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The map label for this gene is livF [H]

Identifier: 160901750

GI number: 160901750

Start: 280633

End: 281193

Strand: Direct

Name: livF [H]

Synonym: Pmob_0264

Alternate gene names: 160901750

Gene position: 280633-281193 (Clockwise)

Preceding gene: 160901748

Following gene: 160901751

Centisome position: 12.94

GC content: 39.75

Gene sequence:

>561_bases
TTGTGTTTGAAGCTAGCTATCATCTCTCCAATAAATTGGAGAGTGTTCTCGTTGGATTTTCACAAAATAGTAGAAAAGGG
GATAGCCCTAGTTCCTGAAGGTAGACGTATTTTCCCTAATCTCACTGTTTTAGAAAACCTCCGATTAGGTTCGTATTCTC
GCAAAGACTCTGAAAAAATAAACGAAGATTTCGATTGGGTACTTACACTTTTCCCGCGATTAAAGGAAAGACTTAAGCAA
CTTGGGGGTACACTCTCTGGAGGGGAACAACAAATGTTGGCGGTTGCAAGGGGGTTAATGTCAAGGCCAAAGGTTCTAAT
GCTCGACGAACCATCTCTGGGACTAGCTCCAATTCTAGTCAAAGAGGTTTTAGGAACTATAGAAAAAATAAGCCAACAGG
GAGTAACTATATTGCTTATAGAGCAAAATGCTGTTGGTGCGTTAAAAATCGCTAATTATGGTTACGTCTTAGAAACTGGA
AAAATTGTTCTTGAAGGTCCGGCAGAAGATCTCTTAGAAAACGATGAAGTTAGGAAAACTTATCTAGGAGTAACGGCTTA
A

Upstream 100 bases:

>100_bases
TTTAAGCATATTCAATCACCTTAATTCGATAATTGTATGATATAATACAATTGTATCACATATACATATAATTAACAACA
TTTTTTGATTCAAATTCGTT

Downstream 100 bases:

>100_bases
AACCTTGAAACTATTTCATACTTTTCAAGATTTCGAAGAAAGATATCAACAGTACAAGGATTTTCAAGATTTCCAAGATT
TCCAAAGATACCAAAACTAC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MCLKLAIISPINWRVFSLDFHKIVEKGIALVPEGRRIFPNLTVLENLRLGSYSRKDSEKINEDFDWVLTLFPRLKERLKQ
LGGTLSGGEQQMLAVARGLMSRPKVLMLDEPSLGLAPILVKEVLGTIEKISQQGVTILLIEQNAVGALKIANYGYVLETG
KIVLEGPAEDLLENDEVRKTYLGVTA

Sequences:

>Translated_186_residues
MCLKLAIISPINWRVFSLDFHKIVEKGIALVPEGRRIFPNLTVLENLRLGSYSRKDSEKINEDFDWVLTLFPRLKERLKQ
LGGTLSGGEQQMLAVARGLMSRPKVLMLDEPSLGLAPILVKEVLGTIEKISQQGVTILLIEQNAVGALKIANYGYVLETG
KIVLEGPAEDLLENDEVRKTYLGVTA
>Mature_186_residues
MCLKLAIISPINWRVFSLDFHKIVEKGIALVPEGRRIFPNLTVLENLRLGSYSRKDSEKINEDFDWVLTLFPRLKERLKQ
LGGTLSGGEQQMLAVARGLMSRPKVLMLDEPSLGLAPILVKEVLGTIEKISQQGVTILLIEQNAVGALKIANYGYVLETG
KIVLEGPAEDLLENDEVRKTYLGVTA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI148612844, Length=157, Percent_Identity=30.5732484076433, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI255708477, Length=157, Percent_Identity=30.5732484076433, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=162, Percent_Identity=53.7037037037037, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1789672, Length=158, Percent_Identity=31.6455696202532, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1789593, Length=167, Percent_Identity=29.940119760479, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787029, Length=143, Percent_Identity=31.4685314685315, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1786872, Length=151, Percent_Identity=29.1390728476821, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1788225, Length=164, Percent_Identity=31.7073170731707, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1789864, Length=181, Percent_Identity=28.7292817679558, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790162, Length=148, Percent_Identity=29.0540540540541, Blast_Score=73, Evalue=9e-15,
Organism=Escherichia coli, GI1790190, Length=167, Percent_Identity=31.1377245508982, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1787089, Length=170, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788644, Length=90, Percent_Identity=37.7777777777778, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1786253, Length=159, Percent_Identity=27.6729559748428, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787792, Length=162, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790467, Length=144, Percent_Identity=28.4722222222222, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87081791, Length=153, Percent_Identity=30.0653594771242, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 20671; Mature: 20671

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLKLAIISPINWRVFSLDFHKIVEKGIALVPEGRRIFPNLTVLENLRLGSYSRKDSEKI
CEEEEEEECCCCEEEEEEHHHHHHHCCCEECCCCCEECCCCHHHHCCCCCCCCCCCHHHH
NEDFDWVLTLFPRLKERLKQLGGTLSGGEQQMLAVARGLMSRPKVLMLDEPSLGLAPILV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHH
KEVLGTIEKISQQGVTILLIEQNAVGALKIANYGYVLETGKIVLEGPAEDLLENDEVRKT
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEECCEEEEECCHHHHHCCCCHHHH
YLGVTA
HCCCCC
>Mature Secondary Structure
MCLKLAIISPINWRVFSLDFHKIVEKGIALVPEGRRIFPNLTVLENLRLGSYSRKDSEKI
CEEEEEEECCCCEEEEEEHHHHHHHCCCEECCCCCEECCCCHHHHCCCCCCCCCCCHHHH
NEDFDWVLTLFPRLKERLKQLGGTLSGGEQQMLAVARGLMSRPKVLMLDEPSLGLAPILV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHH
KEVLGTIEKISQQGVTILLIEQNAVGALKIANYGYVLETGKIVLEGPAEDLLENDEVRKT
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEECCEEEEECCHHHHHCCCCHHHH
YLGVTA
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]