAGATGAACACGCGCATCCAGGTCGAGCATCCGGTGACCGACGCGATCACCGGCATC
GACCTCGTGCGCGAAACGCTGCGCATCGCCGACGGCGAGCCGCTGCGCTTCGCACAGCAGGACATCGCGATGCGCGGCGC
GGCGCTCGAATGCCGGATCAATGCGGAAGACCCGCTGCAGGACTTCCGCCCGAATCCCGGCCGGATCGATGCGCTCGTAT
GGCCGACCGGCCCCGGCGTGCGTGTCGACTCGCTGCTCTATCCGGGCTGCGTCGTGCCGCCGTTCTACGACTCGCTGCTC
GCAAAGCTGATCGTGCACGATGAGAGCCGGGCAGCGGCGCTGCAGCGTCTGTCGCGCGCGCTCGGCGAGCTGCAGGTCGG
CGGCATGAAGACCACCGCGCCGCTGCACGCCGCGCTGCTCGCGGACGCCGACGTCCGCGCCGGCCGCTATCACACGAATT
TCCTCGAGGCGTGGATGCCGCGATGGCGTGCGGCGCTCGACGCGGCGGCCGCCGCCGCGAGCCGGTCCGACTCCGAATCC
GCAAACCTCAACGTAAACGCAGACGCGACACGCGTCGGGGAGGCGCTGTGA

Upstream 100 bases:

>100_bases
ATCGAGTCCGAGGTGCCGGGGCGCGTCGTCGAGCTGCTCGTCGAAGACGGCGAGCCCGTCGACGCGGGGCAGGTGCTGAT
GCGGATCGAGGGGTGACGCG

Downstream 100 bases:

>100_bases
CGACCCGCTATACGTTCGGCGGCGACGAGTTCGTGTTCGTCGAGATCAGCGAAGCGATGTCGCTCGACGCCTTCTTCAAG
GGCACCGCGATCACGCGCGA

Product: acetyl-CoA carboxylase biotin carboxylase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNRATSCDAFYRPSRIRTVLVANRGEIAVRVIRAAHELGMRAVAVVSDADRDSLAARMADEAIHIGSSHAAKSYLNPAAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLVFVGPDAQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDAARE
VAARIGYPVMIKAAAGGGGRGIRVAHDAAQLEAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRDVVHLFER
ECSLQRRRQKILEEAPSPSLTPALRAELCASATRLAREVGYRSAGTLEYLFDDTRGEFYFIEMNTRIQVEHPVTDAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRVDSLLYPGCVVPPFYDSLL
AKLIVHDESRAAALQRLSRALGELQVGGMKTTAPLHAALLADADVRAGRYHTNFLEAWMPRWRAALDAAAAAASRSDSES
ANLNVNADATRVGEAL

Sequences:

>Translated_496_residues
MNRATSCDAFYRPSRIRTVLVANRGEIAVRVIRAAHELGMRAVAVVSDADRDSLAARMADEAIHIGSSHAAKSYLNPAAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLVFVGPDAQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDAARE
VAARIGYPVMIKAAAGGGGRGIRVAHDAAQLEAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRDVVHLFER
ECSLQRRRQKILEEAPSPSLTPALRAELCASATRLAREVGYRSAGTLEYLFDDTRGEFYFIEMNTRIQVEHPVTDAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRVDSLLYPGCVVPPFYDSLL
AKLIVHDESRAAALQRLSRALGELQVGGMKTTAPLHAALLADADVRAGRYHTNFLEAWMPRWRAALDAAAAAASRSDSES
ANLNVNADATRVGEAL
>Mature_496_residues
MNRATSCDAFYRPSRIRTVLVANRGEIAVRVIRAAHELGMRAVAVVSDADRDSLAARMADEAIHIGSSHAAKSYLNPAAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLVFVGPDAQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDAARE
VAARIGYPVMIKAAAGGGGRGIRVAHDAAQLEAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRDVVHLFER
ECSLQRRRQKILEEAPSPSLTPALRAELCASATRLAREVGYRSAGTLEYLFDDTRGEFYFIEMNTRIQVEHPVTDAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRVDSLLYPGCVVPPFYDSLL
AKLIVHDESRAAALQRLSRALGELQVGGMKTTAPLHAALLADADVRAGRYHTNFLEAWMPRWRAALDAAAAAASRSDSES
ANLNVNADATRVGEAL

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=445, Percent_Identity=47.1910112359551, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI65506442, Length=445, Percent_Identity=47.1910112359551, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI295821183, Length=445, Percent_Identity=47.1910112359551, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI106049528, Length=452, Percent_Identity=45.1327433628319, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI106049295, Length=452, Percent_Identity=45.1327433628319, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI106049292, Length=452, Percent_Identity=45.1327433628319, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI116805327, Length=442, Percent_Identity=44.5701357466063, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI38679974, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679971, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679960, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679977, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679967, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI134142062, Length=504, Percent_Identity=31.3492063492063, Blast_Score=216, Evalue=6e-56,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=50.5643340857788, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1786216, Length=284, Percent_Identity=24.2957746478873, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71987519, Length=455, Percent_Identity=47.2527472527472, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567343, Length=459, Percent_Identity=45.0980392156863, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17562816, Length=451, Percent_Identity=44.3458980044346, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133931226, Length=486, Percent_Identity=27.9835390946502, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71997168, Length=509, Percent_Identity=28.2907662082515, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71997163, Length=509, Percent_Identity=28.2907662082515, Blast_Score=189, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=42.9864253393665, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319695, Length=457, Percent_Identity=43.5448577680525, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6321376, Length=457, Percent_Identity=43.7636761487965, Blast_Score=346, Evalue=4e-96,
Organism=Saccharomyces cerevisiae, GI6324343, Length=511, Percent_Identity=31.5068493150685, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323863, Length=465, Percent_Identity=30.9677419354839, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI281363050, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652224, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652222, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652220, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652218, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652212, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652210, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652214, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI19921944, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652216, Length=467, Percent_Identity=45.3961456102784, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24651757, Length=455, Percent_Identity=44.1758241758242, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24651759, Length=405, Percent_Identity=42.962962962963, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI161076409, Length=502, Percent_Identity=31.0756972111554, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI24586458, Length=502, Percent_Identity=31.0756972111554, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI161076407, Length=502, Percent_Identity=31.0756972111554, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24586460, Length=502, Percent_Identity=31.0756972111554, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53309; Mature: 53309

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRATSCDAFYRPSRIRTVLVANRGEIAVRVIRAAHELGMRAVAVVSDADRDSLAARMAD
CCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
EAIHIGSSHAAKSYLNPAAILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLVFVGPD
HHHCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCC
AQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDAAREVAARIGYPVMIKAAAGGGGR
CEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLEAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRDVVHLFER
CEEEECHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHH
ECSLQRRRQKILEEAPSPSLTPALRAELCASATRLAREVGYRSAGTLEYLFDDTRGEFYF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
IEMNTRIQVEHPVTDAITGIDLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQ
EEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
DFRPNPGRIDALVWPTGPGVRVDSLLYPGCVVPPFYDSLLAKLIVHDESRAAALQRLSRA
HCCCCCCCEEEEEECCCCCCEECCEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGELQVGGMKTTAPLHAALLADADVRAGRYHTNFLEAWMPRWRAALDAAAAAASRSDSES
HHHEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ANLNVNADATRVGEAL
CCEEECCCHHHHCCCC
>Mature Secondary Structure
MNRATSCDAFYRPSRIRTVLVANRGEIAVRVIRAAHELGMRAVAVVSDADRDSLAARMAD
CCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
EAIHIGSSHAAKSYLNPAAILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLVFVGPD
HHHCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCC
AQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDAAREVAARIGYPVMIKAAAGGGGR
CEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLEAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRDVVHLFER
CEEEECHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHH
ECSLQRRRQKILEEAPSPSLTPALRAELCASATRLAREVGYRSAGTLEYLFDDTRGEFYF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
IEMNTRIQVEHPVTDAITGIDLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQ
EEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
DFRPNPGRIDALVWPTGPGVRVDSLLYPGCVVPPFYDSLLAKLIVHDESRAAALQRLSRA
HCCCCCCCEEEEEECCCCCCEECCEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGELQVGGMKTTAPLHAALLADADVRAGRYHTNFLEAWMPRWRAALDAAAAAASRSDSES
HHHEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ANLNVNADATRVGEAL
CCEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]