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The map label for this gene is prfB

Identifier: 160902329

GI number: 160902329

Start: 917459

End: 918559

Strand: Direct

Name: prfB

Synonym: Pmob_0864

Alternate gene names: 160902329

Gene position: 917459-918559 (Clockwise)

Preceding gene: 160902328

Following gene: 160902330

Centisome position: 42.29

GC content: 34.06

Gene sequence:

>1101_bases
ATGATAGAATACGAAACGAAAGTAAAAATAGACGAATTAAAAGAAAATTTTGAAGATTTAAAAGACGTTTTTAATATAGA
TAAAGTAGAAGAAGAAGTCAAAAAACTCGACAAAGAAATGATGGAACCAAACTTTTGGAACGATCAAAATAGAGCTAAAA
AGATCTCCAAAATGGCACAAAATTTAAAAGATGAAATAGATGAATTTAAAAAATTGGAGAACGACTTTGAGGAATTAGAA
ATAGCTGTTGAATTATCAGAGGATGATCCATCAATGACCGCCCAAGTTGAAGCAATTTTAAAAGTTATTGAAAAAAAGAT
CGGTTCTTTTCGATTAAGAATGCTTTTATCAGAAGAATACGATGATGCAAATGCATTTCTGAGTCTACATCCGGGTGCGG
GAGGAACAGAATCACAGGATTGGGCATCTATGCTATTGAGAATGTACACTCGTTGGGCAGATAAAAATAATTATGACATT
GAAACGATAGACTTTCAAGAAGGAGACGAAGCGGGAATAAAAAGTGCAACCATAAAAATTTCAGGCCCCTATGCTTACGG
TAAATTAAAATATGAAAGCGGTGTACATCGACTAGTAAGAATTTCTCCATTTGACGCCAATGGAAGAAGGCACACTTCTT
TCGCCTCAATATCTGTGATGCCTGAATTTGATGAAAATGTTGAGATAGAAATAAATCCAGACGATCTGAAAATAGACACG
TACAGATCAGGTGGAGCAGGTGGACAGCATGTCAATAAAACCGATTCTGCGGTTAGGATTACTCATTTACCAACAGGTAT
AGTTGTAGCTGTACAAAACGAGCGTTCACAACATCAAAACAAAGCCACTGCTTTAAAAATATTAAAGGCTAAATTATATG
AGTTAGAACATCAAAAAAAGTTAGAAGAAAAGTTAAGATTACGTGGTGAGGTAAAAGATATTTCATGGGGAAATCAGATT
AGATCATATGTTTTGTATCCATATACCTTAGTTAAAGATCTAAGAACAGAGTATGAAACCTCTAATGCTCAAGCAGTCTT
GGATGGAGAAATAGACGAATTCATAGAAGAAGAACTGTTATTCTTTGCGAAATATAAGTAA

Upstream 100 bases:

>100_bases
AGAAGCTAAAAGTAAAAATACCGCTAAACAAGCCAACAAGATTAAAAAAAGGTATAAAGTTAAGAGATAATAATTGAAAC
CATTTTTAAGAGGTGATCAG

Downstream 100 bases:

>100_bases
AAAAATTAATTTGTTTTTCTGCAAAAGAGGTGTATAATAATAGTAAGAAAATACGACGATTTTCATAAAAGTGTAATGGT
TGAGGAAGTCTTATCCTATA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MIEYETKVKIDELKENFEDLKDVFNIDKVEEEVKKLDKEMMEPNFWNDQNRAKKISKMAQNLKDEIDEFKKLENDFEELE
IAVELSEDDPSMTAQVEAILKVIEKKIGSFRLRMLLSEEYDDANAFLSLHPGAGGTESQDWASMLLRMYTRWADKNNYDI
ETIDFQEGDEAGIKSATIKISGPYAYGKLKYESGVHRLVRISPFDANGRRHTSFASISVMPEFDENVEIEINPDDLKIDT
YRSGGAGGQHVNKTDSAVRITHLPTGIVVAVQNERSQHQNKATALKILKAKLYELEHQKKLEEKLRLRGEVKDISWGNQI
RSYVLYPYTLVKDLRTEYETSNAQAVLDGEIDEFIEEELLFFAKYK

Sequences:

>Translated_366_residues
MIEYETKVKIDELKENFEDLKDVFNIDKVEEEVKKLDKEMMEPNFWNDQNRAKKISKMAQNLKDEIDEFKKLENDFEELE
IAVELSEDDPSMTAQVEAILKVIEKKIGSFRLRMLLSEEYDDANAFLSLHPGAGGTESQDWASMLLRMYTRWADKNNYDI
ETIDFQEGDEAGIKSATIKISGPYAYGKLKYESGVHRLVRISPFDANGRRHTSFASISVMPEFDENVEIEINPDDLKIDT
YRSGGAGGQHVNKTDSAVRITHLPTGIVVAVQNERSQHQNKATALKILKAKLYELEHQKKLEEKLRLRGEVKDISWGNQI
RSYVLYPYTLVKDLRTEYETSNAQAVLDGEIDEFIEEELLFFAKYK
>Mature_366_residues
MIEYETKVKIDELKENFEDLKDVFNIDKVEEEVKKLDKEMMEPNFWNDQNRAKKISKMAQNLKDEIDEFKKLENDFEELE
IAVELSEDDPSMTAQVEAILKVIEKKIGSFRLRMLLSEEYDDANAFLSLHPGAGGTESQDWASMLLRMYTRWADKNNYDI
ETIDFQEGDEAGIKSATIKISGPYAYGKLKYESGVHRLVRISPFDANGRRHTSFASISVMPEFDENVEIEINPDDLKIDT
YRSGGAGGQHVNKTDSAVRITHLPTGIVVAVQNERSQHQNKATALKILKAKLYELEHQKKLEEKLRLRGEVKDISWGNQI
RSYVLYPYTLVKDLRTEYETSNAQAVLDGEIDEFIEEELLFFAKYK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=268, Percent_Identity=36.1940298507463, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI34577120, Length=253, Percent_Identity=36.3636363636364, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI166795305, Length=170, Percent_Identity=38.8235294117647, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI2367172, Length=355, Percent_Identity=50.9859154929577, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1787462, Length=316, Percent_Identity=38.2911392405063, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=274, Percent_Identity=33.9416058394161, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=344, Percent_Identity=35.1744186046512, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19921226, Length=225, Percent_Identity=38.2222222222222, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_PETMO (A9BHH8)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567910.1
- ProteinModelPortal:   A9BHH8
- SMR:   A9BHH8
- GeneID:   5757124
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0864
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   PMOB403833:PMOB_0864-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 42227; Mature: 42227

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEYETKVKIDELKENFEDLKDVFNIDKVEEEVKKLDKEMMEPNFWNDQNRAKKISKMAQ
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NLKDEIDEFKKLENDFEELEIAVELSEDDPSMTAQVEAILKVIEKKIGSFRLRMLLSEEY
HHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDANAFLSLHPGAGGTESQDWASMLLRMYTRWADKNNYDIETIDFQEGDEAGIKSATIKI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEE
SGPYAYGKLKYESGVHRLVRISPFDANGRRHTSFASISVMPEFDENVEIEINPDDLKIDT
ECCEEEEEEEHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YRSGGAGGQHVNKTDSAVRITHLPTGIVVAVQNERSQHQNKATALKILKAKLYELEHQKK
ECCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEKLRLRGEVKDISWGNQIRSYVLYPYTLVKDLRTEYETSNAQAVLDGEIDEFIEEELL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHH
FFAKYK
HHHCCC
>Mature Secondary Structure
MIEYETKVKIDELKENFEDLKDVFNIDKVEEEVKKLDKEMMEPNFWNDQNRAKKISKMAQ
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NLKDEIDEFKKLENDFEELEIAVELSEDDPSMTAQVEAILKVIEKKIGSFRLRMLLSEEY
HHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDANAFLSLHPGAGGTESQDWASMLLRMYTRWADKNNYDIETIDFQEGDEAGIKSATIKI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEE
SGPYAYGKLKYESGVHRLVRISPFDANGRRHTSFASISVMPEFDENVEIEINPDDLKIDT
ECCEEEEEEEHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YRSGGAGGQHVNKTDSAVRITHLPTGIVVAVQNERSQHQNKATALKILKAKLYELEHQKK
ECCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEKLRLRGEVKDISWGNQIRSYVLYPYTLVKDLRTEYETSNAQAVLDGEIDEFIEEELL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHH
FFAKYK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA