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The map label for this gene is dfrB

Identifier: 161509593

GI number: 161509593

Start: 1464677

End: 1465156

Strand: Reverse

Name: dfrB

Synonym: USA300HOU_1363

Alternate gene names: 161509593

Gene position: 1465156-1464677 (Counterclockwise)

Preceding gene: 161509594

Following gene: 161509592

Centisome position: 51.0

GC content: 34.58

Gene sequence:

>480_bases
ATGACTTTATCCATTCTAGTTGCACATGACTTGCAACGAGTAATTGGTTTTGAAAATCAATTACCTTGGCACCTACCAAA
TGATTTGAAGCATGTTAAAAAATTATCAACAGGTCATACTTTAGTAATGGGTCGTAAGACATTTGAATCGATTGGTAAAC
CACTACCGAATCGTCGAAATGTTGTACTTACTTCAGATACAAGTTTCAACGTAGAGGGCGTTGATGTAATTCACTCTATT
GAAGATATTTACCAACTACCGGGCCATGTTTTCATATTTGGAGGGCAAACATTATTTGAAGAAATGATTGATAAAGTGGA
CGACATGTATATTACTGTTATTGAAGGTAAATTCCGTGGTGATACGTTCTTTCCACCTTATACATTTGAAGACTGGGAAG
TTGCCTCTTCAGTTGAAGGTAAACTAGATGAGAAAAATACAATTCCACATACCTTTCTACATTTAATTCGTAAAAAATAA

Upstream 100 bases:

>100_bases
TGCAAAATCATTATTTCTATCACACTTTATGATAAAAATTGTGTTAAATTAAAGATAACTTAGTAATAAAAAATGAAATG
ATAGAAGAAGGAGGATAATT

Downstream 100 bases:

>100_bases
GGGGGAAAACGACCATGACAAAACAGATTATAGTAACAGACTCAACATCCGATTTATCTAAAGAATACTTAGAAGCAAAC
AACATTCATGTAATTCCTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: DHFR

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSI
EDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK

Sequences:

>Translated_159_residues
MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSI
EDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
>Mature_158_residues
TLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIE
DIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=35.2201257861635, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_STAAC (Q5HFZ7)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186310.1
- ProteinModelPortal:   Q5HFZ7
- SMR:   Q5HFZ7
- STRING:   Q5HFZ7
- EnsemblBacteria:   EBSTAT00000008807
- GeneID:   3238319
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1461
- TIGR:   SACOL1461
- eggNOG:   COG0262
- GeneTree:   EBGT00050000024106
- HOGENOM:   HBG665708
- OMA:   QDEEGVI
- ProtClustDB:   CLSK885344
- BioCyc:   SAUR93062:SACOL1461-MONOMER
- BindingDB:   Q5HFZ7
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 18251; Mature: 18120

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 28-28 BINDING 58-58 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
CEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCC
VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCC
DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
EEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCC
VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCC
DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA