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The map label for this gene is proS

Identifier: 160902700

GI number: 160902700

Start: 1327839

End: 1329587

Strand: Direct

Name: proS

Synonym: Pmob_1242

Alternate gene names: 160902700

Gene position: 1327839-1329587 (Clockwise)

Preceding gene: 160902676

Following gene: 160902701

Centisome position: 61.2

GC content: 36.76

Gene sequence:

>1749_bases
TTGAGATTTTCCAAACTATATGCTCCTACTTTAAAAGAAACACCATCTGATTCTGATATAAAAAGTTACGAGTTATTGAT
CAGAGGAGGGTTTATTAGAAAGATCTCATCAGGTGTTTACAGTTATCTTCCCCTTGGATGGAAGGTCATAAGAAAAATCG
AACAGATAGTCAGAGAAGAAATGGAAAAGATAGGTTCTCAAGAGATCATGCTACCCATAATACATCCAGCAGAGTTGTGG
AAAATGACCGGAAGATGGGAAGATTACGGACCAGAATTGATGAAACTTAAGGACAGACACGATAGAGAGTTTACTTTAGG
TCCCACACATGAAGAAATAGTCACATTTTTGATGAAAAATGAGCTTAGATCGTACAAACAGTTCCCGGTCAACCTTTTCC
AAATAGCCACTAAGTTTAGAGATGAAATAAGGCCAAGGTTTGGGGTGCTAAGGGCAAGAGAATTCATCATGAAAGACGCC
TACACCTTTCATACCGACTACAACTCATTGCATGAATCTTATCAACATTTCTATAATGCCTATGAAAACATCATAAAAAG
AATAGGTTTAAAATATGTCGTTGTAGAAGCGGATACCGGTGCAATTGGTGGATCTTTTTCTCATGAATTTCATGTATTGG
CGCAAAATGGCGAGGGAGAAATATTTTACTGTGAAAAATGTGGTTATGCAGCCAGTGATGAAAAGGCAAGATCGGGTGAA
GATTTTTCAGTAGACGAAAATGAAGCCTTCAAAGAAATGGAAAAAGTAGACACCCAACACGCCAAAACCATCGAAGAAGT
CGCCAAATTCCTGAATGTTTCTGAAAAGAAGTTGATAAAATCTATACTGCTAAGATCCAGCAAAGGATGGGTGATGGCAC
TAATTAGAGGAGATTATGAGATAAACCTCGCAAAGGTCAGATCGGTTCTTCAAGATCAAAGCTTAGAACTAGCCGATCCG
CAAGACGTTCTTAAAGAATTTGGTGTAAACGTTGGATTTATAGGCCCAATAAACATACCTGAAAATGTCAAGATTATTGC
AGATCTAAGTGTAAAATCCATAAAAAATGGGGTTATAGGGGCTATGGAAGAAAAAGCCCATTACATCAATGCAAACGCCG
AAAGAGATTTCAGAATAAATTTATTTGCAGACATTAGATACGTAAAAGAAGGCGAAAAATGTCCAACCCAAGGATGTAAT
TCTACACTCAAACGAACAAGAGGCATAGAAGTTGGTCAAATTTTTGAATTAGGAGACAAATACTCTTCAAAAATGAACGC
AGTCTTCACCGATGAAAATGGAGAACAAAAACCTTACATAATGGGATGCTATGGATGGGGGGTATCAAGAACCTTAGGGG
CAATAGTCGAACAATTAAACGATGAAAACGGAATAATCTGGCCAAAAAGCGTTGCGCCTTTTGAAGTGGCAATAATACCG
GTTGCCATGAACGACGAAGCAATTGTAAGCACATCGCAAGAGATCTACGATTACTTTTTAAAAGAAGGAATCGACGTCAT
TATAGACGATAGAGAAGTCTCCGCTGGATTTAAGTTCAAAGATATAGATCTAATCGGAGTACCGATAAAAATTATTATCG
GCAAACGTTTAAAAGAAGGAAAAATTGAGTTAAAACAAAGAGATAAAGAAGAAGGCACATTGATAGAGTTCAAAAATGTA
AAAGAGTTATACGATAAAGTAAAAGAAAAATTAGAAAACTATGATCCAACTCAAAATTTAACAATCTAA

Upstream 100 bases:

>100_bases
ATATTTTATTACAGTAAAAATTTAACTTAACAATATTTCTATGATATAATTTCTCAAAGTACAAAAAATTAAATATGCTA
AAAAAATCGGAGGGATAATC

Downstream 100 bases:

>100_bases
CACATTCCTTATGGGCGGGCAGCGGGGGAAGGGCGCTAATAAAATTATTAAAAAATATCACTCCTCTTTTTAGTTTTATG
AGGAGTGATATCTCTATTAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MRFSKLYAPTLKETPSDSDIKSYELLIRGGFIRKISSGVYSYLPLGWKVIRKIEQIVREEMEKIGSQEIMLPIIHPAELW
KMTGRWEDYGPELMKLKDRHDREFTLGPTHEEIVTFLMKNELRSYKQFPVNLFQIATKFRDEIRPRFGVLRAREFIMKDA
YTFHTDYNSLHESYQHFYNAYENIIKRIGLKYVVVEADTGAIGGSFSHEFHVLAQNGEGEIFYCEKCGYAASDEKARSGE
DFSVDENEAFKEMEKVDTQHAKTIEEVAKFLNVSEKKLIKSILLRSSKGWVMALIRGDYEINLAKVRSVLQDQSLELADP
QDVLKEFGVNVGFIGPINIPENVKIIADLSVKSIKNGVIGAMEEKAHYINANAERDFRINLFADIRYVKEGEKCPTQGCN
STLKRTRGIEVGQIFELGDKYSSKMNAVFTDENGEQKPYIMGCYGWGVSRTLGAIVEQLNDENGIIWPKSVAPFEVAIIP
VAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEGKIELKQRDKEEGTLIEFKNV
KELYDKVKEKLENYDPTQNLTI

Sequences:

>Translated_582_residues
MRFSKLYAPTLKETPSDSDIKSYELLIRGGFIRKISSGVYSYLPLGWKVIRKIEQIVREEMEKIGSQEIMLPIIHPAELW
KMTGRWEDYGPELMKLKDRHDREFTLGPTHEEIVTFLMKNELRSYKQFPVNLFQIATKFRDEIRPRFGVLRAREFIMKDA
YTFHTDYNSLHESYQHFYNAYENIIKRIGLKYVVVEADTGAIGGSFSHEFHVLAQNGEGEIFYCEKCGYAASDEKARSGE
DFSVDENEAFKEMEKVDTQHAKTIEEVAKFLNVSEKKLIKSILLRSSKGWVMALIRGDYEINLAKVRSVLQDQSLELADP
QDVLKEFGVNVGFIGPINIPENVKIIADLSVKSIKNGVIGAMEEKAHYINANAERDFRINLFADIRYVKEGEKCPTQGCN
STLKRTRGIEVGQIFELGDKYSSKMNAVFTDENGEQKPYIMGCYGWGVSRTLGAIVEQLNDENGIIWPKSVAPFEVAIIP
VAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEGKIELKQRDKEEGTLIEFKNV
KELYDKVKEKLENYDPTQNLTI
>Mature_582_residues
MRFSKLYAPTLKETPSDSDIKSYELLIRGGFIRKISSGVYSYLPLGWKVIRKIEQIVREEMEKIGSQEIMLPIIHPAELW
KMTGRWEDYGPELMKLKDRHDREFTLGPTHEEIVTFLMKNELRSYKQFPVNLFQIATKFRDEIRPRFGVLRAREFIMKDA
YTFHTDYNSLHESYQHFYNAYENIIKRIGLKYVVVEADTGAIGGSFSHEFHVLAQNGEGEIFYCEKCGYAASDEKARSGE
DFSVDENEAFKEMEKVDTQHAKTIEEVAKFLNVSEKKLIKSILLRSSKGWVMALIRGDYEINLAKVRSVLQDQSLELADP
QDVLKEFGVNVGFIGPINIPENVKIIADLSVKSIKNGVIGAMEEKAHYINANAERDFRINLFADIRYVKEGEKCPTQGCN
STLKRTRGIEVGQIFELGDKYSSKMNAVFTDENGEQKPYIMGCYGWGVSRTLGAIVEQLNDENGIIWPKSVAPFEVAIIP
VAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEGKIELKQRDKEEGTLIEFKNV
KELYDKVKEKLENYDPTQNLTI

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.0353982300885, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=43.9579684763573, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1788013, Length=122, Percent_Identity=27.0491803278689, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115532348, Length=211, Percent_Identity=39.8104265402844, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=38.8235294117647, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=535, Percent_Identity=30.0934579439252, Blast_Score=237, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24656200, Length=319, Percent_Identity=32.9153605015674, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PETMO (A9BGA8)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568281.1
- ProteinModelPortal:   A9BGA8
- SMR:   A9BGA8
- GeneID:   5756638
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1242
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   PMOB403833:PMOB_1242-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66676; Mature: 66676

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSKLYAPTLKETPSDSDIKSYELLIRGGFIRKISSGVYSYLPLGWKVIRKIEQIVREE
CCCCHHCCCCCCCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MEKIGSQEIMLPIIHPAELWKMTGRWEDYGPELMKLKDRHDREFTLGPTHEEIVTFLMKN
HHHCCCCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
ELRSYKQFPVNLFQIATKFRDEIRPRFGVLRAREFIMKDAYTFHTDYNSLHESYQHFYNA
HHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHH
YENIIKRIGLKYVVVEADTGAIGGSFSHEFHVLAQNGEGEIFYCEKCGYAASDEKARSGE
HHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
DFSVDENEAFKEMEKVDTQHAKTIEEVAKFLNVSEKKLIKSILLRSSKGWVMALIRGDYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCE
INLAKVRSVLQDQSLELADPQDVLKEFGVNVGFIGPINIPENVKIIADLSVKSIKNGVIG
EEHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHHCCCCH
AMEEKAHYINANAERDFRINLFADIRYVKEGEKCPTQGCNSTLKRTRGIEVGQIFELGDK
HHHHHHHEECCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
YSSKMNAVFTDENGEQKPYIMGCYGWGVSRTLGAIVEQLNDENGIIWPKSVAPFEVAIIP
HCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEE
VAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEG
EEECCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCCEEEECCCCCCC
KIELKQRDKEEGTLIEFKNVKELYDKVKEKLENYDPTQNLTI
CHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFSKLYAPTLKETPSDSDIKSYELLIRGGFIRKISSGVYSYLPLGWKVIRKIEQIVREE
CCCCHHCCCCCCCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MEKIGSQEIMLPIIHPAELWKMTGRWEDYGPELMKLKDRHDREFTLGPTHEEIVTFLMKN
HHHCCCCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
ELRSYKQFPVNLFQIATKFRDEIRPRFGVLRAREFIMKDAYTFHTDYNSLHESYQHFYNA
HHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHH
YENIIKRIGLKYVVVEADTGAIGGSFSHEFHVLAQNGEGEIFYCEKCGYAASDEKARSGE
HHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
DFSVDENEAFKEMEKVDTQHAKTIEEVAKFLNVSEKKLIKSILLRSSKGWVMALIRGDYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCE
INLAKVRSVLQDQSLELADPQDVLKEFGVNVGFIGPINIPENVKIIADLSVKSIKNGVIG
EEHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHHCCCCH
AMEEKAHYINANAERDFRINLFADIRYVKEGEKCPTQGCNSTLKRTRGIEVGQIFELGDK
HHHHHHHEECCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
YSSKMNAVFTDENGEQKPYIMGCYGWGVSRTLGAIVEQLNDENGIIWPKSVAPFEVAIIP
HCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEE
VAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEG
EEECCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCCEEEECCCCCCC
KIELKQRDKEEGTLIEFKNVKELYDKVKEKLENYDPTQNLTI
CHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA