Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 161509594

GI number: 161509594

Start: 1465356

End: 1466312

Strand: Reverse

Name: thyA [H]

Synonym: USA300HOU_1364

Alternate gene names: 161509594

Gene position: 1466312-1465356 (Counterclockwise)

Preceding gene: 161509595

Following gene: 161509593

Centisome position: 51.04

GC content: 33.86

Gene sequence:

>957_bases
ATGTTGAATTCATTTGATGCAGCATATCACAGTCTTTGTGAAGAAGTTTTAGAAATAGGAAATACACGAAATGATCGCAC
AAATACAGGTACGATTTCGAAATTTGGTCATCAACTTCGCTTTGACTTATCTAAAGGATTTCCACTATTAACGACAAAGA
AAGTTTCTTTTAAATTAGTAGCAACCGAATTATTATGGTTCATTAAAGGAGATACAAACATCCAATACTTATTAAAATAT
AATAATAATATATGGAACGAATGGGCTTTTGAAAATTATATCAAATCAGACGAGTATAAAGGTCCAGATATGACAGATTT
CGGGCATCGTGCATTGAGTGATCCTGAATTTAACGAACAATATAAAGAACAAATGAAACAATTTAAGCAACGTATTCTTG
AAGATGATACATTTGCGAAGCAATTCGGGGATTTAGGAAATGTTTATGGTAAACAATGGCGAGATTGGGTTGATAAAGAT
GGTAATCATTTTGATCAACTTAAAACAGTAATTGAACAAATTAAGCATAATCCAGATTCAAGGCGACACATCGTATCTGC
ATGGAATCCAACAGAAATTGATACAATGGCACTTCCGCCTTGTCATACCATGTTCCAGTTTTATGTCCAAGATGGTAAGT
TAAGTTGCCAGTTATACCAACGTAGCGCAGATATCTTTTTAGGTGTGCCATTTAATATCGCAAGCTACGCTTTATTGACA
CACCTTATTGCCAAAGAATGTGGACTTGAAGTGGGTGAATTTGTGCATACATTTGGAGATGCACATATTTATTCAAATCA
TATTGATGCGATTCAAACACAATTAGCACGTGAAAGCTTCAATCCTCCAACATTAAAAATTAACAGTGACAAGTCTATTT
TCGACATAAATTATGAAGATTTGGAAATTGTTGACTATGAATCACATCCAGCAATAAAAGCTCCAATAGCAGTGTAG

Upstream 100 bases:

>100_bases
CATATTCCTAAAATGAGTGATAACAAAATGCTACATAAAGTTAAGTTATATCAAACTAAATATACATACTATAAATAATG
AAAATGAGGTGTTATCGCAT

Downstream 100 bases:

>100_bases
TCATTGCATAGTTAGCTAACCATATAGACATCAAAATGACATCATAGTATTTTCAAGTGCAAAAAAGTACTTTTTTGTGT
TAAACGTTTTCATAAATTAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

Sequences:

>Translated_318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV
>Mature_318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=319, Percent_Identity=43.5736677115987, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1789191, Length=311, Percent_Identity=50.1607717041801, Blast_Score=307, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI71993377, Length=327, Percent_Identity=45.565749235474, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=197, Percent_Identity=51.7766497461929, Blast_Score=190, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17137556, Length=328, Percent_Identity=43.9024390243902, Blast_Score=260, Evalue=9e-70,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36840; Mature: 36840

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECCHHHHHH
ATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQ
HHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHH
YKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
RRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHH
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYED
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCC
LEIVDYESHPAIKAPIAV
EEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECCHHHHHH
ATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQ
HHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHH
YKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
RRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHH
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYED
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCC
LEIVDYESHPAIKAPIAV
EEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA