Click here to switch to the map view.

The map label for this gene is livJ [C]

Identifier: 160901751

GI number: 160901751

Start: 281796

End: 282926

Strand: Direct

Name: livJ [C]

Synonym: Pmob_0265

Alternate gene names: 160901751

Gene position: 281796-282926 (Clockwise)

Preceding gene: 160901750

Following gene: 160901752

Centisome position: 12.99

GC content: 41.2

Gene sequence:

>1131_bases
ATGAGAAAAACCGTTGTTTTGGTGTTTTTGGTAGTTATTATGACTTTCACACTCTTTGGTCAAGAAGTCATCAAAATTGG
TATGAACTTCGAACTAACAGGACCTGTTTCTGGGTATGGTCAAATGTCAAGAGACGGTGTAATGTTAGCCAACAAGTTGA
AACCAACAGTTAACATCGGGGGTAAAGAAATCAAAGTGGAAGTTGTAGCTGTCGATAACAAATCTGACAAAGCAGAATCA
GCTAACGCAATAAGAAGGTTGATAGATCACGAAAAGGTAGTTGCAGTTATTGGTCCTGCAACAAGTTCGGCAGCATTAGC
TGCAGCTTCAATAGCGGAAGAAAGGCAAATACCAATGGTGGTCAACACCGCCACTAACCCTTTAGTTGCCCAAAACAAAA
AGTATGTATTCAGGACCTGTTTTGAGGATACCTTGCAAGGTGCTCTACTTGCAACATTTGCCTGGGAAGAACTTGGAGCT
CAAAACGTCGCCATAATGGTAGATGTAGCACAAGATTACGTTGTGGGACTTGCTAATTATTTTGAAAAAGCTTTTGAACA
GTTTGGAGGAACGTATTTTACTGAATTTTATACTACTGGTGACCAAGATTTCACCGCTCAACTTACCGACGCCCTTTCAA
AAAATCCAGATGCTATTTTTATGCCAGGGTACTACGCTGAAATAGCCTTAATTTCCAAGCAAGCTAGAGATTTAGGTTTC
ACAGGACCCATGTTTGCTGGAGATGGTGCCGACGCACCAGAATTAATACAAATTGGTGGAGAATACGTAGAAGGCCTTTC
TTATACAACTTACTTTCATGAGGATGCAGAATTATCCCCTGCTACTAAACCTTTTGTAGAGGCCTATCAAGAGGAATACA
ACAGAAGAGCCGACGCATTTGGAGCTTTGGCCTATGATGCTTACATGGTAATAGTTAACGCTATTGAGTCTGCACAGTCC
ACAGATCCTGTAAAAATTAGAGACGCTTTAGCACAAACTAGAGACTTTCCGGGTGTCGCCGGTACAATTACGTATCCTCA
AGGCTCTGGAAACCCTATAAAACCAGCGGTAATAAACATGGTTGAAAACGGACAATTTGTTTTCCGTACCGTTATAAAAC
CCAGGATGTAA

Upstream 100 bases:

>100_bases
TATGAAATTCTGTTCACATAAGATAAAGACTATGAAAATTTTTTCTACACTTTATTAAGCAAAATAACATCCACACATAT
TATATGAGGAGGGGAAAAAC

Downstream 100 bases:

>100_bases
TATTTAACTATATTAAAGCCATCTCACAAAATGAGATGGCTATACTTTCTTGGATCCAGAAAGGGGGCTCATAATTGACT
ATCCAAATGTTCTTTCAACA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRKTVVLVFLVVIMTFTLFGQEVIKIGMNFELTGPVSGYGQMSRDGVMLANKLKPTVNIGGKEIKVEVVAVDNKSDKAES
ANAIRRLIDHEKVVAVIGPATSSAALAAASIAEERQIPMVVNTATNPLVAQNKKYVFRTCFEDTLQGALLATFAWEELGA
QNVAIMVDVAQDYVVGLANYFEKAFEQFGGTYFTEFYTTGDQDFTAQLTDALSKNPDAIFMPGYYAEIALISKQARDLGF
TGPMFAGDGADAPELIQIGGEYVEGLSYTTYFHEDAELSPATKPFVEAYQEEYNRRADAFGALAYDAYMVIVNAIESAQS
TDPVKIRDALAQTRDFPGVAGTITYPQGSGNPIKPAVINMVENGQFVFRTVIKPRM

Sequences:

>Translated_376_residues
MRKTVVLVFLVVIMTFTLFGQEVIKIGMNFELTGPVSGYGQMSRDGVMLANKLKPTVNIGGKEIKVEVVAVDNKSDKAES
ANAIRRLIDHEKVVAVIGPATSSAALAAASIAEERQIPMVVNTATNPLVAQNKKYVFRTCFEDTLQGALLATFAWEELGA
QNVAIMVDVAQDYVVGLANYFEKAFEQFGGTYFTEFYTTGDQDFTAQLTDALSKNPDAIFMPGYYAEIALISKQARDLGF
TGPMFAGDGADAPELIQIGGEYVEGLSYTTYFHEDAELSPATKPFVEAYQEEYNRRADAFGALAYDAYMVIVNAIESAQS
TDPVKIRDALAQTRDFPGVAGTITYPQGSGNPIKPAVINMVENGQFVFRTVIKPRM
>Mature_376_residues
MRKTVVLVFLVVIMTFTLFGQEVIKIGMNFELTGPVSGYGQMSRDGVMLANKLKPTVNIGGKEIKVEVVAVDNKSDKAES
ANAIRRLIDHEKVVAVIGPATSSAALAAASIAEERQIPMVVNTATNPLVAQNKKYVFRTCFEDTLQGALLATFAWEELGA
QNVAIMVDVAQDYVVGLANYFEKAFEQFGGTYFTEFYTTGDQDFTAQLTDALSKNPDAIFMPGYYAEIALISKQARDLGF
TGPMFAGDGADAPELIQIGGEYVEGLSYTTYFHEDAELSPATKPFVEAYQEEYNRRADAFGALAYDAYMVIVNAIESAQS
TDPVKIRDALAQTRDFPGVAGTITYPQGSGNPIKPAVINMVENGQFVFRTVIKPRM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=328, Percent_Identity=26.5243902439024, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994941, Length=332, Percent_Identity=27.710843373494, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40974; Mature: 40974

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVVLVFLVVIMTFTLFGQEVIKIGMNFELTGPVSGYGQMSRDGVMLANKLKPTVNIG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCEECC
GKEIKVEVVAVDNKSDKAESANAIRRLIDHEKVVAVIGPATSSAALAAASIAEERQIPMV
CEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEE
VNTATNPLVAQNKKYVFRTCFEDTLQGALLATFAWEELGAQNVAIMVDVAQDYVVGLANY
EECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHH
FEKAFEQFGGTYFTEFYTTGDQDFTAQLTDALSKNPDAIFMPGYYAEIALISKQARDLGF
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCC
TGPMFAGDGADAPELIQIGGEYVEGLSYTTYFHEDAELSPATKPFVEAYQEEYNRRADAF
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
GALAYDAYMVIVNAIESAQSTDPVKIRDALAQTRDFPGVAGTITYPQGSGNPIKPAVINM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHH
VENGQFVFRTVIKPRM
HCCCCEEEEECCCCCC
>Mature Secondary Structure
MRKTVVLVFLVVIMTFTLFGQEVIKIGMNFELTGPVSGYGQMSRDGVMLANKLKPTVNIG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCEECC
GKEIKVEVVAVDNKSDKAESANAIRRLIDHEKVVAVIGPATSSAALAAASIAEERQIPMV
CEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEE
VNTATNPLVAQNKKYVFRTCFEDTLQGALLATFAWEELGAQNVAIMVDVAQDYVVGLANY
EECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHH
FEKAFEQFGGTYFTEFYTTGDQDFTAQLTDALSKNPDAIFMPGYYAEIALISKQARDLGF
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCC
TGPMFAGDGADAPELIQIGGEYVEGLSYTTYFHEDAELSPATKPFVEAYQEEYNRRADAF
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
GALAYDAYMVIVNAIESAQSTDPVKIRDALAQTRDFPGVAGTITYPQGSGNPIKPAVINM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHH
VENGQFVFRTVIKPRM
HCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]