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The map label for this gene is gidB

Identifier: 160877618

GI number: 160877618

Start: 5339596

End: 5340216

Strand: Reverse

Name: gidB

Synonym: Sbal195_4517

Alternate gene names: 160877618

Gene position: 5340216-5339596 (Counterclockwise)

Preceding gene: 160877619

Following gene: 160877617

Centisome position: 99.87

GC content: 41.55

Gene sequence:

>621_bases
GTGTTATCTGCCCAGTTAGAGGCTTATTTAGCCGAAATTAATTTGCCCGCAACCGCCGAGCAAAAAAAACAACTAATTGA
TTTTGTTGGCATGCTCAACAAATGGAATAAAGCCTATAACCTGACATCGGTGAGAGATCCCGAAGCCATGTTGATCCGTC
ATATCATGGACAGCTTAGTGGTTTCAAAACATCTGCAAGGTGAACGTTTTATCGATGTGGGCACTGGACCCGGTTTACCT
GGGATCCCGTTAGCGATTATGAATCCCGATAAACAATTTGTGTTATTGGACAGCTTAGGTAAACGCATTCGTTTTCAAAA
ACAAGTGTCCTTTGAATTAGGCATTCATAACATCAGCTCAGTTGAAAGCCGCGTAGAAGCTTATCAACCTGAGCAAAAAT
TCGACGGCGTATTAAGTCGTGCATTTGCTTCAATCCAAGATATGCTGACTTGGTGCCATCATTTACCCGCCGAGCATGGG
CAGTTTTATGCCCTTAAAGGCCAGTTAAATGATGAAGAGATGCAACATATTCCAAGTGGATTTGCAGTGAAAGAAGTCAT
CGAGCTCAAGGTGCCTAAACTCGATGAACAAAGACATTTATTGAAAATAATCAAAGAATAA

Upstream 100 bases:

>100_bases
GCTTAGGTCATAGAAGGGAAGCATTCAGGCTTCCCTTCATCGTTTTCGGGCTTCATAATAGCCTTCGCGTTCATAATCGT
ATCTTTGTTGAGGTTGCTCA

Downstream 100 bases:

>100_bases
CAGGCTTGAAAGTGATGAATGAATCATTTTTAAGTAAGGGCAATAAACCAGCATTTACATACAGGGTGATATTGTGGGTA
AAGTGATTGCCGTGGCTAAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLSAQLEAYLAEINLPATAEQKKQLIDFVGMLNKWNKAYNLTSVRDPEAMLIRHIMDSLVVSKHLQGERFIDVGTGPGLP
GIPLAIMNPDKQFVLLDSLGKRIRFQKQVSFELGIHNISSVESRVEAYQPEQKFDGVLSRAFASIQDMLTWCHHLPAEHG
QFYALKGQLNDEEMQHIPSGFAVKEVIELKVPKLDEQRHLLKIIKE

Sequences:

>Translated_206_residues
MLSAQLEAYLAEINLPATAEQKKQLIDFVGMLNKWNKAYNLTSVRDPEAMLIRHIMDSLVVSKHLQGERFIDVGTGPGLP
GIPLAIMNPDKQFVLLDSLGKRIRFQKQVSFELGIHNISSVESRVEAYQPEQKFDGVLSRAFASIQDMLTWCHHLPAEHG
QFYALKGQLNDEEMQHIPSGFAVKEVIELKVPKLDEQRHLLKIIKE
>Mature_206_residues
MLSAQLEAYLAEINLPATAEQKKQLIDFVGMLNKWNKAYNLTSVRDPEAMLIRHIMDSLVVSKHLQGERFIDVGTGPGLP
GIPLAIMNPDKQFVLLDSLGKRIRFQKQVSFELGIHNISSVESRVEAYQPEQKFDGVLSRAFASIQDMLTWCHHLPAEHG
QFYALKGQLNDEEMQHIPSGFAVKEVIELKVPKLDEQRHLLKIIKE

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=199, Percent_Identity=59.7989949748744, Blast_Score=258, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SHEB2 (B8EDW0)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002360209.1
- ProteinModelPortal:   B8EDW0
- SMR:   B8EDW0
- GeneID:   7089125
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_4320
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23383; Mature: 23383

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQLEAYLAEINLPATAEQKKQLIDFVGMLNKWNKAYNLTSVRDPEAMLIRHIMDSLV
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSKHLQGERFIDVGTGPGLPGIPLAIMNPDKQFVLLDSLGKRIRFQKQVSFELGIHNISS
HHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
VESRVEAYQPEQKFDGVLSRAFASIQDMLTWCHHLPAEHGQFYALKGQLNDEEMQHIPSG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCC
FAVKEVIELKVPKLDEQRHLLKIIKE
HHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLSAQLEAYLAEINLPATAEQKKQLIDFVGMLNKWNKAYNLTSVRDPEAMLIRHIMDSLV
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSKHLQGERFIDVGTGPGLPGIPLAIMNPDKQFVLLDSLGKRIRFQKQVSFELGIHNISS
HHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
VESRVEAYQPEQKFDGVLSRAFASIQDMLTWCHHLPAEHGQFYALKGQLNDEEMQHIPSG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCC
FAVKEVIELKVPKLDEQRHLLKIIKE
HHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA