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The map label for this gene is aglA [H]

Identifier: 160902319

GI number: 160902319

Start: 900557

End: 901948

Strand: Direct

Name: aglA [H]

Synonym: Pmob_0853

Alternate gene names: 160902319

Gene position: 900557-901948 (Clockwise)

Preceding gene: 160902315

Following gene: 160902320

Centisome position: 41.51

GC content: 34.27

Gene sequence:

>1392_bases
ATGCCAGTTAAAATTTCTTTTATAGGAGCAGGTAGTGTCAGATACACAGTGAAATTAGTAGGGGATTTAGCAAAAACAAA
GGAATTAAATGGAAGTTTAATCTCTTTCATGGACATAGACGAAGAACGATTAAACGCCGTAGATAACCTCGCTAAAAGAT
ACACGGAAGAAATAGGTGGAAATCTTAAATTTGAGAAAACTACCAACCGTGAAAAATCTTTAAAAGATGCAGATTTTGTT
ATAAACACAGCATTATACAGAGCGCCAGGCCACGAAGACGGTTATGTAAGTTATGAAATCATGAGAGATGTTGCAGAAAA
ATATGGTTACTACAGAGGAATAGATAGTCAAGAGTTCAACATGGTATCGGATTATTATACATTCACAAATTACAATCATC
TGAAATTATCATTAGATATAGCTAAATCGATTGAAAAAATATGTCCGAATGCATGGCTTATACAAACCGCTAATCCTGTT
TTCGAAATTACACAATTGATTAAAAGGCTGACCAACGTAAAAGTAGTAGGCTTTTGTCATGGGGTTGGAGGTGTCCATGA
AGTACTAAAAACTTTACGATTAGAAGAAAACGAAACTGATTGGCAGGTAGCTGGAGTGAACCATGGGATATGGTTAAACC
GATTCCTATACAAGGGCGAAGATGGTTACCAAATCTTAGACAAATGGATAGAAGAAGAATCAAATAATTGGGAACCAAAA
GATCCTTGGGATACCCATTTAGCTCCAGCGGTTATCGATATGTACAAATTCTATGGATTATTGCCTATAGGAGACACAAC
TAGAAACAGTACATGGAAACATCATCATAGTTTACAAGCAAAAAAGAAATGGTATGGAAAGTTCGGGGCTATAGACAATG
AAGTTGAAAGGCCGAAGTTCTATGAAGAATTACGTCAAACAAAAAGAATGATAATTCAAGTTTCGAAAGATCCGAATATC
AAAATAACAGAAAACTGGCCTGAAGAATTCCCAAAAGAAAAAATGAGTGGTGAACAACAAGTGCCTTTTATAAACGCTTT
AACTAATGATGTTGAAGCAAGATTATTCCTGAATGTTTTGAATGATGGAACGATAAAAAACATTCCTAACGATGTAGTTG
TAGAAGTGCCTGTAAAAGTTAACAAAAATGGAATATTCCCTGAAAAGATACAGCCAGATCTACCAGAAAAGATTAAAAAT
TACTATATAATCCCCAGAATCACAAGAATGGAAATGGCATTAGAAGCCTTTATAACTGGAAACAGAAATATCTTAGAAGA
AGTTTTAGTAAGAGATCCAAGAACCAAAAATTATGATGATATTCCTAAGTTATGGGATGAGATATTCGATCTTCCATTTA
ACAAGGAAATGAAGGAACATTATAGAAGTTAA

Upstream 100 bases:

>100_bases
AATCAGGTAAAATCAAGGTTGACTTTTTGTTTATTTTAATTTAAAATGGATACGAATAAATTCGATTTTAGAATGAATTA
ATTAAAGGAGTTAGAAAAAC

Downstream 100 bases:

>100_bases
TAACGTGCTTAAAAGAAAAGGTCCTAAAGCATCTTTTAGAATTTTCAAAGACAGGAAGAATTGAAGTATAGGAGGTTAGA
AAAATGTCTTTTTTGGACGA

Product: Alpha-glucosidase

Products: NA

Alternate protein names: Maltase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPVKISFIGAGSVRYTVKLVGDLAKTKELNGSLISFMDIDEERLNAVDNLAKRYTEEIGGNLKFEKTTNREKSLKDADFV
INTALYRAPGHEDGYVSYEIMRDVAEKYGYYRGIDSQEFNMVSDYYTFTNYNHLKLSLDIAKSIEKICPNAWLIQTANPV
FEITQLIKRLTNVKVVGFCHGVGGVHEVLKTLRLEENETDWQVAGVNHGIWLNRFLYKGEDGYQILDKWIEEESNNWEPK
DPWDTHLAPAVIDMYKFYGLLPIGDTTRNSTWKHHHSLQAKKKWYGKFGAIDNEVERPKFYEELRQTKRMIIQVSKDPNI
KITENWPEEFPKEKMSGEQQVPFINALTNDVEARLFLNVLNDGTIKNIPNDVVVEVPVKVNKNGIFPEKIQPDLPEKIKN
YYIIPRITRMEMALEAFITGNRNILEEVLVRDPRTKNYDDIPKLWDEIFDLPFNKEMKEHYRS

Sequences:

>Translated_463_residues
MPVKISFIGAGSVRYTVKLVGDLAKTKELNGSLISFMDIDEERLNAVDNLAKRYTEEIGGNLKFEKTTNREKSLKDADFV
INTALYRAPGHEDGYVSYEIMRDVAEKYGYYRGIDSQEFNMVSDYYTFTNYNHLKLSLDIAKSIEKICPNAWLIQTANPV
FEITQLIKRLTNVKVVGFCHGVGGVHEVLKTLRLEENETDWQVAGVNHGIWLNRFLYKGEDGYQILDKWIEEESNNWEPK
DPWDTHLAPAVIDMYKFYGLLPIGDTTRNSTWKHHHSLQAKKKWYGKFGAIDNEVERPKFYEELRQTKRMIIQVSKDPNI
KITENWPEEFPKEKMSGEQQVPFINALTNDVEARLFLNVLNDGTIKNIPNDVVVEVPVKVNKNGIFPEKIQPDLPEKIKN
YYIIPRITRMEMALEAFITGNRNILEEVLVRDPRTKNYDDIPKLWDEIFDLPFNKEMKEHYRS
>Mature_462_residues
PVKISFIGAGSVRYTVKLVGDLAKTKELNGSLISFMDIDEERLNAVDNLAKRYTEEIGGNLKFEKTTNREKSLKDADFVI
NTALYRAPGHEDGYVSYEIMRDVAEKYGYYRGIDSQEFNMVSDYYTFTNYNHLKLSLDIAKSIEKICPNAWLIQTANPVF
EITQLIKRLTNVKVVGFCHGVGGVHEVLKTLRLEENETDWQVAGVNHGIWLNRFLYKGEDGYQILDKWIEEESNNWEPKD
PWDTHLAPAVIDMYKFYGLLPIGDTTRNSTWKHHHSLQAKKKWYGKFGAIDNEVERPKFYEELRQTKRMIIQVSKDPNIK
ITENWPEEFPKEKMSGEQQVPFINALTNDVEARLFLNVLNDGTIKNIPNDVVVEVPVKVNKNGIFPEKIQPDLPEKIKNY
YIIPRITRMEMALEAFITGNRNILEEVLVRDPRTKNYDDIPKLWDEIFDLPFNKEMKEHYRS

Specific function: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=460, Percent_Identity=25.2173913043478, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788029, Length=394, Percent_Identity=25.6345177664975, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 53872; Mature: 53741

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKISFIGAGSVRYTVKLVGDLAKTKELNGSLISFMDIDEERLNAVDNLAKRYTEEIGG
CCEEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
NLKFEKTTNREKSLKDADFVINTALYRAPGHEDGYVSYEIMRDVAEKYGYYRGIDSQEFN
CEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCH
MVSDYYTFTNYNHLKLSLDIAKSIEKICPNAWLIQTANPVFEITQLIKRLTNVKVVGFCH
HHHHHHEECCCCEEEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEEC
GVGGVHEVLKTLRLEENETDWQVAGVNHGIWLNRFLYKGEDGYQILDKWIEEESNNWEPK
CCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHCCCCCCCC
DPWDTHLAPAVIDMYKFYGLLPIGDTTRNSTWKHHHSLQAKKKWYGKFGAIDNEVERPKF
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
YEELRQTKRMIIQVSKDPNIKITENWPEEFPKEKMSGEQQVPFINALTNDVEARLFLNVL
HHHHHHHHHHEEEECCCCCEEECCCCCHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
NDGTIKNIPNDVVVEVPVKVNKNGIFPEKIQPDLPEKIKNYYIIPRITRMEMALEAFITG
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCEEECCCHHHHHHHHHHHHCC
NRNILEEVLVRDPRTKNYDDIPKLWDEIFDLPFNKEMKEHYRS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
PVKISFIGAGSVRYTVKLVGDLAKTKELNGSLISFMDIDEERLNAVDNLAKRYTEEIGG
CEEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
NLKFEKTTNREKSLKDADFVINTALYRAPGHEDGYVSYEIMRDVAEKYGYYRGIDSQEFN
CEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCH
MVSDYYTFTNYNHLKLSLDIAKSIEKICPNAWLIQTANPVFEITQLIKRLTNVKVVGFCH
HHHHHHEECCCCEEEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEEC
GVGGVHEVLKTLRLEENETDWQVAGVNHGIWLNRFLYKGEDGYQILDKWIEEESNNWEPK
CCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHCCCCCCCC
DPWDTHLAPAVIDMYKFYGLLPIGDTTRNSTWKHHHSLQAKKKWYGKFGAIDNEVERPKF
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
YEELRQTKRMIIQVSKDPNIKITENWPEEFPKEKMSGEQQVPFINALTNDVEARLFLNVL
HHHHHHHHHHEEEECCCCCEEECCCCCHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
NDGTIKNIPNDVVVEVPVKVNKNGIFPEKIQPDLPEKIKNYYIIPRITRMEMALEAFITG
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCEEECCCHHHHHHHHHHHHCC
NRNILEEVLVRDPRTKNYDDIPKLWDEIFDLPFNKEMKEHYRS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571; 10972187; 12062450; 12588867 [H]