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The map label for this gene is ispD

Identifier: 160902676

GI number: 160902676

Start: 1302208

End: 1302891

Strand: Direct

Name: ispD

Synonym: Pmob_1218

Alternate gene names: 160902676

Gene position: 1302208-1302891 (Clockwise)

Preceding gene: 160902674

Following gene: 160902700

Centisome position: 60.02

GC content: 33.19

Gene sequence:

>684_bases
ATGGTCTATGCAATTATAGTAGCAGCTGGAGAAGGAAAAAGAGCGGGATTTGAAATTCCAAAACAATTTGTAAAATTAAA
TAACAAAACCATATTGAGAATGTCAGCTGAAAAATTCCAGAATTCAGCATCAATAGATAAGTTTCTCGTCGTATCTCACA
GAAACTACGTGGATTTAACAGAAAAAGAAGTTAAAATCTTTTCAAAATTTGAGAATGTTGTAATTGGCGGAAGTAACAGG
CAAGAAAGTGTTTATAATGCCCTAATGTATTTGGGCAAAAAAGAGAATAAGCCTGATTTTGTTTGCATTCATGATGCCGT
CAGACCTTTCGTTGATACTAATAAAATAGACGAAAGCATCTACAAAGCAAAAGAGATAGGCGGGGCTGTATTAGCAGAAA
TGGCTGAAAATACAATTTCACAGGTGAATAATGGAAGGATTGTAAAAACTTTAGAAAGATCCCAAATTTATCTTCACCAT
ACCCCTCAAACTTTTGATTTCAATAAACTACTCAAAGCTTACCAAAAAGCAGAAAAGATCCTATCTTCTTTTACCGATGA
TGCCTCTATATTTATCCATGCGGGTTATGAAACGGCGATTGTGGAAGATTACAAAAATAACATTAAGCTCACAAAAAAAG
AGGATTTTGAACTTGCAAAATGTATTTTTGAATTAAATTCTTAG

Upstream 100 bases:

>100_bases
CAATCTATTATATCAAAAGTTAAATTAATTATTTAAAAAAAAGATGAAGTTCTACTAAATCTATTTTTTAAAATACTACA
AATAATAAAGGGTGAAGAAA

Downstream 100 bases:

>100_bases
TCTAAAAATGTTTCAAAAACCTGGATCCCAACATCTATTACCTCATCGGGAGGCAAAAATTCAGGGTTATGAAGACCATG
GTGTTCACCTTTAGAACTTC

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MVYAIIVAAGEGKRAGFEIPKQFVKLNNKTILRMSAEKFQNSASIDKFLVVSHRNYVDLTEKEVKIFSKFENVVIGGSNR
QESVYNALMYLGKKENKPDFVCIHDAVRPFVDTNKIDESIYKAKEIGGAVLAEMAENTISQVNNGRIVKTLERSQIYLHH
TPQTFDFNKLLKAYQKAEKILSSFTDDASIFIHAGYETAIVEDYKNNIKLTKKEDFELAKCIFELNS

Sequences:

>Translated_227_residues
MVYAIIVAAGEGKRAGFEIPKQFVKLNNKTILRMSAEKFQNSASIDKFLVVSHRNYVDLTEKEVKIFSKFENVVIGGSNR
QESVYNALMYLGKKENKPDFVCIHDAVRPFVDTNKIDESIYKAKEIGGAVLAEMAENTISQVNNGRIVKTLERSQIYLHH
TPQTFDFNKLLKAYQKAEKILSSFTDDASIFIHAGYETAIVEDYKNNIKLTKKEDFELAKCIFELNS
>Mature_227_residues
MVYAIIVAAGEGKRAGFEIPKQFVKLNNKTILRMSAEKFQNSASIDKFLVVSHRNYVDLTEKEVKIFSKFENVVIGGSNR
QESVYNALMYLGKKENKPDFVCIHDAVRPFVDTNKIDESIYKAKEIGGAVLAEMAENTISQVNNGRIVKTLERSQIYLHH
TPQTFDFNKLLKAYQKAEKILSSFTDDASIFIHAGYETAIVEDYKNNIKLTKKEDFELAKCIFELNS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=231, Percent_Identity=25.974025974026, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=27.3542600896861, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PETMO (A9BHR2)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568257.1
- ProteinModelPortal:   A9BHR2
- SMR:   A9BHR2
- GeneID:   5756693
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1218
- HOGENOM:   HBG672839
- OMA:   REQQDFW
- BioCyc:   PMOB403833:PMOB_1218-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25818; Mature: 25818

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYAIIVAAGEGKRAGFEIPKQFVKLNNKTILRMSAEKFQNSASIDKFLVVSHRNYVDLT
CEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCEEEEEECCCCCCCC
EKEVKIFSKFENVVIGGSNRQESVYNALMYLGKKENKPDFVCIHDAVRPFVDTNKIDESI
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCHHHHHHH
YKAKEIGGAVLAEMAENTISQVNNGRIVKTLERSQIYLHHTPQTFDFNKLLKAYQKAEKI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHH
LSSFTDDASIFIHAGYETAIVEDYKNNIKLTKKEDFELAKCIFELNS
HHHCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MVYAIIVAAGEGKRAGFEIPKQFVKLNNKTILRMSAEKFQNSASIDKFLVVSHRNYVDLT
CEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCEEEEEECCCCCCCC
EKEVKIFSKFENVVIGGSNRQESVYNALMYLGKKENKPDFVCIHDAVRPFVDTNKIDESI
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCHHHHHHH
YKAKEIGGAVLAEMAENTISQVNNGRIVKTLERSQIYLHHTPQTFDFNKLLKAYQKAEKI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHH
LSSFTDDASIFIHAGYETAIVEDYKNNIKLTKKEDFELAKCIFELNS
HHHCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA