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The map label for this gene is mnmG [H]

Identifier: 160877619

GI number: 160877619

Start: 5340334

End: 5342223

Strand: Reverse

Name: mnmG [H]

Synonym: Sbal195_4518

Alternate gene names: 160877619

Gene position: 5342223-5340334 (Counterclockwise)

Preceding gene: 160877620

Following gene: 160877618

Centisome position: 99.91

GC content: 46.08

Gene sequence:

>1890_bases
ATGCATTTTCATGAACGGTTTGATGTAATTGTTGTTGGTGGTGGTCATGCCGGAACTGAAGCCGCGTTGGCAGCAGCAAG
AATGGGATCAAAAACATTATTACTAACGCACAATATCGATACGTTAGGACAAATGTCATGTAATCCAGCGATCGGCGGCA
TAGGCAAAGGCCACCTAGTGAAAGAAATTGACGCCTTAGGTGGCGCAATGGCGATCGCAACCGATTATGCAGGGATCCAA
TTTAGAACCTTGAATTCAAGCAAAGGCCCAGCAGTACGAGCGACACGTGCACAAGCTGACCGTGCGCTGTATCGTCAAAA
AATCCAAAACATATTACAAAACCAACCTAACTTACGTATTTTCCAACAAGCCGTTGATGACTTAATTGTTGAAAATCATC
AAGTCGTTGGCGTAGTGACTCAAATGGGATTAGCCTTTGAATCTCCAGCCGTTGTGTTAACAACAGGCACATTCTTAAGC
GGCAAAATTCACATAGGGTTAGAAAACTACAGCGGTGGCCGTGCAGGTGATCCACCAGCCATTGCCTTAGCCAATCGTTT
ACGTGAGTTACCGATCCGTGTGGGCCGTTTAAAAACTGGTACCCCGCCACGTATCGATGCAAACACGATCGATTTCTCTC
AAATGGCTGAACAAAAAGGTGATTCTCCGTTACCTGTGATGTCGTTTATGGGCGATGTCAGTCATCATCCAAAGCAGATT
TCTTGCTGGATCACTCATACCAATGAAAAAACCCATGAAATTATTCGTGGTGGTTTAGACAGAAGCCCAATGTATTCAGG
CGTAATCGAGGGTATTGGCCCGCGTTATTGCCCATCGATTGAAGACAAGATCCATCGCTTTGCAGATAAATCTTCGCATC
AGATCTTTATCGAACCTGAAGGGTTAAATACCAGTGAGATCTACCCTAACGGGATCTCGACCAGCTTACCTTTCGATGTG
CAGCTGAATTTAGTACGTTCGATCAAGGGCATGGAAAACGCAGAAATCATGCGTCCAGGTTATGCGATCGAATATGATTA
TTTCGATCCAAGGGATCTGAAAAACTCATTAGAAACCAAAGCGATCAACGGGTTATTCTTCGCTGGCCAAATAAACGGCA
CAACCGGTTATGAAGAAGCCGGTGCTCAAGGTTTATTAGCGGGCATGAATGCGTCGCTGCAAGTGCAAGGCAAAGAAGCT
TGGTGTCCACGTCGTGACGAAGCGTATCTTGGGGTGCTGGTTGACGATCTGTCGACTCTGGGTACCAAAGAACCGTACCG
CATGTTTACTAGCCGTGCCGAATACCGTTTGTTACTCCGTGAAGATAACGCCGACATTCGCTTAACGGCAAAAGGTCGCG
AACTCGGTTTAGTCGACGACGCTCGCTGGGCAGCATTTAGTGAGAAGCTAGAATCCATTGAACTTGAACTGCAAAGGCTA
CGTGGCCAATGGGTGCATCCAAATTCGCCTTTGATCCATGCGTTAAACCCACATTTGAATACGCCGATCTCGCGCGAAGC
GTCATTTGAAGAATTACTGCGTCGTCCAGAAATGGATTACAGCAAGTTAATGCAGATCGAAGGTTTTGGTCCTGGGCTTG
AAGATCCACAAGCAGCTGAACAAGTTCAGATCCAAGTGAAGTACTCTGGTTATATTCAACGCCAGCAGGAAGAGATCAAC
AAAGCCGTGCGTAATGAGAACACAGGTTTACCTTTGACGCTCGATTACAAAGAAGTACCGGGCTTGTCGAACGAAGTGAT
TGCTAAGTTAAATAACCACAAACCAGAAACCATAGGCCAAGCGTCACGGATCTCTGGTATTACCCCTGCGGCTATTTCAA
TTTTGTTGGTTCACCTCAAAAAGCGTGGCTTATTACGTAAGAGTGCGTAA

Upstream 100 bases:

>100_bases
CATCTCGATCGAACAGCGTAAAATGATCATTCACCTAGCGCAAAGCAAATGCTAAAATACTCGGCTCGTACTGAGTCTCT
TAGCAATTGAAGGTAGTGGC

Downstream 100 bases:

>100_bases
AAGCTAGCTTAATCGCTGCTTAGGTCATAGAAGGGAAGCATTCAGGCTTCCCTTCATCGTTTTCGGGCTTCATAATAGCC
TTCGCGTTCATAATCGTATC

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQPNLRIFQQAVDDLIVENHQVVGVVTQMGLAFESPAVVLTTGTFLS
GKIHIGLENYSGGRAGDPPAIALANRLRELPIRVGRLKTGTPPRIDANTIDFSQMAEQKGDSPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHEIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKSSHQIFIEPEGLNTSEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAINGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADIRLTAKGRELGLVDDARWAAFSEKLESIELELQRL
RGQWVHPNSPLIHALNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLEDPQAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLTLDYKEVPGLSNEVIAKLNNHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA

Sequences:

>Translated_629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQPNLRIFQQAVDDLIVENHQVVGVVTQMGLAFESPAVVLTTGTFLS
GKIHIGLENYSGGRAGDPPAIALANRLRELPIRVGRLKTGTPPRIDANTIDFSQMAEQKGDSPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHEIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKSSHQIFIEPEGLNTSEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAINGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADIRLTAKGRELGLVDDARWAAFSEKLESIELELQRL
RGQWVHPNSPLIHALNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLEDPQAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLTLDYKEVPGLSNEVIAKLNNHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA
>Mature_629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQPNLRIFQQAVDDLIVENHQVVGVVTQMGLAFESPAVVLTTGTFLS
GKIHIGLENYSGGRAGDPPAIALANRLRELPIRVGRLKTGTPPRIDANTIDFSQMAEQKGDSPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHEIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKSSHQIFIEPEGLNTSEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAINGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADIRLTAKGRELGLVDDARWAAFSEKLESIELELQRL
RGQWVHPNSPLIHALNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLEDPQAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLTLDYKEVPGLSNEVIAKLNNHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=46.6772151898734, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=44.6455505279035, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=43.8988095238095, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=75.0397456279809, Blast_Score=986, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=638, Percent_Identity=41.2225705329154, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321202, Length=632, Percent_Identity=47.626582278481, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24658174, Length=643, Percent_Identity=46.656298600311, Blast_Score=516, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69287; Mature: 69287

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAIATDYAGIQFRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQPNLRI
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
FQQAVDDLIVENHQVVGVVTQMGLAFESPAVVLTTGTFLSGKIHIGLENYSGGRAGDPPA
HHHHHHHHHHCCCEEEEEHHHHCCEECCCEEEEEECCEEEEEEEEEEECCCCCCCCCCHH
IALANRLRELPIRVGRLKTGTPPRIDANTIDFSQMAEQKGDSPLPVMSFMGDVSHHPKQI
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
SCWITHTNEKTHEIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKSSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTSEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
AINGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWCPRRDEAYLGVLVDDLSTL
HHCCEEEEEEECCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADIRLTAKGRELGLVDDARWAAFSEKLESIELELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHH
RGQWVHPNSPLIHALNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLEDPQAAE
CCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
QVQIQVKYSGYIQRQQEEINKAVRNENTGLPLTLDYKEVPGLSNEVIAKLNNHKPETIGQ
EEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECHHHCCCCCHHHHHHHCCCCCHHHCC
ASRISGITPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAIATDYAGIQFRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQPNLRI
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
FQQAVDDLIVENHQVVGVVTQMGLAFESPAVVLTTGTFLSGKIHIGLENYSGGRAGDPPA
HHHHHHHHHHCCCEEEEEHHHHCCEECCCEEEEEECCEEEEEEEEEEECCCCCCCCCCHH
IALANRLRELPIRVGRLKTGTPPRIDANTIDFSQMAEQKGDSPLPVMSFMGDVSHHPKQI
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
SCWITHTNEKTHEIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKSSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTSEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
AINGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWCPRRDEAYLGVLVDDLSTL
HHCCEEEEEEECCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADIRLTAKGRELGLVDDARWAAFSEKLESIELELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHH
RGQWVHPNSPLIHALNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLEDPQAAE
CCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
QVQIQVKYSGYIQRQQEEINKAVRNENTGLPLTLDYKEVPGLSNEVIAKLNNHKPETIGQ
EEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECHHHCCCCCHHHHHHHCCCCCHHHCC
ASRISGITPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA